[English] 日本語
![](img/lk-miru.gif)
- PDB-1jny: Crystal structure of Sulfolobus solfataricus elongation factor 1 ... -
+
Open data
-
Basic information
Entry | Database: PDB / ID: 1jny | ||||||
---|---|---|---|---|---|---|---|
Title | Crystal structure of Sulfolobus solfataricus elongation factor 1 alpha in complex with GDP | ||||||
![]() | Elongation factor 1-alpha | ||||||
![]() | TRANSLATION / GTPASE / ALPHA/BETA STRUCTURE / PROTEIN BIOSYNTHESIS | ||||||
Function / homology | ![]() sulfur compound metabolic process / translation elongation factor activity / GTPase activity / GTP binding / cytoplasm Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Vitagliano, L. / Masullo, M. / Sica, F. / Zagari, A. / Bocchini, V. | ||||||
![]() | ![]() Title: The crystal structure of Sulfolobus solfataricus elongation factor 1alpha in complex with GDP reveals novel features in nucleotide binding and exchange. Authors: Vitagliano, L. / Masullo, M. / Sica, F. / Zagari, A. / Bocchini, V. #1: ![]() Title: Crystallization of a hyperthermophilic archeal elongation factor 1 alpha Authors: Zagari, A. / Sica, F. / Scarano, G. / Vitagliano, L. / Bocchini, V. | ||||||
History |
|
-
Structure visualization
Structure viewer | Molecule: ![]() ![]() |
---|
-
Downloads & links
-
Download
PDBx/mmCIF format | ![]() | 182.2 KB | Display | ![]() |
---|---|---|---|---|
PDB format | ![]() | 144 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 1.1 MB | Display | ![]() |
---|---|---|---|---|
Full document | ![]() | 1.1 MB | Display | |
Data in XML | ![]() | 38.6 KB | Display | |
Data in CIF | ![]() | 55.1 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Similar structure data |
---|
-
Links
-
Assembly
Deposited unit | ![]()
| ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
1 | ![]()
| ||||||||||
2 | ![]()
| ||||||||||
Unit cell |
|
-
Components
#1: Protein | Mass: 48539.059 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) ![]() ![]() #2: Chemical | #3: Water | ChemComp-HOH / | |
---|
-Experimental details
-Experiment
Experiment | Method: ![]() |
---|
-
Sample preparation
Crystal | Density Matthews: 2.92 Å3/Da / Density % sol: 57.88 % |
---|---|
Crystal grow | Temperature: 277 K / Method: microbatch under oil / pH: 5.6 Details: PEG 4000, magnesium chloride, propan-2-ol, citrate buffer, pH 5.6, MICROBATCH UNDER OIL at 277K |
Crystal grow | *PLUS Details: Zagari, A., (1994) J. Mol. Biol., 242, 175. |
-Data collection
Diffraction | Mean temperature: 298 K |
---|---|
Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: MARRESEARCH / Detector: IMAGE PLATE / Date: Sep 6, 1998 |
Radiation | Monochromator: Si / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1 Å / Relative weight: 1 |
Reflection | Resolution: 1.8→20 Å / Num. all: 101657 / Num. obs: 101657 / % possible obs: 98.6 % / Observed criterion σ(F): 0 / Observed criterion σ(I): 0 / Redundancy: 7.5 % / Rmerge(I) obs: 0.034 / Net I/σ(I): 38.7 |
Reflection shell | Resolution: 1.8→1.85 Å / Rmerge(I) obs: 0.172 / Mean I/σ(I) obs: 8.7 / Num. unique all: 9857 / % possible all: 95.5 |
Reflection | *PLUS Num. measured all: 762914 |
Reflection shell | *PLUS Lowest resolution: 1.86 Å / % possible obs: 95.5 % |
-
Processing
Software |
| |||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Refinement | Method to determine structure: ![]()
| |||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.8→20 Å
| |||||||||||||||||||||||||
Refine LS restraints |
| |||||||||||||||||||||||||
Software | *PLUS Name: ![]() | |||||||||||||||||||||||||
Refinement | *PLUS Highest resolution: 1.8 Å / Lowest resolution: 20 Å / σ(F): 0 / % reflection Rfree: 8 % / Rfactor Rwork: 0.22 | |||||||||||||||||||||||||
Solvent computation | *PLUS | |||||||||||||||||||||||||
Displacement parameters | *PLUS | |||||||||||||||||||||||||
Refine LS restraints | *PLUS
|