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- PDB-1irs: IRS-1 PTB DOMAIN COMPLEXED WITH A IL-4 RECEPTOR PHOSPHOPEPTIDE, N... -

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Basic information

Entry
Database: PDB / ID: 1irs
TitleIRS-1 PTB DOMAIN COMPLEXED WITH A IL-4 RECEPTOR PHOSPHOPEPTIDE, NMR, MINIMIZED AVERAGE STRUCTURE
Components
  • IL-4 RECEPTOR PHOSPHOPEPTIDE
  • IRS-1
KeywordsCOMPLEX (SIGNAL TRANSDUCTION/PEPTIDE) / PHOSPHOTYROSINE BINDING DOMAIN (PTB) / COMPLEX / SIGNAL TRANSDUCTION / COMPLEX (SIGNAL TRANSDUCTION-PEPTIDE) / COMPLEX (SIGNAL TRANSDUCTION-PEPTIDE) complex
Function / homology
Function and homology information


interleukin-4 receptor activity / negative regulation of T-helper 1 cell differentiation / T-helper 1 cell differentiation / production of molecular mediator involved in inflammatory response / positive regulation of T-helper 2 cell differentiation / IRS-related events triggered by IGF1R / positive regulation of glucose metabolic process / positive regulation of fatty acid beta-oxidation / interleukin-4-mediated signaling pathway / positive regulation of mast cell degranulation ...interleukin-4 receptor activity / negative regulation of T-helper 1 cell differentiation / T-helper 1 cell differentiation / production of molecular mediator involved in inflammatory response / positive regulation of T-helper 2 cell differentiation / IRS-related events triggered by IGF1R / positive regulation of glucose metabolic process / positive regulation of fatty acid beta-oxidation / interleukin-4-mediated signaling pathway / positive regulation of mast cell degranulation / IRS-mediated signalling / insulin receptor complex / Activated NTRK3 signals through PI3K / transmembrane receptor protein tyrosine kinase adaptor activity / positive regulation of macrophage activation / Signaling by Leptin / cellular response to fatty acid / cytokine receptor activity / Signaling by LTK / PI3K/AKT activation / T-helper 2 cell differentiation / positive regulation of myoblast fusion / Signaling by ALK / positive regulation of immunoglobulin production / IRS activation / defense response to protozoan / immunoglobulin mediated immune response / PI3K Cascade / SOS-mediated signalling / positive regulation of insulin receptor signaling pathway / positive regulation of glycogen biosynthetic process / Signal attenuation / Growth hormone receptor signaling / phosphatidylinositol 3-kinase binding / positive regulation of chemokine production / insulin-like growth factor receptor binding / signaling adaptor activity / phosphotyrosine residue binding / SH2 domain binding / negative regulation of insulin receptor signaling pathway / Interleukin-7 signaling / insulin-like growth factor receptor signaling pathway / protein kinase C binding / positive regulation of D-glucose import / insulin receptor binding / phosphatidylinositol 3-kinase/protein kinase B signal transduction / negative regulation of insulin secretion / response to insulin / caveola / centriolar satellite / cellular response to insulin stimulus / cytokine-mediated signaling pathway / Constitutive Signaling by Aberrant PI3K in Cancer / Signaling by ALK fusions and activated point mutants / insulin receptor signaling pathway / glucose homeostasis / PIP3 activates AKT signaling / signaling receptor complex adaptor activity / positive regulation of cold-induced thermogenesis / PI5P, PP2A and IER3 Regulate PI3K/AKT Signaling / RAF/MAP kinase cascade / Interleukin-4 and Interleukin-13 signaling / positive regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction / receptor complex / immune response / external side of plasma membrane / intracellular membrane-bounded organelle / positive regulation of cell population proliferation / signal transduction / extracellular region / nucleoplasm / nucleus / plasma membrane / cytoplasm / cytosol
Similarity search - Function
Interleukin-4 receptor alpha, N-terminal / Interleukin-4 receptor alpha chain, N-terminal / Insulin receptor substrate / Phosphotyrosine-binding domain (IRS1-like) / IRS-type PTB domain profile. / Short hematopoietin receptor family 1 signature. / Short hematopoietin receptor, family 1, conserved site / Phosphotyrosine-binding domain / IRS-type PTB domain / PTB domain (IRS-1 type) ...Interleukin-4 receptor alpha, N-terminal / Interleukin-4 receptor alpha chain, N-terminal / Insulin receptor substrate / Phosphotyrosine-binding domain (IRS1-like) / IRS-type PTB domain profile. / Short hematopoietin receptor family 1 signature. / Short hematopoietin receptor, family 1, conserved site / Phosphotyrosine-binding domain / IRS-type PTB domain / PTB domain (IRS-1 type) / Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB) / PH-domain like / PH domain / PH domain profile. / Pleckstrin homology domain. / Pleckstrin homology domain / Fibronectin type-III domain profile. / Fibronectin type III / Fibronectin type III superfamily / PH-like domain superfamily / Roll / Immunoglobulin-like fold / Mainly Beta
Similarity search - Domain/homology
Interleukin-4 receptor subunit alpha / Insulin receptor substrate 1
Similarity search - Component
Biological speciesHomo sapiens (human)
MethodSOLUTION NMR
AuthorsZhou, M.-M. / Huang, B. / Olejniczak, E.T. / Meadows, R.P. / Shuker, S.B. / Miyazaki, M. / Trub, T. / Shoelson, S.E. / Feisk, S.W.
Citation
Journal: Nat.Struct.Biol. / Year: 1996
Title: Structural basis for IL-4 receptor phosphopeptide recognition by the IRS-1 PTB domain.
Authors: Zhou, M.M. / Huang, B. / Olejniczak, E.T. / Meadows, R.P. / Shuker, S.B. / Miyazaki, M. / Trub, T. / Shoelson, S.E. / Fesik, S.W.
#1: Journal: J.Biol.Chem. / Year: 1995
Title: Ptb Domains of Irs-1 and Shc Have Distinct But Overlapping Binding Specificities
Authors: Wolf, G. / Trub, T. / Ottinger, E. / Groninga, L. / Lynch, A. / White, M.F. / Miyazaki, M. / Lee, J. / Shoelson, S.E.
#2: Journal: Nature / Year: 1995
Title: Structure and Ligand Recognition of the Phosphotyrosine Binding Domain of Shc
Authors: Zhou, M.M. / Ravichandran, K.S. / Olejniczak, E.F. / Petros, A.M. / Meadows, R.P. / Sattler, M. / Harlan, J.E. / Wade, W.S. / Burakoff, S.J. / Fesik, S.W.
#3: Journal: Mol.Cell.Biol. / Year: 1994
Title: Characterization of an Interaction between Insulin Receptor Substrate 1 and the Insulin Receptor by Using the Two-Hybrid System
Authors: O'Neill, T.J. / Craparo, A. / Gustafson, T.A.
History
DepositionMar 22, 1996Processing site: BNL
Revision 1.0May 15, 1997Provider: repository / Type: Initial release
Revision 1.1Mar 24, 2008Group: Version format compliance
Revision 1.2Jul 13, 2011Group: Version format compliance
Revision 1.3Feb 23, 2022Group: Database references / Derived calculations / Other
Category: database_2 / pdbx_database_status ...database_2 / pdbx_database_status / pdbx_struct_assembly / pdbx_struct_oper_list / struct_conn / struct_ref_seq_dif
Item: _database_2.pdbx_DOI / _database_2.pdbx_database_accession ..._database_2.pdbx_DOI / _database_2.pdbx_database_accession / _pdbx_database_status.process_site / _struct_conn.pdbx_leaving_atom_flag / _struct_conn.ptnr1_auth_comp_id / _struct_conn.ptnr1_auth_seq_id / _struct_conn.ptnr1_label_atom_id / _struct_conn.ptnr1_label_comp_id / _struct_conn.ptnr1_label_seq_id / _struct_conn.ptnr2_auth_comp_id / _struct_conn.ptnr2_auth_seq_id / _struct_conn.ptnr2_label_atom_id / _struct_conn.ptnr2_label_comp_id / _struct_conn.ptnr2_label_seq_id / _struct_ref_seq_dif.details
Revision 1.4Oct 9, 2024Group: Data collection / Structure summary
Category: chem_comp_atom / chem_comp_bond ...chem_comp_atom / chem_comp_bond / pdbx_entry_details / pdbx_modification_feature

