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- PDB-1iwn: Crystal Structure of the Outer Membrane Lipoprotein Receptor LolB... -
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Open data
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Basic information
Entry | Database: PDB / ID: 1iwn | ||||||
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Title | Crystal Structure of the Outer Membrane Lipoprotein Receptor LolB Complexed with PEGMME2000 | ||||||
![]() | Outer Membrane Lipoprotein LolB | ||||||
![]() | PROTEIN TRANSPORT / UNCLOSED BETA BARREL / LIPOPROTEIN | ||||||
Function / homology | ![]() lipoprotein localization to outer membrane / lipoprotein metabolic process / cell outer membrane / protein transport / outer membrane-bounded periplasmic space Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Takeda, K. / Miyatake, H. / Yokota, N. / Matsuyama, S. / Tokuda, H. / Miki, K. | ||||||
![]() | ![]() Title: Crystal structures of bacterial lipoprotein localization factors, LolA and LolB. Authors: Takeda, K. / Miyatake, H. / Yokota, N. / Matsuyama, S. / Tokuda, H. / Miki, K. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 52.3 KB | Display | ![]() |
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PDB format | ![]() | 36.8 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 451.4 KB | Display | ![]() |
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Full document | ![]() | 456.2 KB | Display | |
Data in XML | ![]() | 10.8 KB | Display | |
Data in CIF | ![]() | 14.2 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 1iwlC ![]() 1iwmSC ![]() 1ua8C C: citing same article ( S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 21225.721 Da / Num. of mol.: 1 / Mutation: C1A Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() | ||||
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#2: Chemical | #3: Chemical | ChemComp-PG5 / | #4: Water | ChemComp-HOH / | |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.28 Å3/Da / Density % sol: 45.6 % | ||||||||||||||||||||||||||||||||||||||||||
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, sitting drop / pH: 4.6 Details: PEGMME2000, sodium acetate, ammonium sulfate, sodium iodide, pH 4.6, VAPOR DIFFUSION, SITTING DROP, temperature 293K | ||||||||||||||||||||||||||||||||||||||||||
Crystal grow | *PLUS Temperature: 20 ℃ / pH: 8 / Method: vapor diffusion, hanging drop | ||||||||||||||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 90 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: MARRESEARCH / Detector: CCD / Date: Jun 7, 2001 |
Radiation | Monochromator: Si 111 / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.1 Å / Relative weight: 1 |
Reflection | Resolution: 2.2→30 Å / Num. obs: 10647 / % possible obs: 98.2 % / Observed criterion σ(F): 0 / Observed criterion σ(I): 0 / Redundancy: 19.9 % / Biso Wilson estimate: 33.9 Å2 / Rsym value: 0.073 |
Reflection shell | Resolution: 2.2→2.28 Å / Rsym value: 0.253 / % possible all: 93.4 |
Reflection | *PLUS Highest resolution: 2.2 Å / Lowest resolution: 30 Å / Rmerge(I) obs: 0.073 |
Reflection shell | *PLUS % possible obs: 93.4 % / Rmerge(I) obs: 0.253 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: PDB ENTRY 1IWM (chain A) Resolution: 2.2→30 Å / Isotropic thermal model: Isotropic / Cross valid method: THROUGHOUT / σ(F): 0 / Stereochemistry target values: Engh & Huber
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Displacement parameters | Biso mean: 34.4 Å2
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Refine analyze |
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Refinement step | Cycle: LAST / Resolution: 2.2→30 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 2.2→2.34 Å / Rfactor Rfree error: 0.03
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Refinement | *PLUS Highest resolution: 2.2 Å / Lowest resolution: 30 Å / % reflection Rfree: 5 % | ||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||
Displacement parameters | *PLUS | ||||||||||||||||
LS refinement shell | *PLUS Lowest resolution: 2.28 Å |