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データを開く
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基本情報
登録情報 | データベース: PDB / ID: 1j73 | ||||||
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タイトル | Crystal structure of an unstable insulin analog with native activity. | ||||||
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![]() | HORMONE/GROWTH FACTOR / A8-Diaminobutyric Acid human insulin / hormone-receptor interface / HORMONE-GROWTH FACTOR COMPLEX | ||||||
機能・相同性 | ![]() negative regulation of NAD(P)H oxidase activity / negative regulation of glycogen catabolic process / positive regulation of nitric oxide mediated signal transduction / negative regulation of feeding behavior / negative regulation of fatty acid metabolic process / Signaling by Insulin receptor / IRS activation / Insulin processing / regulation of protein secretion / positive regulation of peptide hormone secretion ...negative regulation of NAD(P)H oxidase activity / negative regulation of glycogen catabolic process / positive regulation of nitric oxide mediated signal transduction / negative regulation of feeding behavior / negative regulation of fatty acid metabolic process / Signaling by Insulin receptor / IRS activation / Insulin processing / regulation of protein secretion / positive regulation of peptide hormone secretion / positive regulation of protein autophosphorylation / positive regulation of respiratory burst / Regulation of gene expression in beta cells / negative regulation of acute inflammatory response / regulation of transmembrane transporter activity / alpha-beta T cell activation / positive regulation of dendritic spine maintenance / Synthesis, secretion, and deacylation of Ghrelin / negative regulation of respiratory burst involved in inflammatory response / negative regulation of protein secretion / activation of protein kinase B activity / positive regulation of insulin receptor signaling pathway / negative regulation of gluconeogenesis / positive regulation of glycogen biosynthetic process / fatty acid homeostasis / Signal attenuation / FOXO-mediated transcription of oxidative stress, metabolic and neuronal genes / negative regulation of lipid catabolic process / positive regulation of lipid biosynthetic process / regulation of protein localization to plasma membrane / negative regulation of oxidative stress-induced intrinsic apoptotic signaling pathway / transport vesicle / nitric oxide-cGMP-mediated signaling / COPI-mediated anterograde transport / positive regulation of nitric-oxide synthase activity / Insulin receptor recycling / negative regulation of reactive oxygen species biosynthetic process / insulin-like growth factor receptor binding / positive regulation of brown fat cell differentiation / NPAS4 regulates expression of target genes / endoplasmic reticulum-Golgi intermediate compartment membrane / neuron projection maintenance / positive regulation of mitotic nuclear division / Insulin receptor signalling cascade / positive regulation of glycolytic process / positive regulation of cytokine production / positive regulation of long-term synaptic potentiation / endosome lumen / acute-phase response / positive regulation of protein secretion / positive regulation of D-glucose import / insulin receptor binding / positive regulation of cell differentiation / Regulation of insulin secretion / wound healing / negative regulation of protein catabolic process / hormone activity / regulation of synaptic plasticity / positive regulation of neuron projection development / Golgi lumen / positive regulation of protein localization to nucleus / cognition / glucose metabolic process / vasodilation / insulin receptor signaling pathway / glucose homeostasis / cell-cell signaling / regulation of protein localization / PI5P, PP2A and IER3 Regulate PI3K/AKT Signaling / positive regulation of cell growth / protease binding / secretory granule lumen / positive regulation of canonical NF-kappaB signal transduction / positive regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction / positive regulation of MAPK cascade / positive regulation of cell migration / G protein-coupled receptor signaling pathway / endoplasmic reticulum lumen / Amyloid fiber formation / Golgi membrane / negative regulation of gene expression / positive regulation of cell population proliferation / positive regulation of gene expression / regulation of DNA-templated transcription / extracellular space / extracellular region / identical protein binding 類似検索 - 分子機能 | ||||||
手法 | ![]() | ||||||
![]() | Wan, Z. / Zhao, M. / Nakagawa, S. / Jia, W. / Weiss, M.A. | ||||||
