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Open data
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Basic information
Entry | Database: PDB / ID: 1a12 | ||||||
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Title | REGULATOR OF CHROMOSOME CONDENSATION (RCC1) OF HUMAN | ||||||
![]() | REGULATOR OF CHROMOSOME CONDENSATION 1 | ||||||
![]() | GUANINE NUCLEOTIDE EXCHANGE FACTOR / GEF / RAN / RAS-LIKE NUCLEAR GTP BINDING PROTEIN | ||||||
Function / homology | ![]() mitotic nuclear membrane reassembly / sulfate binding / Rev-mediated nuclear export of HIV RNA / Nuclear import of Rev protein / Postmitotic nuclear pore complex (NPC) reformation / regulation of mitotic nuclear division / spindle assembly / nucleosome binding / nucleosomal DNA binding / viral process ...mitotic nuclear membrane reassembly / sulfate binding / Rev-mediated nuclear export of HIV RNA / Nuclear import of Rev protein / Postmitotic nuclear pore complex (NPC) reformation / regulation of mitotic nuclear division / spindle assembly / nucleosome binding / nucleosomal DNA binding / viral process / guanyl-nucleotide exchange factor activity / mitotic spindle organization / condensed nuclear chromosome / chromosome segregation / small GTPase binding / G1/S transition of mitotic cell cycle / chromosome / histone binding / protein heterodimerization activity / cell division / chromatin binding / chromatin / protein-containing complex / nucleoplasm / nucleus / cytoplasm Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() | ||||||
![]() | Renault, L. / Nassar, N. / Vetter, I. / Becker, J. / Roth, M. / Wittinghofer, A. | ||||||
![]() | ![]() Title: The 1.7 A crystal structure of the regulator of chromosome condensation (RCC1) reveals a seven-bladed propeller. Authors: Renault, L. / Nassar, N. / Vetter, I. / Becker, J. / Klebe, C. / Roth, M. / Wittinghofer, A. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 248.2 KB | Display | ![]() |
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PDB format | ![]() | 198.4 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 384.1 KB | Display | ![]() |
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Full document | ![]() | 400.1 KB | Display | |
Data in XML | ![]() | 24.7 KB | Display | |
Data in CIF | ![]() | 43 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 |
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Unit cell |
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Noncrystallographic symmetry (NCS) | NCS oper:
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Components
#1: Protein | Mass: 44109.777 Da / Num. of mol.: 3 / Fragment: FULL LENGTH Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() #2: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.19 Å3/Da / Density % sol: 41 % Description: NATIVE DATA SET OBTAINED BY MERGING 3 NATIVE DATA SETS, TWO COLLECTED ON ROTATING ANODE AND ONE COLLECTED AT DESY. |
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Crystal grow | pH: 6.9 / Details: pH 6.9 |
Crystal grow | *PLUS Method: unknown |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Date: May 1, 1997 |
Radiation | Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.9096 Å / Relative weight: 1 |
Reflection | Resolution: 1.7→41.5 Å / Num. obs: 113006 / % possible obs: 94.3 % / Observed criterion σ(I): 0 / Redundancy: 5.3 % / Biso Wilson estimate: 10.2 Å2 / Rmerge(I) obs: 0.083 / Rsym value: 0.083 / Net I/σ(I): 23.1 |
Reflection shell | Resolution: 1.7→1.73 Å / Redundancy: 1.5 % / Rmerge(I) obs: 0.257 / Mean I/σ(I) obs: 2.8 / Rsym value: 0.257 / % possible all: 69.9 |
Reflection | *PLUS Num. measured all: 596594 |
Reflection shell | *PLUS % possible obs: 69.9 % |
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Processing
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Refinement | Method to determine structure: SINGLE ISOMORPHOUS REPLACEMENT (SIR) COMBINED WITH 3-FOLD NCS AVERAGING Resolution: 1.7→41 Å / Rfactor Rfree error: 0.002 / Data cutoff high absF: 1000000 / Data cutoff low absF: 750.001 / Isotropic thermal model: RESTRAINED / Cross valid method: THROUGHOUT / σ(F): 0
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Displacement parameters | Biso mean: 17.2 Å2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine analyze |
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Refinement step | Cycle: LAST / Resolution: 1.7→41 Å
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Refine LS restraints |
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Refine LS restraints NCS | NCS model details: RESTRAINTS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
LS refinement shell | Resolution: 1.7→1.73 Å / Rfactor Rfree error: 0.017 / Total num. of bins used: 20
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Xplor file |
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Software | *PLUS Name: ![]() | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints | *PLUS
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LS refinement shell | *PLUS Rfactor obs: 0.264 |