CELL CYCLE / beta-propeller / G fold or GTPase fold
Function / homology
Function and homology information
mitotic nuclear membrane reassembly / RNA nuclear export complex / pre-miRNA export from nucleus / snRNA import into nucleus / cellular response to mineralocorticoid stimulus / manchette / sulfate binding / Regulation of cholesterol biosynthesis by SREBP (SREBF) / importin-alpha family protein binding / protein localization to nucleolus ...mitotic nuclear membrane reassembly / RNA nuclear export complex / pre-miRNA export from nucleus / snRNA import into nucleus / cellular response to mineralocorticoid stimulus / manchette / sulfate binding / Regulation of cholesterol biosynthesis by SREBP (SREBF) / importin-alpha family protein binding / protein localization to nucleolus / Rev-mediated nuclear export of HIV RNA / Nuclear import of Rev protein / GTP metabolic process / NEP/NS2 Interacts with the Cellular Export Machinery / tRNA processing in the nucleus / Postmitotic nuclear pore complex (NPC) reformation / MicroRNA (miRNA) biogenesis / regulation of mitotic nuclear division / DNA metabolic process / dynein intermediate chain binding / mitotic sister chromatid segregation / spermatid development / ribosomal large subunit export from nucleus / sperm flagellum / spindle assembly / ribosomal small subunit export from nucleus / ribosomal subunit export from nucleus / nuclear pore / nucleosome binding / nucleosomal DNA binding / centriole / protein export from nucleus / viral process / guanyl-nucleotide exchange factor activity / mitotic spindle organization / G protein activity / condensed nuclear chromosome / male germ cell nucleus / chromosome segregation / hippocampus development / Transcriptional regulation by small RNAs / Hydrolases; Acting on acid anhydrides; Acting on GTP to facilitate cellular and subcellular movement / recycling endosome / small GTPase binding / positive regulation of protein import into nucleus / G1/S transition of mitotic cell cycle / protein import into nucleus / GDP binding / melanosome / positive regulation of protein binding / nuclear envelope / chromosome / mitotic cell cycle / midbody / histone binding / actin cytoskeleton organization / cadherin binding / protein heterodimerization activity / protein domain specific binding / cell division / GTPase activity / chromatin binding / chromatin / nucleolus / GTP binding / magnesium ion binding / protein-containing complex / RNA binding / extracellular exosome / nucleoplasm / membrane / nucleus / cytoplasm / cytosol Similarity search - Function
Regulator of chromosome condensation (RCC1) signature 1. / Regulator of chromosome condensation 1/beta-lactamase-inhibitor protein II / Regulator of chromosome condensation (RCC1) signature 2. / Regulator of chromosome condensation (RCC1) repeat / Regulator of chromosome condensation, RCC1 / Regulator of chromosome condensation (RCC1) repeat profile. / Regulator of chromosome condensation 1/beta-lactamase-inhibitor protein II / small GTPase Ran family profile. / Ran GTPase / Ran (Ras-related nuclear proteins) /TC4 subfamily of small GTPases ...Regulator of chromosome condensation (RCC1) signature 1. / Regulator of chromosome condensation 1/beta-lactamase-inhibitor protein II / Regulator of chromosome condensation (RCC1) signature 2. / Regulator of chromosome condensation (RCC1) repeat / Regulator of chromosome condensation, RCC1 / Regulator of chromosome condensation (RCC1) repeat profile. / Regulator of chromosome condensation 1/beta-lactamase-inhibitor protein II / small GTPase Ran family profile. / Ran GTPase / Ran (Ras-related nuclear proteins) /TC4 subfamily of small GTPases / 7 Propeller / Methylamine Dehydrogenase; Chain H / Rho (Ras homology) subfamily of Ras-like small GTPases / Ras subfamily of RAS small GTPases / Small GTPase / Ras family / Rab subfamily of small GTPases / Small GTP-binding protein domain / P-loop containing nucleotide triphosphate hydrolases / P-loop containing nucleoside triphosphate hydrolase / Rossmann fold / 3-Layer(aba) Sandwich / Mainly Beta / Alpha Beta Similarity search - Domain/homology
