[English] 日本語
Yorodumi- PDB-1ise: Crystal structure of a mutant of ribosome recycling factor from E... -
+Open data
-Basic information
Entry | Database: PDB / ID: 1ise | ||||||
---|---|---|---|---|---|---|---|
Title | Crystal structure of a mutant of ribosome recycling factor from Escherichia coli, Arg132Gly | ||||||
Components | Ribosome Recycling Factor | ||||||
Keywords | TRANSLATION | ||||||
Function / homology | Function and homology information cytoplasmic translational termination / ribosomal large subunit binding / translation / cytosol / cytoplasm Similarity search - Function | ||||||
Biological species | Escherichia coli (E. coli) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.2 Å | ||||||
Authors | Nakano, H. / Yoshida, T. / Oka, S. / Uchiyama, S. / Nishina, K. / Ohkubo, T. / Kato, H. / Yamagata, Y. / Kobayashi, Y. | ||||||
Citation | Journal: To be Published Title: Crystal structure of a mutant of ribosome recycling factor from Escherichia coli, Arg132Gly Authors: Nakano, H. / Yoshida, T. / Oka, S. / Uchiyama, S. / Nishina, K. / Ohkubo, T. / Kato, H. / Yamagata, Y. / Kobayashi, Y. | ||||||
History |
|
-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
---|
-Downloads & links
-Download
PDBx/mmCIF format | 1ise.cif.gz | 50.7 KB | Display | PDBx/mmCIF format |
---|---|---|---|---|
PDB format | pdb1ise.ent.gz | 36.3 KB | Display | PDB format |
PDBx/mmJSON format | 1ise.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/is/1ise ftp://data.pdbj.org/pub/pdb/validation_reports/is/1ise | HTTPS FTP |
---|
-Related structure data
Related structure data | |
---|---|
Similar structure data |
-Links
-Assembly
Deposited unit |
| ||||||||
---|---|---|---|---|---|---|---|---|---|
1 |
| ||||||||
Unit cell |
|
-Components
#1: Protein | Mass: 20571.479 Da / Num. of mol.: 1 / Mutation: R132G Source method: isolated from a genetically manipulated source Source: (gene. exp.) Escherichia coli (E. coli) / Gene: frr / Plasmid: pET22b(+) / Species (production host): Escherichia coli / Production host: Escherichia coli BL21(DE3) (bacteria) / Strain (production host): BL21(DE3) / References: UniProt: P0A805 |
---|---|
#2: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
---|
-Sample preparation
Crystal | Density Matthews: 2.55 Å3/Da / Density % sol: 51.84 % |
---|---|
Crystal grow | Temperature: 277 K / Method: vapor diffusion, hanging drop / pH: 5.8 Details: 50mM MES-NaOH, PEG 1500, pH 5.8, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
-Data collection
Diffraction | Mean temperature: 100 K |
---|---|
Diffraction source | Source: SYNCHROTRON / Site: Photon Factory / Beamline: BL-6A / Wavelength: 1 Å |
Detector | Type: WEISSENBERG / Detector: DIFFRACTOMETER / Date: Jul 24, 2000 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1 Å / Relative weight: 1 |
Reflection | Resolution: 2.2→40 Å / Num. obs: 10611 / % possible obs: 98.4 % / Observed criterion σ(F): 2 / Observed criterion σ(I): 2 |
Reflection shell | Resolution: 2.2→2.24 Å / % possible all: 93.1 |
-Processing
Software |
| ||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Refinement | Method to determine structure: MOLECULAR REPLACEMENT / Resolution: 2.2→20 Å / σ(F): 2
| ||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.2→20 Å
| ||||||||||||||||||||
Refine LS restraints |
|