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Open data
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Basic information
Entry | Database: PDB / ID: 1jbv | ||||||
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Title | FPGS-AMPPCP complex | ||||||
![]() | FOLYLPOLYGLUTAMATE SYNTHASE | ||||||
![]() | LIGASE / FPGS AMPPCP complex | ||||||
Function / homology | ![]() tetrahydrofolate synthase / dihydrofolate synthase activity / tetrahydrofolylpolyglutamate synthase activity / one-carbon metabolic process / ATP binding / metal ion binding / cytosol Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() | ||||||
![]() | Sun, X. / Cross, J.A. / Bognar, A.L. / Baker, E.N. / Smith, C.A. | ||||||
![]() | ![]() Title: Folate-binding triggers the activation of folylpolyglutamate synthetase. Authors: Sun, X. / Cross, J.A. / Bognar, A.L. / Baker, E.N. / Smith, C.A. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 100.4 KB | Display | ![]() |
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PDB format | ![]() | 73.5 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 452.4 KB | Display | ![]() |
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Full document | ![]() | 462.5 KB | Display | |
Data in XML | ![]() | 11 KB | Display | |
Data in CIF | ![]() | 17.6 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 1jbwC ![]() 1fgsS S: Starting model for refinement C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 |
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Unit cell |
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Components
#1: Protein | Mass: 46697.016 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() | ||||
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#2: Chemical | #3: Chemical | ChemComp-ACP / | #4: Water | ChemComp-HOH / | |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.06 Å3/Da / Density % sol: 40.31 % | ||||||||||||||||||||||||||||||
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Crystal grow | *PLUS pH: 5.3 / Method: vapor diffusion, hanging dropDetails: Sun, X., (1998) Proc. Natl. Acad. Sci. USA, 95, 6647. | ||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 113 K |
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Diffraction source | Source: ![]() |
Detector | Type: MARRESEARCH / Detector: IMAGE PLATE / Date: Jan 10, 2000 / Details: mirrors |
Radiation | Monochromator: Mar mirrors / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.5418 Å / Relative weight: 1 |
Reflection | Resolution: 1.9→100 Å / Num. all: 28314 / Num. obs: 28314 / % possible obs: 94 % / Redundancy: 6.9 % / Rmerge(I) obs: 0.051 / Net I/σ(I): 23.4 |
Reflection shell | Resolution: 1.9→1.97 Å / Rmerge(I) obs: 0.295 / Mean I/σ(I) obs: 3.2 / % possible all: 86.1 |
Reflection | *PLUS Highest resolution: 1.9 Å / Lowest resolution: 100 Å / % possible obs: 94 % / Num. measured all: 195787 |
Reflection shell | *PLUS Highest resolution: 1.9 Å / % possible obs: 86.1 % |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: PDB ENTRY 1FGS Resolution: 1.95→100 Å / Cross valid method: THROUGHOUT / Stereochemistry target values: Engh & Huber
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Refinement step | Cycle: LAST / Resolution: 1.95→100 Å
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Refine LS restraints |
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Software | *PLUS Name: CNS / Version: 1 / Classification: refinement | |||||||||||||||||||||||||
Refinement | *PLUS Lowest resolution: 100 Å / % reflection Rfree: 5 % / Rfactor obs: 0.194 | |||||||||||||||||||||||||
Solvent computation | *PLUS | |||||||||||||||||||||||||
Displacement parameters | *PLUS |