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データを開く
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基本情報
登録情報 | データベース: PDB / ID: 1i31 | ||||||
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タイトル | MU2 ADAPTIN SUBUNIT (AP50) OF AP2 CLATHRIN ADAPTOR, COMPLEXED WITH EGFR INTERNALIZATION PEPTIDE FYRALM AT 2.5 A RESOLUTION | ||||||
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![]() | ENDOCYTOSIS/EXOCYTOSIS / beta-sandwich / peptide-binding site / protein-peptide complex / clathrin adaptor / ENDOCYTOSIS-EXOCYTOSIS COMPLEX | ||||||
機能・相同性 | ![]() diterpenoid metabolic process / positive regulation of prolactin secretion / response to hydroxyisoflavone / ovulation cycle / positive regulation of mucus secretion / Gap junction degradation / Formation of annular gap junctions / WNT5A-dependent internalization of FZD2, FZD5 and ROR2 / LDL clearance / tongue development ...diterpenoid metabolic process / positive regulation of prolactin secretion / response to hydroxyisoflavone / ovulation cycle / positive regulation of mucus secretion / Gap junction degradation / Formation of annular gap junctions / WNT5A-dependent internalization of FZD2, FZD5 and ROR2 / LDL clearance / tongue development / Retrograde neurotrophin signalling / VLDLR internalisation and degradation / WNT5A-dependent internalization of FZD4 / midgut development / extrinsic component of presynaptic endocytic zone membrane / MHC class II antigen presentation / hydrogen peroxide metabolic process / regulation of vesicle size / AP-2 adaptor complex / postsynaptic neurotransmitter receptor internalization / Recycling pathway of L1 / response to cobalamin / positive regulation of synaptic vesicle endocytosis / Cargo recognition for clathrin-mediated endocytosis / clathrin adaptor activity / Clathrin-mediated endocytosis / vesicle budding from membrane / clathrin-dependent endocytosis / signal sequence binding / positive regulation of bone resorption / negative regulation of mitotic cell cycle / multivesicular body, internal vesicle lumen / negative regulation of cardiocyte differentiation / epidermal growth factor receptor activity / epidermal growth factor binding / response to UV-A / low-density lipoprotein particle receptor binding / ERBB2-EGFR signaling pathway / morphogenesis of an epithelial fold / response to lipid / digestive tract morphogenesis / positive regulation of vasoconstriction / intracellular vesicle / negative regulation of epidermal growth factor receptor signaling pathway / eyelid development in camera-type eye / cerebral cortex cell migration / Trafficking of GluR2-containing AMPA receptors / protein tyrosine kinase activator activity / positive regulation of receptor internalization / positive regulation of glial cell proliferation / positive regulation of phosphorylation / endocytic vesicle / synaptic vesicle endocytosis / epidermis development / hair follicle development / cellular response to dexamethasone stimulus / positive regulation of G1/S transition of mitotic cell cycle / embryonic placenta development / negative regulation of protein localization to plasma membrane / positive regulation of synaptic transmission, glutamatergic / salivary gland morphogenesis / positive regulation of superoxide anion generation / neuron projection morphogenesis / clathrin-coated pit / transmembrane receptor protein tyrosine kinase activity / liver regeneration / astrocyte activation / positive regulation of smooth muscle cell proliferation / receptor-mediated endocytosis / lung development / positive regulation of DNA repair / basal plasma membrane / synaptic membrane / positive regulation of DNA replication / epithelial cell proliferation / positive regulation of epithelial cell proliferation / positive regulation of protein localization to plasma membrane / cellular response to amino acid stimulus / intracellular protein transport / phosphatidylinositol 3-kinase/protein kinase B signal transduction / cellular response to estradiol stimulus / liver development / cellular response to mechanical stimulus / cell-cell adhesion / circadian rhythm / receptor protein-tyrosine kinase / response to calcium ion / negative regulation of protein catabolic process / cellular response to growth factor stimulus / receptor internalization / positive regulation of miRNA transcription / kinase binding / vasodilation / ruffle membrane / integrin binding / cellular response to xenobiotic stimulus / epidermal growth factor receptor signaling pathway / cell morphogenesis / terminal bouton / neuron differentiation 類似検索 - 分子機能 | ||||||
