+データを開く
-基本情報
登録情報 | データベース: PDB / ID: 1j5n | ||||||
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タイトル | Solution Structure of the Non-Sequence-Specific HMGB protein NHP6A in complex with SRY DNA | ||||||
要素 |
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キーワード | DNA BINDING PROTEIN/DNA / HMG-BOX / HMGB / PROTEIN-DNA COMPLEX / ALPHA HELIX / DOUBLE HELIX / DNA BINDING PROTEIN-DNA COMPLEX | ||||||
機能・相同性 | 機能・相同性情報 maintenance of transcriptional fidelity during transcription elongation by RNA polymerase III / Apoptosis induced DNA fragmentation / Regulation of TLR by endogenous ligand / Mitochondrial transcription initiation / MutSalpha complex binding / protein-DNA complex assembly / Pyroptosis / RNA polymerase III preinitiation complex assembly / Mitochondrial protein degradation / DNA binding, bending ...maintenance of transcriptional fidelity during transcription elongation by RNA polymerase III / Apoptosis induced DNA fragmentation / Regulation of TLR by endogenous ligand / Mitochondrial transcription initiation / MutSalpha complex binding / protein-DNA complex assembly / Pyroptosis / RNA polymerase III preinitiation complex assembly / Mitochondrial protein degradation / DNA binding, bending / negative regulation of DNA binding / nucleosome binding / RNA polymerase II preinitiation complex assembly / Neutrophil degranulation / protein-DNA complex / chromatin organization / chromosome / nucleic acid binding / chromatin remodeling / DNA repair / nucleus 類似検索 - 分子機能 | ||||||
生物種 | Saccharomyces cerevisiae (パン酵母) | ||||||
手法 | 溶液NMR / simulated annealing | ||||||
データ登録者 | Masse, J.E. / Wong, B. / Yen, Y.-M. / Allain, F.H.-T. / Johnson, R.C. / Feigon, J. | ||||||
引用 | ジャーナル: J.Mol.Biol. / 年: 2002 タイトル: The S. cerevisiae architectural HMGB protein NHP6A complexed with DNA: DNA and protein conformational changes upon binding 著者: Masse, J.E. / Wong, B. / Yen, Y.-M. / Allain, F.H.-T. / Johnson, R.C. / Feigon, J. | ||||||
履歴 |
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-構造の表示
構造ビューア | 分子: MolmilJmol/JSmol |
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-ダウンロードとリンク
-ダウンロード
PDBx/mmCIF形式 | 1j5n.cif.gz | 974.6 KB | 表示 | PDBx/mmCIF形式 |
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PDB形式 | pdb1j5n.ent.gz | 804.2 KB | 表示 | PDB形式 |
PDBx/mmJSON形式 | 1j5n.json.gz | ツリー表示 | PDBx/mmJSON形式 | |
その他 | その他のダウンロード |
-検証レポート
文書・要旨 | 1j5n_validation.pdf.gz | 371.8 KB | 表示 | wwPDB検証レポート |
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文書・詳細版 | 1j5n_full_validation.pdf.gz | 779.2 KB | 表示 | |
XML形式データ | 1j5n_validation.xml.gz | 114.2 KB | 表示 | |
CIF形式データ | 1j5n_validation.cif.gz | 148.1 KB | 表示 | |
アーカイブディレクトリ | https://data.pdbj.org/pub/pdb/validation_reports/j5/1j5n ftp://data.pdbj.org/pub/pdb/validation_reports/j5/1j5n | HTTPS FTP |
-関連構造データ
-リンク
-集合体
登録構造単位 |
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NMR アンサンブル |
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-要素
#1: DNA鎖 | 分子量: 4696.042 Da / 分子数: 1 / 由来タイプ: 合成 詳細: unlabeled DNA made on a commercial synthesizer, 15N,13C-labeled DNA made enzymatically in vitro with Taq polymerase |
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#2: DNA鎖 | 分子量: 4482.940 Da / 分子数: 1 / 由来タイプ: 合成 詳細: unlabeled DNA made on a commercial synthesizer, 15N,13C-labeled DNA made enzymatically in vitro with Taq polymerase |
#3: タンパク質 | 分子量: 10824.364 Da / 分子数: 1 / 由来タイプ: 組換発現 由来: (組換発現) Saccharomyces cerevisiae (パン酵母) 発現宿主: Escherichia coli (大腸菌) / 参照: UniProt: P11632 |
-実験情報
-実験
実験 | 手法: 溶液NMR | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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NMR実験 |
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NMR実験の詳細 | Text: This structure calculated using standard 3D heteronuclear filtering and editing techniques with both labeled protein and labeled DNA. |
-試料調製
詳細 |
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試料状態 |
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結晶化 | *PLUS 手法: other / 詳細: NMR |
-NMR測定
NMRスペクトロメーター | タイプ: Bruker DRX / 製造業者: Bruker / モデル: DRX / 磁場強度: 600 MHz |
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-解析
NMR software |
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精密化 | 手法: simulated annealing / ソフトェア番号: 1 詳細: The structures are based on a total of 3035 restraints. 102 are from alpha-helical hydrogen bond restraints within the protein (2/h-bond). 76 are hydrogen bond restraints for Watson-Crick ...詳細: The structures are based on a total of 3035 restraints. 102 are from alpha-helical hydrogen bond restraints within the protein (2/h-bond). 76 are hydrogen bond restraints for Watson-Crick base pairs in the DNA. The rest are NOE-derived. 2197 of the NOE restraints are protein-protein restraints, 578 are DNA-DNA restraints, and 82 are protein-DNA restraints. | ||||||||||||||||
代表構造 | 選択基準: closest to the average | ||||||||||||||||
NMRアンサンブル | コンフォーマー選択の基準: structures with acceptable covalent geometry 計算したコンフォーマーの数: 100 / 登録したコンフォーマーの数: 20 |