+Open data
-Basic information
Entry | Database: PDB / ID: 1j9o | ||||||
---|---|---|---|---|---|---|---|
Title | SOLUTION STRUCTURE OF HUMAN LYMPHOTACTIN | ||||||
Components | LYMPHOTACTINXCL1 | ||||||
Keywords | CYTOKINE / CHEMOKINE | ||||||
Function / homology | Function and homology information mature natural killer cell chemotaxis / positive regulation of granzyme A production / negative regulation of T-helper 1 cell activation / positive regulation of immunoglobulin production in mucosal tissue / positive regulation of thymocyte migration / positive regulation of granzyme B production / negative regulation of T-helper 1 type immune response / positive regulation of B cell chemotaxis / negative regulation of T cell cytokine production / positive regulation of natural killer cell chemotaxis ...mature natural killer cell chemotaxis / positive regulation of granzyme A production / negative regulation of T-helper 1 cell activation / positive regulation of immunoglobulin production in mucosal tissue / positive regulation of thymocyte migration / positive regulation of granzyme B production / negative regulation of T-helper 1 type immune response / positive regulation of B cell chemotaxis / negative regulation of T cell cytokine production / positive regulation of natural killer cell chemotaxis / chemokine receptor binding / positive regulation of T-helper 1 cell cytokine production / positive regulation of transforming growth factor beta production / positive regulation of T cell chemotaxis / positive regulation of T-helper 2 cell cytokine production / CCR chemokine receptor binding / lymphocyte chemotaxis / positive regulation of CD4-positive, alpha-beta T cell proliferation / negative regulation of CD4-positive, alpha-beta T cell proliferation / eosinophil chemotaxis / chemokine-mediated signaling pathway / positive regulation of neutrophil chemotaxis / Chemokine receptors bind chemokines / chemokine activity / positive regulation of leukocyte chemotaxis / negative regulation of interleukin-2 production / positive regulation of CD8-positive, alpha-beta T cell proliferation / monocyte chemotaxis / positive regulation of interleukin-10 production / negative regulation of type II interferon production / cellular response to interleukin-1 / cellular response to interleukin-4 / release of sequestered calcium ion into cytosol / cellular response to transforming growth factor beta stimulus / neutrophil chemotaxis / positive regulation of release of sequestered calcium ion into cytosol / response to virus / negative regulation of DNA-binding transcription factor activity / positive regulation of T cell cytokine production / cellular response to type II interferon / positive regulation of T cell mediated cytotoxicity / cell-cell signaling / cellular response to tumor necrosis factor / regulation of inflammatory response / G alpha (q) signalling events / positive regulation of ERK1 and ERK2 cascade / inflammatory response / G protein-coupled receptor signaling pathway / negative regulation of DNA-templated transcription / signal transduction / protein homodimerization activity / extracellular space / extracellular region Similarity search - Function | ||||||
Biological species | Homo sapiens (human) | ||||||
Method | SOLUTION NMR / torsion angle dynamics | ||||||
Authors | Kuloglu, E.S. / McCaslin, D.R. / Kitabwalla, M. / Pauza, C.D. / Markley, J.L. / Volkman, B.F. | ||||||
Citation | Journal: Biochemistry / Year: 2001 Title: Monomeric solution structure of the prototypical 'C' chemokine lymphotactin. Authors: Kuloglu, E.S. / McCaslin, D.R. / Kitabwalla, M. / Pauza, C.D. / Markley, J.L. / Volkman, B.F. | ||||||
History |
|
-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
---|
-Downloads & links
-Download
PDBx/mmCIF format | 1j9o.cif.gz | 35.9 KB | Display | PDBx/mmCIF format |
---|---|---|---|---|
PDB format | pdb1j9o.ent.gz | 27.7 KB | Display | PDB format |
PDBx/mmJSON format | 1j9o.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/j9/1j9o ftp://data.pdbj.org/pub/pdb/validation_reports/j9/1j9o | HTTPS FTP |
---|
-Related structure data
-Links
-Assembly
Deposited unit |
| |||||||||
---|---|---|---|---|---|---|---|---|---|---|
1 |
| |||||||||
NMR ensembles |
|
-Components
#1: Protein | Mass: 10286.789 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Plasmid: PET3A / Production host: Escherichia coli (E. coli) / Strain (production host): BL21(DE3)PLYSS / References: UniProt: P47992 |
---|
-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
NMR experiment |
|
-Sample preparation
Crystal | Description: THE STRUCTURE WAS DETERMINED USING TRIPLE- RESONANCE NMR SPECTROSCOPY. | ||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Details |
| ||||||||||||
Sample conditions | Ionic strength: 200mM NaCl / pH: 6.0 / Temperature: 283 K | ||||||||||||
Crystal grow | *PLUS Method: other / Details: NMR |
-NMR measurement
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M | |||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Radiation wavelength | Relative weight: 1 | |||||||||||||||
NMR spectrometer |
|
-Processing
Software | Name: DYANA / Classification: refinement | ||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
NMR software |
| ||||||||||||||||||||||||
Refinement | Method: torsion angle dynamics / Software ordinal: 1 Details: THE STRUCTURES ARE BASED ON A TOTAL OF 1258 NOE-DERIVED DISTANCE CONSTRAINTS, 60 DIHEDRAL ANGLE CONSTRAINTS AND 4 DISULFIDE BOND CONSTRAINTS. | ||||||||||||||||||||||||
NMR ensemble | Conformer selection criteria: STRUCTURES WITH THE LOWEST ENERGY, TARGET FUNCTION Conformers calculated total number: 50 / Conformers submitted total number: 1 |