A: GTP-BINDING NUCLEAR PROTEIN RAN B: REGULATOR OF CHROMOSOME CONDENSATION 1 C: GTP-BINDING NUCLEAR PROTEIN RAN D: REGULATOR OF CHROMOSOME CONDENSATION 1 hetero molecules
Mass: 24456.105 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Plasmid: PGEX-2T / Species (production host): Escherichia coli / Production host: Escherichia coli BL21 (bacteria) / Strain (production host): BL21 / References: UniProt: P62826
#2: Protein
REGULATOROFCHROMOSOMECONDENSATION1 / RCC1
Mass: 42917.402 Da / Num. of mol.: 2 Fragment: PROTEIN TRUNCATED FROM THE N-TERMINAL RESIDUES 1 TO 19 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Plasmid: PTAC / Production host: Escherichia coli (E. coli) / Strain (production host): CK600K / References: UniProt: P18754
Mass: 18.015 Da / Num. of mol.: 475 / Source method: isolated from a natural source / Formula: H2O
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Experimental details
-
Experiment
Experiment
Method: X-RAY DIFFRACTION / Number of used crystals: 4
-
Sample preparation
Crystal
Density Matthews: 1.96 Å3/Da / Density % sol: 37.37 %
Crystal grow
Temperature: 293 K / Method: vapor diffusion, hanging drop / pH: 6.5 Details: 20-10% PEG 4000, 200 mM potassium fluoride, 25 mM potassium phosphate, 25 mM ammonium sulfate, 2 mM EDTA, 3mM DTE, pH 6.5, VAPOR DIFFUSION, HANGING DROP
Crystal grow
*PLUS
Temperature: 20 ℃
Components of the solutions
*PLUS
ID
Conc.
Common name
Crystal-ID
Sol-ID
1
20 %
PEG3350
1
reservoir
2
200mM
KF
1
reservoir
-
Data collection
Diffraction
ID
Mean temperature (K)
Crystal-ID
1
100
1
2
100
1
Diffraction source
Source
Site
Beamline
ID
Wavelength (Å)
SYNCHROTRON
ESRF
BM30A
1
0.9801
SYNCHROTRON
ESRF
ID2
2
0.9903
Detector
Type
ID
Detector
Date
Details
MARRESEARCH
1
IMAGE PLATE
Jun 26, 1999
mirrors
MARRESEARCH
2
IMAGE PLATE
Jul 14, 1999
mirrors
Radiation
ID
Monochromator
Protocol
Monochromatic (M) / Laue (L)
Scattering type
Wavelength-ID
1
Si111and311singlecrystals
SINGLEWAVELENGTH
M
x-ray
1
2
Si111CHANNEL
SINGLEWAVELENGTH
M
x-ray
1
Radiation wavelength
ID
Wavelength (Å)
Relative weight
1
0.9801
1
2
0.9903
1
Reflection
Resolution: 1.63→19.79 Å / Num. all: 123475 / Num. obs: 484600 / % possible obs: 92.3 % / Observed criterion σ(F): 0 / Redundancy: 4.1 % / Biso Wilson estimate: 23.7 Å2 / Limit h max: 30 / Limit h min: -30 / Limit k max: 42 / Limit k min: -30 / Limit l max: 47 / Limit l min: 0 / Observed criterion F max: 156076284.73 / Observed criterion F min: 1060.6 / Rmerge(I) obs: 0.146 / Net I/σ(I): 6.8
Reflection shell
Resolution: 1.63→1.67 Å / Redundancy: 1.5 % / Rmerge(I) obs: 0.316 / Mean I/σ(I) obs: 0.8 / % possible all: 56.1
Reflection shell
*PLUS
% possible obs: 56.1 %
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Processing
Software
Name
Version
Classification
NB
CNS
1
refinement
XDS
datareduction
XDS
datascaling
AMoRE
phasing
Refinement
Method to determine structure: MOLECULAR REPLACEMENT Starting model: PDB ENTRY 1A12 (RCC1) and human Ran-GDP-Mg2+ structure (Scheffzek, K. et al. (1995))
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