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

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Assembly

Deposited unit
A: IRS-1
B: IL-4 RECEPTOR PHOSPHOPEPTIDE


Theoretical massNumber of molelcules
Total (without water)13,8902
Polymers13,8902
Non-polymers00
Water00
1


  • Idetical with deposited unit
  • defined by author
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
NMR ensembles
DataCriteria
Number of conformers (submitted / calculated)1 / -
Representative

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Components

#1: Protein IRS-1 / INSULIN RECEPTOR SUBSTRATE 1


Mass: 12648.737 Da / Num. of mol.: 1 / Fragment: PTB DOMAIN
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Strain: BL21 (DE2) PLYSS / Cell line: BL21 / Gene: PTB DOMAIN OF IRS-1 / Organ: SKELETAL / Plasmid: PET30B / Gene (production host): PTB DOMAIN OF IRS-1 / Production host: Escherichia coli (E. coli) / References: UniProt: P35568
#2: Protein/peptide IL-4 RECEPTOR PHOSPHOPEPTIDE


Mass: 1241.311 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Production host: Escherichia coli (E. coli) / References: UniProt: P24394
Has protein modificationY

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Experimental details

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Experiment

ExperimentMethod: SOLUTION NMR

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Sample preparation

Crystal grow
*PLUS
Method: other / Details: NMR

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Processing

Software
NameClassification
X-PLORmodel building
X-PLORrefinement
X-PLORphasing
NMR softwareName: X-PLOR / Developer: BRUNGER / Classification: refinement
RefinementSoftware ordinal: 1
Details: SET OF IDEAL BOND LENGTHS AND ANGLES USED DURING REFINEMENT: PARALLHDG.PRO IN X-PLOR.
NMR ensembleConformers submitted total number: 1

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