生物種 | ![]() ![]() | ||||||
手法 | ![]() ![]() ![]() | ||||||
![]() | Modis, Y. / Boll, W. / Rapoport, I. / Kirchhausen, T. | ||||||
![]() | ![]() タイトル: MU2 ADAPTIN SUBUNIT (AP50) OF AP2 CLATHRIN ADAPTOR, COMPLEXED WITH EGFR INTERNALIZATION PEPTIDE FYRALM AT 2.5 A RESOLUTION 著者: Modis, Y. / Boll, W. / Rapoport, I. / Kirchhausen, T. #1: ![]() タイトル: A Structural Explanation for the Recognition of Tyrosine-Based Endocytic Signals 著者: Owen, D.J. / Evans, P.R. | ||||||
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構造の表示
構造ビューア | 分子: ![]() ![]() |
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ダウンロードとリンク
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ダウンロード
PDBx/mmCIF形式 | ![]() | 70.1 KB | 表示 | ![]() |
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PDB形式 | ![]() | 50.5 KB | 表示 | ![]() |
PDBx/mmJSON形式 | ![]() | ツリー表示 | ![]() | |
その他 | ![]() |
-検証レポート
文書・要旨 | ![]() | 438.8 KB | 表示 | ![]() |
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文書・詳細版 | ![]() | 459.7 KB | 表示 | |
XML形式データ | ![]() | 15.1 KB | 表示 | |
CIF形式データ | ![]() | 19.7 KB | 表示 | |
アーカイブディレクトリ | ![]() ![]() | HTTPS FTP |
-関連構造データ
関連構造データ | ![]() 1bw8S S: 精密化の開始モデル |
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類似構造データ |
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リンク
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集合体
登録構造単位 | ![]()
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単位格子 |
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Components on special symmetry positions |
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要素
#1: タンパク質 | 分子量: 35746.543 Da / 分子数: 1 / 断片: RESIDUES 122-435 / 由来タイプ: 組換発現 / 由来: (組換発現) ![]() ![]() ![]() ![]() |
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#2: タンパク質・ペプチド | 分子量: 800.988 Da / 分子数: 1 / 断片: RESIDUES 998-1003 / 由来タイプ: 合成 詳細: The hexapeptide of epidermal growth factor receptor was chemically synthesized 参照: GenBank: 6478868, UniProt: A8IP97*PLUS |
#3: 水 | ChemComp-HOH / |
-実験情報
-実験
実験 | 手法: ![]() |
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試料調製
結晶 | マシュー密度: 4.66 Å3/Da / 溶媒含有率: 73.59 % |
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結晶化 | 温度: 291 K / 手法: 蒸気拡散法, ハンギングドロップ法 / pH: 4.5 詳細: SODIUM FORMATE, SODIUM ACETATE, pH 4.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
-データ収集
回折 | 平均測定温度: 100 K |
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放射光源 | 由来: ![]() ![]() ![]() |
検出器 | タイプ: ADSC QUANTUM 4 / 検出器: CCD / 日付: 2000年6月14日 / 詳細: BENT CYLINDRICAL SI-MIRROR (RH COATING) |
放射 | モノクロメーター: SI(111) DOUBLE CRYSTAL MONOCHROMATOR プロトコル: SINGLE WAVELENGTH / 単色(M)・ラウエ(L): M / 散乱光タイプ: x-ray |
放射波長 | 波長: 1.006 Å / 相対比: 1 |
反射 | 解像度: 2.5→20 Å / Num. all: 21919 / Num. obs: 21919 / % possible obs: 96.4 % / Observed criterion σ(F): 0 / Observed criterion σ(I): 0 / 冗長度: 50.8 % / Biso Wilson estimate: 74.6 Å2 / Rmerge(I) obs: 0.077 / Net I/σ(I): 25.8 |
反射 シェル | 解像度: 2.52→2.56 Å / 冗長度: 6 % / Rmerge(I) obs: 0.599 / Mean I/σ(I) obs: 1.6 / % possible all: 76.2 |
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解析
ソフトウェア |
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精密化 | 構造決定の手法: ![]() 開始モデル: PDB ENTRY 1BW8 解像度: 2.5→20 Å Isotropic thermal model: ISOTROPIC ATOMIC TEMPERATURE FACTORS 交差検証法: THROUGHOUT / σ(F): 0 / σ(I): 0 / 立体化学のターゲット値: ENGH & HUBER 詳細: MINIMIZATION OF MAXIMUM LIKELIHOOD RESIDUAL BY CONJUGATE DIRECTION METHOD
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原子変位パラメータ | Biso mean: -0.01087 Å2
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Refine analyze |
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精密化ステップ | サイクル: LAST / 解像度: 2.5→20 Å
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拘束条件 |
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LS精密化 シェル |
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