+データを開く
-基本情報
登録情報 | データベース: PDB / ID: 1j8i | ||||||
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タイトル | Solution Structure of Human Lymphotactin | ||||||
要素 | LymphotactinXCL1 | ||||||
キーワード | CYTOKINE (サイトカイン) / Chemokine (ケモカイン) | ||||||
機能・相同性 | 機能・相同性情報 mature natural killer cell chemotaxis / positive regulation of granzyme A production / negative regulation of T-helper 1 cell activation / positive regulation of immunoglobulin production in mucosal tissue / positive regulation of thymocyte migration / positive regulation of granzyme B production / negative regulation of T-helper 1 type immune response / positive regulation of B cell chemotaxis / negative regulation of T cell cytokine production / positive regulation of natural killer cell chemotaxis ...mature natural killer cell chemotaxis / positive regulation of granzyme A production / negative regulation of T-helper 1 cell activation / positive regulation of immunoglobulin production in mucosal tissue / positive regulation of thymocyte migration / positive regulation of granzyme B production / negative regulation of T-helper 1 type immune response / positive regulation of B cell chemotaxis / negative regulation of T cell cytokine production / positive regulation of natural killer cell chemotaxis / chemokine receptor binding / positive regulation of T-helper 1 cell cytokine production / positive regulation of transforming growth factor beta production / positive regulation of T cell chemotaxis / positive regulation of T-helper 2 cell cytokine production / CCR chemokine receptor binding / lymphocyte chemotaxis / positive regulation of CD4-positive, alpha-beta T cell proliferation / negative regulation of CD4-positive, alpha-beta T cell proliferation / eosinophil chemotaxis / chemokine-mediated signaling pathway / positive regulation of neutrophil chemotaxis / Chemokine receptors bind chemokines / chemokine activity / positive regulation of leukocyte chemotaxis / negative regulation of interleukin-2 production / positive regulation of CD8-positive, alpha-beta T cell proliferation / monocyte chemotaxis / positive regulation of interleukin-10 production / negative regulation of type II interferon production / cellular response to interleukin-1 / cellular response to interleukin-4 / release of sequestered calcium ion into cytosol / cellular response to transforming growth factor beta stimulus / 好中球 / positive regulation of release of sequestered calcium ion into cytosol / response to virus / negative regulation of DNA-binding transcription factor activity / positive regulation of T cell cytokine production / cellular response to type II interferon / positive regulation of T cell mediated cytotoxicity / cell-cell signaling / cellular response to tumor necrosis factor / regulation of inflammatory response / G alpha (q) signalling events / positive regulation of ERK1 and ERK2 cascade / 炎症 / G protein-coupled receptor signaling pathway / negative regulation of DNA-templated transcription / シグナル伝達 / protein homodimerization activity / extracellular space / extracellular region 類似検索 - 分子機能 | ||||||
生物種 | Homo sapiens (ヒト) | ||||||
手法 | 溶液NMR / torsion angle dynamics | ||||||
データ登録者 | Kuloglu, E.S. / McCaslin, D.R. / Markley, J.L. / Pauza, C.D. / Volkman, B.F. | ||||||
引用 | ジャーナル: Biochemistry / 年: 2001 タイトル: Monomeric solution structure of the prototypical 'C' chemokine lymphotactin. 著者: Kuloglu, E.S. / McCaslin, D.R. / Kitabwalla, M. / Pauza, C.D. / Markley, J.L. / Volkman, B.F. | ||||||
履歴 |
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-構造の表示
構造ビューア | 分子: MolmilJmol/JSmol |
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-ダウンロードとリンク
-ダウンロード
PDBx/mmCIF形式 | 1j8i.cif.gz | 569.6 KB | 表示 | PDBx/mmCIF形式 |
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PDB形式 | pdb1j8i.ent.gz | 493 KB | 表示 | PDB形式 |
PDBx/mmJSON形式 | 1j8i.json.gz | ツリー表示 | PDBx/mmJSON形式 | |
その他 | その他のダウンロード |
-検証レポート
アーカイブディレクトリ | https://data.pdbj.org/pub/pdb/validation_reports/j8/1j8i ftp://data.pdbj.org/pub/pdb/validation_reports/j8/1j8i | HTTPS FTP |
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-関連構造データ
-リンク
-集合体
登録構造単位 |
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1 |
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NMR アンサンブル |
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-要素
#1: タンパク質 | 分子量: 10286.789 Da / 分子数: 1 / 由来タイプ: 組換発現 / 由来: (組換発現) Homo sapiens (ヒト) / プラスミド: pET3A / 発現宿主: Escherichia coli (大腸菌) / 株 (発現宿主): BL21(DE3)pLysS / 参照: UniProt: P47992 |
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-実験情報
-実験
実験 | 手法: 溶液NMR | ||||||||||||||||
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NMR実験 |
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NMR実験の詳細 | Text: The structure was determined using triple-resonance NMR spectroscopy. |
-試料調製
詳細 |
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試料状態 | イオン強度: 200 mM sodium chloride / pH: 6.0 / 温度: 283 K |
-NMR測定
放射 | プロトコル: SINGLE WAVELENGTH / 単色(M)・ラウエ(L): M | |||||||||||||||
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放射波長 | 相対比: 1 | |||||||||||||||
NMRスペクトロメーター |
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-解析
NMR software |
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精密化 | 手法: torsion angle dynamics / ソフトェア番号: 1 詳細: The structures are based on a total of 1258 NOE-derived distance constraints, 60 dihedral angle constraints and 4 disulfide bond constraints. | ||||||||||||||||||||||||||||
NMRアンサンブル | コンフォーマー選択の基準: structures with the lowest energy, target function 計算したコンフォーマーの数: 50 / 登録したコンフォーマーの数: 20 |