+Open data
-Basic information
Entry | Database: PDB / ID: 1ju0 | ||||||||||||||||||
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Title | NMR solution structure of a DNA kissing complex | ||||||||||||||||||
Components | 5'-D(*Keywords | DNA / HIV / SL1 / kissing complex / loop-loop dimer | Function / homology | DNA / DNA (> 10) | Function and homology information Method | SOLUTION NMR / simulated annealing matrix relaxation chemical shift back calculation | Authors | Barbault, F. / Huynh-Dinh, T. / Paoletti, J. / Lancelot, G. | Citation | Journal: J.Biomol.Struct.Dyn. / Year: 2002 | Title: A new peculiar DNA structure: NMR solution structure of a DNA kissing complex. Authors: Barbault, F. / Huynh-Dinh, T. / Paoletti, J. / Lanceloti, G. History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1ju0.cif.gz | 423.5 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1ju0.ent.gz | 347.6 KB | Display | PDB format |
PDBx/mmJSON format | 1ju0.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/ju/1ju0 ftp://data.pdbj.org/pub/pdb/validation_reports/ju/1ju0 | HTTPS FTP |
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-Related structure data
-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: DNA chain | Mass: 7091.574 Da / Num. of mol.: 2 / Source method: obtained synthetically / Details: DNA reverse transcripted sequence |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||||||
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NMR experiment |
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-Sample preparation
Details |
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Sample conditions |
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-NMR measurement
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M |
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Radiation wavelength | Relative weight: 1 |
NMR spectrometer | Type: Varian INOVA / Manufacturer: Varian / Model: INOVA / Field strength: 600 MHz |
-Processing
NMR software |
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Refinement | Method: simulated annealing matrix relaxation chemical shift back calculation Software ordinal: 1 | ||||||||||||||||||||||||||||||||
NMR representative | Selection criteria: lowest energy | ||||||||||||||||||||||||||||||||
NMR ensemble | Conformer selection criteria: back calculated data agree with experimental NOESY spectrum, structures with acceptable covalent geometry, structures with the least restraint violations, structures with the lowest energy Conformers calculated total number: 16 / Conformers submitted total number: 16 |