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Open data
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Basic information
Entry | Database: PDB / ID: 1eh1 | ||||||
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Title | RIBOSOME RECYCLING FACTOR FROM THERMUS THERMOPHILUS | ||||||
![]() | RIBOSOME RECYCLING FACTOR | ||||||
![]() | RIBOSOME / TRANSLATION / HINGE VARIABILITY | ||||||
Function / homology | ![]() cytoplasmic translational termination / ribosomal large subunit binding / cytoplasm Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() | ||||||
![]() | Toyoda, T. / Tin, O.F. / Ito, K. / Fujiwara, T. / Kumasaka, T. / Yamamoto, M. / Garber, M.B. / Nakamura, Y. | ||||||
![]() | ![]() Title: Crystal structure combined with genetic analysis of the Thermus thermophilus ribosome recycling factor shows that a flexible hinge may act as a functional switch. Authors: Toyoda, T. / Tin, O.F. / Ito, K. / Fujiwara, T. / Kumasaka, T. / Yamamoto, M. / Garber, M.B. / Nakamura, Y. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 48.6 KB | Display | ![]() |
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PDB format | ![]() | 35.7 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 409.6 KB | Display | ![]() |
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Full document | ![]() | 415.4 KB | Display | |
Data in XML | ![]() | 10.6 KB | Display | |
Data in CIF | ![]() | 13.8 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 |
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Unit cell |
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Components
#1: Protein | Mass: 21029.068 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() |
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#2: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 3.23 Å3/Da / Density % sol: 61.9 % | ||||||||||||||||||||
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, hanging drop / pH: 4.9 Details: ammonium sulphate, sodium acetate, MES, pH 4.9, VAPOR DIFFUSION, HANGING DROP, temperature 20K | ||||||||||||||||||||
Crystal grow | *PLUS pH: 4.6 | ||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 110 K |
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Diffraction source | Source: ![]() |
Detector | Type: RIGAKU / Detector: IMAGE PLATE / Date: Jul 30, 1999 / Details: GRAPHITE CRYSTAL |
Radiation | Protocol: MAD / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.5418 Å / Relative weight: 1 |
Reflection | Resolution: 2.6→50 Å / Num. all: 7568 / Num. obs: 28513 / % possible obs: 99.7 % / Observed criterion σ(F): 2 / Observed criterion σ(I): 2 / Redundancy: 3.8 % / Biso Wilson estimate: 63.5 Å2 / Rmerge(I) obs: 0.038 / Net I/σ(I): 17.6 |
Reflection shell | Resolution: 2.6→2.66 Å / Redundancy: 1.7 % / Rmerge(I) obs: 0.234 / Num. unique all: 7568 / % possible all: 98.7 |
Reflection | *PLUS Num. obs: 7568 / Num. measured all: 28513 |
Reflection shell | *PLUS % possible obs: 98.7 % |
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Processing
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Refinement | Method to determine structure: ![]() Stereochemistry target values: RAMACHANDRAN ET AL BBA 359:298 (1974)
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Displacement parameters | Biso mean: 58.02 Å2 | |||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.6→10 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 2.6→2.72 Å / Total num. of bins used: 8
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Software | *PLUS Name: CNS / Version: 1 / Classification: refinement | |||||||||||||||||||||||||
Refinement | *PLUS Highest resolution: 2.6 Å / Lowest resolution: 10 Å / σ(F): 2 / % reflection Rfree: 5.42 % | |||||||||||||||||||||||||
Solvent computation | *PLUS | |||||||||||||||||||||||||
Displacement parameters | *PLUS | |||||||||||||||||||||||||
Refine LS restraints | *PLUS Type: c_angle_deg / Dev ideal: 1.1 |