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データを開く
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基本情報
登録情報 | データベース: PDB / ID: 1j2o | ||||||
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タイトル | Structure of FLIN2, a complex containing the N-terminal LIM domain of LMO2 and ldb1-LID | ||||||
![]() | Fusion of Rhombotin-2 and LIM domain-binding protein 1 | ||||||
![]() | METAL BINDING PROTEIN / LIM domain / LIM-interaction-domain (LID) | ||||||
機能・相同性 | ![]() regulation of kinase activity / cellular component assembly / negative regulation of erythrocyte differentiation / positive regulation of hemoglobin biosynthetic process / cerebellar Purkinje cell differentiation / epithelial structure maintenance / head development / primitive erythrocyte differentiation / beta-catenin-TCF complex / RUNX1 regulates transcription of genes involved in differentiation of HSCs ...regulation of kinase activity / cellular component assembly / negative regulation of erythrocyte differentiation / positive regulation of hemoglobin biosynthetic process / cerebellar Purkinje cell differentiation / epithelial structure maintenance / head development / primitive erythrocyte differentiation / beta-catenin-TCF complex / RUNX1 regulates transcription of genes involved in differentiation of HSCs / transcription-dependent tethering of RNA polymerase II gene DNA at nuclear periphery / LIM domain binding / gastrulation with mouth forming second / anterior/posterior axis specification / embryonic hemopoiesis / regulation of focal adhesion assembly / cell leading edge / somatic stem cell population maintenance / positive regulation of cell adhesion / hair follicle development / regulation of cell migration / cerebellum development / positive regulation of transcription elongation by RNA polymerase II / neuron differentiation / Wnt signaling pathway / RNA polymerase II transcription regulator complex / : / nervous system development / DNA-binding transcription factor binding / RNA polymerase II-specific DNA-binding transcription factor binding / transcription regulator complex / transcription by RNA polymerase II / transcription coactivator activity / cell adhesion / chromatin binding / chromatin / enzyme binding / negative regulation of transcription by RNA polymerase II / protein homodimerization activity / positive regulation of transcription by RNA polymerase II / protein-containing complex / nucleoplasm / identical protein binding / nucleus / metal ion binding 類似検索 - 分子機能 | ||||||
生物種 | ![]() ![]() | ||||||
手法 | 溶液NMR / distance geometry, simulated annealing, molecular dynamics, automated noe assignment of ambiguous NOES | ||||||
![]() | Deane, J.E. / Mackay, J.P. / Kwan, A.H. / Sum, E.Y. / Visvader, J.E. / Matthews, J.M. | ||||||
![]() | ![]() タイトル: Structural basis for the recognition of ldb1 by the N-terminal LIM domains of LMO2 and LMO4 著者: Deane, J.E. / Mackay, J.P. / Kwan, A.H. / Sum, E.Y. / Visvader, J.E. / Matthews, J.M. #1: ![]() タイトル: Design, production and characterization of FLIN2 and FLIN4: the engineering of intramolecular ldb1:LMO complexes. 著者: Deane, J.E. / Visvader, J.E. / Mackay, J.P. / Matthews, J.M. | ||||||
履歴 |
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構造の表示
構造ビューア | 分子: ![]() ![]() |
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ダウンロードとリンク
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ダウンロード
PDBx/mmCIF形式 | ![]() | 667 KB | 表示 | ![]() |
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PDB形式 | ![]() | 553.5 KB | 表示 | ![]() |
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その他 | ![]() |
-検証レポート
文書・要旨 | ![]() | 346.5 KB | 表示 | ![]() |
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文書・詳細版 | ![]() | 500.9 KB | 表示 | |
XML形式データ | ![]() | 41.5 KB | 表示 | |
CIF形式データ | ![]() | 68.4 KB | 表示 | |
アーカイブディレクトリ | ![]() ![]() | HTTPS FTP |
-関連構造データ
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リンク
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集合体
登録構造単位 | ![]()
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NMR アンサンブル |
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要素
#1: タンパク質 | 分子量: 12604.148 Da / 分子数: 1 / 由来タイプ: 組換発現 詳細: Protein is a fusion of the N-terminal LIM domain of LMO2 (residues 26-87) followed by an eleven residue linker (GGSGGHMGSGG) than the LID domain from ldb1 (residues 300-339). Originally ...詳細: Protein is a fusion of the N-terminal LIM domain of LMO2 (residues 26-87) followed by an eleven residue linker (GGSGGHMGSGG) than the LID domain from ldb1 (residues 300-339). Originally expressed as a fusion with GST. GST portion removed by treatment with thrombin. 由来: (組換発現) ![]() ![]() ![]() ![]() |
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#2: 化合物 |
-実験情報
-実験
実験 | 手法: 溶液NMR | ||||||||||||||||
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NMR実験 |
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NMR実験の詳細 | Text: Backbone and Side-chain assignment made using standard 2D and 3D experiments. |
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試料調製
詳細 |
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試料状態 | pH: 7.0 / 圧: ambient / 温度: 298 K | ||||||||||||
結晶化 | *PLUS 手法: other / 詳細: NMR |
-NMR測定
放射 | プロトコル: SINGLE WAVELENGTH / 単色(M)・ラウエ(L): M |
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放射波長 | 相対比: 1 |
NMRスペクトロメーター | タイプ: Bruker DRX / 製造業者: Bruker / モデル: DRX / 磁場強度: 600 MHz |
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解析
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精密化 | 手法: distance geometry, simulated annealing, molecular dynamics, automated noe assignment of ambiguous NOES ソフトェア番号: 1 詳細: The structures are based on 1690 NOE contraints (including 50 ambiguous constraints) and 134 torsion angle restaints | ||||||||||||||||||||||||||||||||
NMRアンサンブル | コンフォーマー選択の基準: structures with favorable non-bond energy 計算したコンフォーマーの数: 1000 / 登録したコンフォーマーの数: 20 |