+データを開く
-基本情報
登録情報 | データベース: PDB / ID: 1i8g | ||||||
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タイトル | SOLUTION STRUCTURE OF PIN1 WW DOMAIN COMPLEXED WITH CDC25 PHOSPHOTHREONINE PEPTIDE | ||||||
要素 |
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キーワード | HYDROLASE/ISOMERASE / CELL DIVISION / NUCLEAR PROTEIN / HYDROLASE-ISOMERASE COMPLEX | ||||||
機能・相同性 | 機能・相同性情報 positive regulation of cell cycle G2/M phase transition / cis-trans isomerase activity / phosphothreonine residue binding / negative regulation of cell motility / ubiquitin ligase activator activity / regulation of protein localization to nucleus / GTPase activating protein binding / postsynaptic cytosol / mitogen-activated protein kinase kinase binding / regulation of mitotic nuclear division ...positive regulation of cell cycle G2/M phase transition / cis-trans isomerase activity / phosphothreonine residue binding / negative regulation of cell motility / ubiquitin ligase activator activity / regulation of protein localization to nucleus / GTPase activating protein binding / postsynaptic cytosol / mitogen-activated protein kinase kinase binding / regulation of mitotic nuclear division / negative regulation of SMAD protein signal transduction / PI5P Regulates TP53 Acetylation / negative regulation of amyloid-beta formation / cytoskeletal motor activity / phosphoserine residue binding / RHO GTPases Activate NADPH Oxidases / protein peptidyl-prolyl isomerization / positive regulation of protein dephosphorylation / protein-tyrosine-phosphatase / ciliary basal body / positive regulation of GTPase activity / regulation of cytokinesis / negative regulation of protein binding / peptidylprolyl isomerase / protein tyrosine phosphatase activity / peptidyl-prolyl cis-trans isomerase activity / Negative regulators of DDX58/IFIH1 signaling / phosphoprotein binding / synapse organization / negative regulation of transforming growth factor beta receptor signaling pathway / regulation of protein phosphorylation / regulation of protein stability / tau protein binding / neuron differentiation / negative regulation of protein catabolic process / negative regulation of ERK1 and ERK2 cascade / ISG15 antiviral mechanism / beta-catenin binding / positive regulation of canonical Wnt signaling pathway / positive regulation of protein binding / midbody / regulation of gene expression / Regulation of TP53 Activity through Phosphorylation / protein stabilization / response to hypoxia / nuclear speck / positive regulation of protein phosphorylation / cell cycle / cell division / glutamatergic synapse / positive regulation of transcription by RNA polymerase II / nucleoplasm / nucleus / cytoplasm / cytosol 類似検索 - 分子機能 | ||||||
手法 | 溶液NMR / distance geometry simulated annealing | ||||||
データ登録者 | Wintjens, R. / Wieruszeski, J.-M. / Drobecq, H. / Lippens, G. / Landrieu, I. | ||||||
引用 | ジャーナル: J.Biol.Chem. / 年: 2001 タイトル: 1H NMR study on the binding of Pin1 Trp-Trp domain with phosphothreonine peptides. 著者: Wintjens, R. / Wieruszeski, J.M. / Drobecq, H. / Rousselot-Pailley, P. / Buee, L. / Lippens, G. / Landrieu, I. | ||||||
履歴 |
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-構造の表示
構造ビューア | 分子: MolmilJmol/JSmol |
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-ダウンロードとリンク
-ダウンロード
PDBx/mmCIF形式 | 1i8g.cif.gz | 158.4 KB | 表示 | PDBx/mmCIF形式 |
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PDB形式 | pdb1i8g.ent.gz | 137 KB | 表示 | PDB形式 |
PDBx/mmJSON形式 | 1i8g.json.gz | ツリー表示 | PDBx/mmJSON形式 | |
その他 | その他のダウンロード |
-検証レポート
文書・要旨 | 1i8g_validation.pdf.gz | 366.8 KB | 表示 | wwPDB検証レポート |
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文書・詳細版 | 1i8g_full_validation.pdf.gz | 460.2 KB | 表示 | |
XML形式データ | 1i8g_validation.xml.gz | 11.8 KB | 表示 | |
CIF形式データ | 1i8g_validation.cif.gz | 18.1 KB | 表示 | |
アーカイブディレクトリ | https://data.pdbj.org/pub/pdb/validation_reports/i8/1i8g ftp://data.pdbj.org/pub/pdb/validation_reports/i8/1i8g | HTTPS FTP |
-関連構造データ
-リンク
-集合体
登録構造単位 |
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NMR アンサンブル |
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-要素
#1: タンパク質・ペプチド | 分子量: 1192.209 Da / 分子数: 1 / 断片: RESIDUES 63-72 / 由来タイプ: 合成 詳細: The ligand phosphopeptide was synthesized from Rink amide resin using the Fmoc strategy and activation by HBTU and HOBT in a 431A peptide synthesizer. The sequence of the peptide is naturally ...詳細: The ligand phosphopeptide was synthesized from Rink amide resin using the Fmoc strategy and activation by HBTU and HOBT in a 431A peptide synthesizer. The sequence of the peptide is naturally found in Xenopus laevis (African clawed frog). 参照: UniProt: P30311, protein-tyrosine-phosphatase |
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#2: タンパク質・ペプチド | 分子量: 4462.899 Da / 分子数: 1 / 断片: WW DOMAIN (RESIDUES 6-44) / 由来タイプ: 合成 詳細: The Pin1 WW domain was obtained by peptide synthesis using the BOC-benzyl strategy and the HBTU in situ activation protocol on an Applied 430A peptide synthesizer. The sequence of the peptide ...詳細: The Pin1 WW domain was obtained by peptide synthesis using the BOC-benzyl strategy and the HBTU in situ activation protocol on an Applied 430A peptide synthesizer. The sequence of the peptide is naturally found in Homo sapiens (Human). 参照: UniProt: Q13526, peptidylprolyl isomerase |
-実験情報
-実験
実験 | 手法: 溶液NMR |
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NMR実験 | タイプ: 2D NOESY |
-試料調製
詳細 | 内容: sample of 1mM WW domain / 4.5 mM Cdc25 ligand buffer of 50 mM deutered Tris-D2O, pH 6.4, 100 mM NaCl 溶媒系: 90% H2O/10% D2O |
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試料状態 | イオン強度: 100 mM NaCl / pH: 6.4 / 圧: ambient / 温度: 285 K |
結晶化 | *PLUS 手法: other / 詳細: NMR |
-NMR測定
NMRスペクトロメーター | タイプ: Bruker DMX / 製造業者: Bruker / モデル: DMX / 磁場強度: 600 MHz |
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-解析
NMR software |
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精密化 | 手法: distance geometry simulated annealing / ソフトェア番号: 1 詳細: hybrid of distance geometry / simulated annealing protocol Minimization procedure using CVFF as force field | ||||||||||||
代表構造 | 選択基準: closest to the average | ||||||||||||
NMRアンサンブル | コンフォーマー選択の基準: structures with the lowest energy 計算したコンフォーマーの数: 50 / 登録したコンフォーマーの数: 10 |