![]() | ![]() タイトル: Non-standard insulin design: structure-activity relationships at the periphery of the insulin receptor. 著者: Weiss, M.A. / Wan, Z. / Zhao, M. / Chu, Y.C. / Nakagawa, S.H. / Burke, G.T. / Jia, W. / Hellmich, R. / Katsoyannis, P.G. #1: ![]() タイトル: Diabetes-associated mutations in a beta-cell transcription factor destabilize an antiparallel "mini-zipper" in a dimerization interface 著者: Hua, Q.X. / Zhao, M. / Narayana, N. / Nakagawa, S.H. / Jia, W.H. / Weiss, M.A. #2: ![]() タイトル: The Relationship Between Insulin Bioactivity and Structure in the NH2-terminal A-chain Helix 著者: Olsen, H.B. / Ludvigsen, S. / Kaarsholm, N.C. #3: ![]() タイトル: Mapping the Functional Surface of Insulin by Design: Structure and Function of a Novel A-chain Analogue 著者: Hua, Q.X. / Hu, S.Q. / Frank, B.H. / Jia, W.H. / Chu, Y.C. / Wang, S.H. / Burke, G.T. / Kaysoyannis, P.G. / Weiss, M.A. #4: ![]() タイトル: A Proposed Interaction Model of the Insulin Molecule with its Receptor 著者: Liang, D.C. / Chang, W.R. / Wan, Z.L. / Vijayan, N.M. | ||||||
履歴 |
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Remark 400 | COMPOUND THE CONFORMATIONS OF TWO MONOMERS ARE DIFFERENT AS THE RESULT OF A CHANGE IN CONFORMATION ...COMPOUND THE CONFORMATIONS OF TWO MONOMERS ARE DIFFERENT AS THE RESULT OF A CHANGE IN CONFORMATION OF THE FIRST EIGHT RESIDUES OF THE B-CHAINS. |
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構造の表示
構造ビューア | 分子: ![]() ![]() |
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ダウンロードとリンク
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ダウンロード
PDBx/mmCIF形式 | ![]() | 34.8 KB | 表示 | ![]() |
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PDB形式 | ![]() | 24 KB | 表示 | ![]() |
PDBx/mmJSON形式 | ![]() | ツリー表示 | ![]() | |
その他 | ![]() |
-検証レポート
文書・要旨 | ![]() | 382.8 KB | 表示 | ![]() |
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文書・詳細版 | ![]() | 383.8 KB | 表示 | |
XML形式データ | ![]() | 3.7 KB | 表示 | |
CIF形式データ | ![]() | 5.7 KB | 表示 | |
アーカイブディレクトリ | ![]() ![]() | HTTPS FTP |
-関連構造データ
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リンク
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集合体
登録構造単位 | ![]()
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1 | ![]()
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単位格子 |
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Components on special symmetry positions |
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要素
#1: タンパク質・ペプチド | 分子量: 2382.713 Da / 分子数: 2 / 変異: T8(DAB) / 由来タイプ: 合成 詳細: The protein was chemically synthesized. The sequence is naturally found in Homo sapiens(human). 参照: UniProt: P01308 #2: タンパク質・ペプチド | 分子量: 3433.953 Da / 分子数: 2 / 由来タイプ: 合成 詳細: The protein was chemically synthesized. The sequence is naturally found in Homo sapiens(human). 参照: UniProt: P01308 #3: 化合物 | #4: 水 | ChemComp-HOH / | Has protein modification | Y | |
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-実験情報
-実験
実験 | 手法: ![]() |
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試料調製
結晶 | マシュー密度: 1.91 Å3/Da / 溶媒含有率: 35.75 % | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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結晶化 | 温度: 298 K / 手法: 蒸気拡散法, ハンギングドロップ法 / pH: 6.4 詳細: Tris, phonel, acetone, sodium citrate, pH 6.4, VAPOR DIFFUSION, HANGING DROP, temperature 298K | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||
結晶化 | *PLUS pH: 7.8 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||
溶液の組成 | *PLUS
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-データ収集
回折 | 平均測定温度: 100 K |
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放射光源 | 由来: ![]() |
検出器 | タイプ: RIGAKU RAXIS II / 検出器: IMAGE PLATE / 日付: 1998年4月26日 / 詳細: mirrors |
放射 | モノクロメーター: Mirrors / プロトコル: SINGLE WAVELENGTH / 単色(M)・ラウエ(L): M / 散乱光タイプ: x-ray |
放射波長 | 波長: 1.5418 Å / 相対比: 1 |
反射 | 解像度: 2→10 Å / Num. all: 5681 / Num. obs: 5448 / % possible obs: 95 % / Observed criterion σ(F): 2 / Observed criterion σ(I): 1 / 冗長度: 3 % / Rmerge(I) obs: 0.05 |
反射 シェル | 解像度: 2→2.03 Å / 冗長度: 611 % / Rmerge(I) obs: 0.097 / % possible all: 100 |
反射 | *PLUS % possible obs: 93 % / Rmerge(I) obs: 0.056 |
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解析
ソフトウェア |
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精密化 | 解像度: 2→10 Å / σ(F): 2 / σ(I): 1
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精密化ステップ | サイクル: LAST / 解像度: 2→10 Å
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ソフトウェア | *PLUS 名称: ![]() | |||||||||||||||||||||||||
精密化 | *PLUS 最高解像度: 2 Å / 最低解像度: 10 Å / σ(F): 2 / % reflection Rfree: 9.2 % / Rfactor obs: 0.205 | |||||||||||||||||||||||||
溶媒の処理 | *PLUS | |||||||||||||||||||||||||
原子変位パラメータ | *PLUS | |||||||||||||||||||||||||
拘束条件 | *PLUS
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