+Open data
-Basic information
Entry | Database: PDB / ID: 2ln8 | ||||||
---|---|---|---|---|---|---|---|
Title | The solution structure of theromacin | ||||||
Components | Theromacin | ||||||
Keywords | ANTIMICROBIAL PROTEIN / antimicrobial peptide | ||||||
Function / homology | Function and homology information | ||||||
Biological species | Hirudo medicinalis (medicinal leech) | ||||||
Method | SOLUTION NMR / simulated annealing | ||||||
Model details | lowest energy, model 1 | ||||||
Authors | Jung, S. / Soennichsen, F.D. / Hung, C.-W. / Tholey, A. / Boidin-Wichlacz, C. / Hausgen, W. / Gelhaus, C. / Desel, C. / Podschun, R. / Watzig, V. ...Jung, S. / Soennichsen, F.D. / Hung, C.-W. / Tholey, A. / Boidin-Wichlacz, C. / Hausgen, W. / Gelhaus, C. / Desel, C. / Podschun, R. / Watzig, V. / Tasiemski, A. / Leippe, M. / Groetzinger, J. | ||||||
Citation | Journal: J.Biol.Chem. / Year: 2012 Title: Macin family of antimicrobial proteins combines antimicrobial and nerve repair activities. Authors: Jung, S. / Sonnichsen, F.D. / Hung, C.W. / Tholey, A. / Boidin-Wichlacz, C. / Haeusgen, W. / Gelhaus, C. / Desel, C. / Podschun, R. / Waetzig, V. / Tasiemski, A. / Leippe, M. / Grotzinger, J. | ||||||
History |
|
-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
---|
-Downloads & links
-Download
PDBx/mmCIF format | 2ln8.cif.gz | 134.9 KB | Display | PDBx/mmCIF format |
---|---|---|---|---|
PDB format | pdb2ln8.ent.gz | 113.3 KB | Display | PDB format |
PDBx/mmJSON format | 2ln8.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/ln/2ln8 ftp://data.pdbj.org/pub/pdb/validation_reports/ln/2ln8 | HTTPS FTP |
---|
-Related structure data
Similar structure data | |
---|---|
Other databases |
-Links
-Assembly
Deposited unit |
| |||||||||
---|---|---|---|---|---|---|---|---|---|---|
1 |
| |||||||||
NMR ensembles |
|
-Components
#1: Protein | Mass: 8465.528 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Hirudo medicinalis (medicinal leech) / Production host: Escherichia coli (E. coli) / References: UniProt: A8I0L8 |
---|
-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR / Details: The solution structure of Thermomacin | ||||||||||||||||||||||||||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
NMR experiment |
|
-Sample preparation
Details | Contents: 1 mM [U-99% 13C; U-99% 15N] protein, 93% H2O/7% D2O Solvent system: 93% H2O/7% D2O |
---|---|
Sample | Conc.: 1 mM / Component: entity-1 / Isotopic labeling: [U-99% 13C; U-99% 15N] |
Sample conditions | Ionic strength: 0.02 / pH: 7.4 / Pressure: ambient / Temperature: 300 K |
-NMR measurement
NMR spectrometer | Type: Bruker Avance / Manufacturer: Bruker / Model: AVANCE / Field strength: 600 MHz |
---|
-Processing
NMR software |
|
---|