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Yorodumi- PDB-1j0t: The solution structure of molt-inhibiting hormone from the kuruma... -
+Open data
-Basic information
Entry | Database: PDB / ID: 1j0t | ||||||
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Title | The solution structure of molt-inhibiting hormone from the kuruma prawn | ||||||
Components | MOLT-INHIBITING HORMONE | ||||||
Keywords | Hormone/growth factor / ALPHA-HELICAL PROTEIN / Hormone-growth factor COMPLEX | ||||||
Function / homology | Function and homology information neuropeptide hormone activity / neuropeptide signaling pathway / extracellular region Similarity search - Function | ||||||
Biological species | Marsupenaeus japonicus (crustacean) | ||||||
Method | SOLUTION NMR / distance geometry torsion angle dynamics | ||||||
Authors | Katayama, H. / Nagata, K. / Ohira, T. / Yumoto, F. / Tanokura, M. / Nagasawa, H. | ||||||
Citation | Journal: J.Biol.Chem. / Year: 2003 Title: The solution structure of molt-inhibiting hormone from the Kuruma prawn Marsupenaeus japonicus Authors: Katayama, H. / Nagata, K. / Ohira, T. / Yumoto, F. / Tanokura, M. / Nagasawa, H. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1j0t.cif.gz | 253.4 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1j0t.ent.gz | 208.3 KB | Display | PDB format |
PDBx/mmJSON format | 1j0t.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/j0/1j0t ftp://data.pdbj.org/pub/pdb/validation_reports/j0/1j0t | HTTPS FTP |
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-Related structure data
Similar structure data |
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-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein | Mass: 9152.621 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Marsupenaeus japonicus (crustacean) / Plasmid: pET28a(+) / Species (production host): Escherichia coli / Production host: Escherichia coli BL21(DE3) (bacteria) / Strain (production host): BL21(DE3) / References: UniProt: P55847 |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||
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NMR experiment |
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NMR details | Text: The structure was determined using triple-resonance NMR spectroscopy. |
-Sample preparation
Details |
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Sample conditions | Ionic strength: 0 / pH: 7.4 / Pressure: ambient / Temperature: 298 K | |||||||||
Crystal grow | *PLUS Method: other / Details: NMR |
-NMR measurement
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M |
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Radiation wavelength | Relative weight: 1 |
NMR spectrometer | Type: Varian INOVA / Manufacturer: Varian / Model: INOVA / Field strength: 500 MHz |
-Processing
NMR software | Name: DYANA / Version: 1.4 / Developer: Guentert / Classification: refinement |
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Refinement | Method: distance geometry torsion angle dynamics / Software ordinal: 1 |
NMR representative | Selection criteria: fewest violations |
NMR ensemble | Conformer selection criteria: back calculated data agree with experimental NOESY spectrum Conformers calculated total number: 50 / Conformers submitted total number: 10 |