+Open data
-Basic information
Entry | Database: PDB / ID: 1iar | ||||||
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Title | INTERLEUKIN-4 / RECEPTOR ALPHA CHAIN COMPLEX | ||||||
Components |
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Keywords | CYTOKINE/RECEPTOR / CYTOKINE RECEPTOR / INTERLEUKIN-4 / CYTOKINE-RECEPTOR COMPLEX | ||||||
Function / homology | Function and homology information interleukin-4 receptor activity / production of molecular mediator involved in inflammatory response / interleukin-4 receptor binding / positive regulation of isotype switching to IgE isotypes / negative regulation of T-helper 1 cell differentiation / negative regulation of complement-dependent cytotoxicity / T-helper 1 cell differentiation / positive regulation of cellular respiration / regulation of isotype switching / Interleukin-18 signaling ...interleukin-4 receptor activity / production of molecular mediator involved in inflammatory response / interleukin-4 receptor binding / positive regulation of isotype switching to IgE isotypes / negative regulation of T-helper 1 cell differentiation / negative regulation of complement-dependent cytotoxicity / T-helper 1 cell differentiation / positive regulation of cellular respiration / regulation of isotype switching / Interleukin-18 signaling / negative regulation of neuroinflammatory response / positive regulation of T-helper 2 cell differentiation / negative regulation of epithelial cell migration / positive regulation of T-helper 2 cell cytokine production / dendritic cell differentiation / positive regulation of isotype switching to IgG isotypes / neuroinflammatory response / interleukin-4-mediated signaling pathway / positive regulation of mast cell degranulation / macrophage activation / positive regulation of interleukin-13 production / myeloid dendritic cell differentiation / positive regulation of amyloid-beta clearance / regulation of phosphorylation / type 2 immune response / activation of Janus kinase activity / T-helper 2 cell differentiation / positive regulation of MHC class II biosynthetic process / positive regulation of macrophage activation / cytokine receptor activity / negative regulation of cellular response to transforming growth factor beta stimulus / positive regulation of T cell differentiation / positive regulation of immunoglobulin production / positive regulation of myoblast fusion / positive regulation of ATP biosynthetic process / defense response to protozoan / negative regulation of osteoclast differentiation / positive regulation of interleukin-10 production / positive regulation of macroautophagy / negative regulation of tumor necrosis factor production / centriolar satellite / regulation of immune response / immunoglobulin mediated immune response / negative regulation of endothelial cell apoptotic process / positive regulation of chemokine production / positive regulation of T cell proliferation / positive regulation of B cell proliferation / positive regulation of tyrosine phosphorylation of STAT protein / T cell activation / cholesterol metabolic process / B cell differentiation / cytokine activity / growth factor activity / cytokine-mediated signaling pathway / negative regulation of inflammatory response / positive regulation of receptor-mediated endocytosis / positive regulation of cold-induced thermogenesis / Interleukin-4 and Interleukin-13 signaling / receptor complex / positive regulation of cell migration / immune response / external side of plasma membrane / negative regulation of DNA-templated transcription / positive regulation of cell population proliferation / positive regulation of gene expression / negative regulation of apoptotic process / positive regulation of DNA-templated transcription / negative regulation of transcription by RNA polymerase II / signal transduction / positive regulation of transcription by RNA polymerase II / extracellular space / extracellular region / nucleoplasm / plasma membrane Similarity search - Function | ||||||
Biological species | Homo sapiens (human) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MAD / Resolution: 2.3 Å | ||||||
Authors | Hage, T. / Sebald, W. / Reinemer, P. | ||||||
Citation | Journal: Cell(Cambridge,Mass.) / Year: 1999 Title: Crystal structure of the interleukin-4/receptor alpha chain complex reveals a mosaic binding interface. Authors: Hage, T. / Sebald, W. / Reinemer, P. #1: Journal: Eur.J.Biochem. / Year: 1998 Title: Crystals of a 1:1 complex between human interleukin-4 and the extracellular domain of its receptor alpha chain. Authors: Hage, T. / Reinemer, P. / Sebald, W. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1iar.cif.gz | 80.7 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1iar.ent.gz | 60.6 KB | Display | PDB format |
PDBx/mmJSON format | 1iar.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/ia/1iar ftp://data.pdbj.org/pub/pdb/validation_reports/ia/1iar | HTTPS FTP |
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-Related structure data
Similar structure data |
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-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 14989.248 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Description: RECOMBINANT; / Production host: Escherichia coli (E. coli) / References: UniProt: P05112 |
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#2: Protein | Mass: 23738.562 Da / Num. of mol.: 1 / Fragment: EXTRACELLULAR DOMAIN / Mutation: M1F, C182A Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Description: RECOMBINANT / Production host: Escherichia coli (E. coli) / References: UniProt: P24394 |
#3: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.4 Å3/Da / Density % sol: 49 % | ||||||||||||||||||||||||||||||||||||
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Crystal grow | pH: 8 / Details: pH 8.0 | ||||||||||||||||||||||||||||||||||||
Crystal grow | *PLUS pH: 5 / Method: vapor diffusion, hanging drop | ||||||||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: SYNCHROTRON / Site: MPG/DESY, HAMBURG / Beamline: BW6 / Wavelength: 1.2 |
Detector | Type: MAR scanner 345 mm plate / Detector: IMAGE PLATE / Date: Nov 1, 1997 / Details: DOUBLE-FOCUSSING TOROIDAL MIRROR |
Radiation | Monochromator: SI(111) DOUBLE MONOCHROMATOR / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.2 Å / Relative weight: 1 |
Reflection | Resolution: 2.3→25 Å / Num. obs: 16897 / % possible obs: 99.7 % / Redundancy: 5.8 % / Rsym value: 0.038 / Net I/σ(I): 52 |
Reflection shell | Resolution: 2.3→2.4 Å / Redundancy: 5.2 % / Mean I/σ(I) obs: 20 / Rsym value: 0.067 / % possible all: 99.2 |
Reflection | *PLUS Rmerge(I) obs: 0.038 |
Reflection shell | *PLUS % possible obs: 99.2 % / Rmerge(I) obs: 0.067 |
-Processing
Software |
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Refinement | Method to determine structure: MAD / Resolution: 2.3→25 Å / Cross valid method: THROUGHOUT / σ(F): 3
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Displacement parameters | Biso mean: 20.5 Å2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.3→25 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 2.3→2.35 Å / Total num. of bins used: 15
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Xplor file | Serial no: 1 / Param file: PAR_CSD (ENGH & HUBER) / Topol file: TOP_CSD (ENGH & HUBER) | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Software | *PLUS Name: X-PLOR / Version: 3.8 / Classification: refinement | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints | *PLUS
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