Group: Data collection / カテゴリ: chem_comp_atom / chem_comp_bond
Remark 999
SEQUENCE "His A 81" in DFF40 is the first residue of a C-terminal His6 tag. All other five His ...SEQUENCE "His A 81" in DFF40 is the first residue of a C-terminal His6 tag. All other five His residues are not observable in the experiment. Chain B, is produced as a chimeric protein containing protein G B1 domain (-44 to 11) and DFF45 (12 to 100). The sequence for protein G B1 can also be found in PDB entry 1GB1 as residues 1 to 56. The engineered mutation when using PDB entry 1GB1 numbering, is T2Q.
CHIMERAOFIGGBINDINGPROTEINGANDDNAFRAGMENTATIONFACTOR45 / DFF45 / INHIBITOR OF CAD
分子量: 16100.919 Da / 分子数: 1 断片: B1 DOMAIN OF PROTEIN G FUSED WITH N-TERMINAL DOMAIN (CIDE DOMAIN) OF DFF45 Mutation: T(-43)Q / 由来タイプ: 組換発現 / 由来: (組換発現) Streptococcus sp., Homo sapiens / 属: Streptococcus, Homo / 生物種: , / 株: , / 遺伝子: DFF45 / プラスミド: PET-30A(+) / 生物種 (発現宿主): Escherichia coli / 発現宿主: Escherichia coli BL21(DE3) (大腸菌) / 株 (発現宿主): BL21(DE3) 参照: UniProt: P19909, GenBank: 2065561, UniProt: O00273*PLUS
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実験情報
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実験
実験
手法: 溶液NMR
NMR実験
Conditions-ID
Experiment-ID
Solution-ID
タイプ
9
1
1
3D 13C-separated NOESY
10
2
1
3D 13C-separated NOESY
3
3
1
3D 15N-separated NOESY
4
4
1
3D 15N-separated NOESY
5
5
1
2D NOESY
6
6
1
2D NOESY
NMR実験の詳細
Text: The structure was determined using triple-resonance NMR spectroscopy. Chain B, is produced as a chimeric protein containing protein G B1 domain (-44 to 11) and DFF45 (12 to 100). The residues ...Text: The structure was determined using triple-resonance NMR spectroscopy. Chain B, is produced as a chimeric protein containing protein G B1 domain (-44 to 11) and DFF45 (12 to 100). The residues of the Protein G B1 domain are used as a solubility enhancement tag to improve solubility and stability of target protein. These residues are not included in the structural calculation, thus the coordinates are not deposited.