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Yorodumi- PDB-1igs: INDOLE-3-GLYCEROLPHOSPHATE SYNTHASE FROM SULFOLOBUS SOLFATARICUS ... -
+Open data
-Basic information
Entry | Database: PDB / ID: 1igs | ||||||
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Title | INDOLE-3-GLYCEROLPHOSPHATE SYNTHASE FROM SULFOLOBUS SOLFATARICUS AT 2.0 A RESOLUTION | ||||||
Components | INDOLE-3-GLYCEROLPHOSPHATE SYNTHASE | ||||||
Keywords | SYNTHASE / THERMOSTABLE / TIM-BARREL | ||||||
Function / homology | Function and homology information indole-3-glycerol-phosphate synthase / indole-3-glycerol-phosphate synthase activity / phosphoribosylanthranilate isomerase activity / tryptophan biosynthetic process Similarity search - Function | ||||||
Biological species | Sulfolobus solfataricus (archaea) | ||||||
Method | X-RAY DIFFRACTION / Resolution: 2 Å | ||||||
Authors | Hennig, M. / Darimont, B. / Kirschner, K. / Jansonius, J.N. | ||||||
Citation | Journal: Structure / Year: 1995 Title: 2.0 A structure of indole-3-glycerol phosphate synthase from the hyperthermophile Sulfolobus solfataricus: possible determinants of protein stability. Authors: Hennig, M. / Darimont, B. / Sterner, R. / Kirschner, K. / Jansonius, J.N. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1igs.cif.gz | 66.9 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1igs.ent.gz | 49.6 KB | Display | PDB format |
PDBx/mmJSON format | 1igs.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 1igs_validation.pdf.gz | 378.7 KB | Display | wwPDB validaton report |
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Full document | 1igs_full_validation.pdf.gz | 383.2 KB | Display | |
Data in XML | 1igs_validation.xml.gz | 6.8 KB | Display | |
Data in CIF | 1igs_validation.cif.gz | 11.2 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/ig/1igs ftp://data.pdbj.org/pub/pdb/validation_reports/ig/1igs | HTTPS FTP |
-Related structure data
Related structure data | |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 28626.102 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Sulfolobus solfataricus (archaea) / Strain: M4 / Gene: TRPC / Plasmid: REPRESSOR PLASMID PDM / Species (production host): Escherichia coli / Gene (production host): TRPC Production host: Escherichia coli str. K12 substr. W3110 (bacteria) Strain (production host): W3110 / References: UniProt: Q06121 |
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#2: Chemical | ChemComp-PO4 / |
#3: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION |
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-Sample preparation
Crystal | Density Matthews: 3.9 Å3/Da / Density % sol: 68.5 % | ||||||||||||||||||||||||||||||
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Crystal grow | pH: 5 / Details: pH 5. | ||||||||||||||||||||||||||||||
Crystal | *PLUS | ||||||||||||||||||||||||||||||
Crystal grow | *PLUS Temperature: 4 ℃ / pH: 5 / Method: vapor diffusion, hanging drop | ||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 277 K |
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Diffraction source | Wavelength: 1.5418 |
Detector | Type: MARRESEARCH / Detector: IMAGE PLATE / Date: Dec 23, 1993 |
Radiation | Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.5418 Å / Relative weight: 1 |
Reflection | Resolution: 2→15 Å / Num. obs: 29607 / % possible obs: 96 % / Observed criterion σ(I): 0 / Redundancy: 6.9 % / Rmerge(I) obs: 0.073 |
Reflection | *PLUS Num. measured all: 205248 |
-Processing
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Refinement | Resolution: 2→10 Å / σ(F): 0
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Displacement parameters | Biso mean: 25.5 Å2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2→10 Å
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Refine LS restraints |
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