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Open data
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Basic information
Entry | Database: PDB / ID: 1aw9 | ||||||
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Title | STRUCTURE OF GLUTATHIONE S-TRANSFERASE III IN APO FORM | ||||||
![]() | GLUTATHIONE S-TRANSFERASE III | ||||||
![]() | TRANSFERASE / HERBICIDE DETOXIFICATION | ||||||
Function / homology | ![]() response to herbicide / glutathione transferase / glutathione transferase activity / glutathione metabolic process / protein homodimerization activity / protein-containing complex Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() | ||||||
![]() | Neuefeind, T. / Huber, R. / Reinemer, P. / Knaeblein, J. | ||||||
![]() | ![]() Title: Cloning, sequencing, crystallization and X-ray structure of glutathione S-transferase-III from Zea mays var. mutin: a leading enzyme in detoxification of maize herbicides. Authors: Neuefeind, T. / Huber, R. / Reinemer, P. / Knablein, J. / Prade, L. / Mann, K. / Bieseler, B. #1: ![]() Title: Crystal Structure of Herbicide-Detoxifying Maize Glutathione S-Transferase-I in Complex with Lactoylglutathione: Evidence for an Induced-Fit Mechanism Authors: Neuefeind, T. / Huber, R. / Dasenbrock, H. / Prade, L. / Bieseler, B. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 70.4 KB | Display | ![]() |
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PDB format | ![]() | 53.2 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 364.1 KB | Display | ![]() |
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Full document | ![]() | 367.1 KB | Display | |
Data in XML | ![]() | 6.2 KB | Display | |
Data in CIF | ![]() | 9.6 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 23456.945 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Details: APO FORM / Source: (gene. exp.) ![]() ![]() ![]() ![]() | ||
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#2: Chemical | ChemComp-CD / #3: Water | ChemComp-HOH / | |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 3.42 Å3/Da / Density % sol: 64.05 % | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Crystal grow | pH: 7.5 / Details: pH 7.5 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Crystal grow | *PLUS Method: vapor diffusion, hanging drop / pH: 7 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 290 K |
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Diffraction source | Source: ![]() |
Detector | Type: MARRESEARCH / Detector: IMAGE PLATE / Date: Mar 1, 1995 |
Radiation | Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.5418 Å / Relative weight: 1 |
Reflection | Num. obs: 15788 / % possible obs: 99.5 % / Redundancy: 9.5 % / Rmerge(I) obs: 0.087 |
Reflection shell | Highest resolution: 1.97 Å |
Reflection | *PLUS Highest resolution: 2.2 Å |
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Processing
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Refinement | Resolution: 2.2→8 Å /
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Displacement parameters | Biso mean: 161 Å2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.2→8 Å
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Refine LS restraints |
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Software | *PLUS Name: ![]() | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement | *PLUS Rfactor Rfree: 0.224 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints | *PLUS
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LS refinement shell | *PLUS Highest resolution: 2 Å / Lowest resolution: 2.22 Å / Rfactor obs: 0.298 |