[English] 日本語
![](img/lk-miru.gif)
- PDB-1hxq: THE STRUCTURE OF NUCLEOTIDYLATED GALACTOSE-1-PHOSPHATE URIDYLYLTR... -
+
Open data
-
Basic information
Entry | Database: PDB / ID: 1hxq | ||||||
---|---|---|---|---|---|---|---|
Title | THE STRUCTURE OF NUCLEOTIDYLATED GALACTOSE-1-PHOSPHATE URIDYLYLTRANSFERASE FROM ESCHERICHIA COLI AT 1.86 ANGSTROMS RESOLUTION | ||||||
![]() | HEXOSE-1-PHOSPHATE URIDYLYLTRANSFERASE | ||||||
![]() | NUCLEOTIDYLTRANSFERASE / METALLOENZYME / NUCLEOTIDYLATED HISTIDINE / REACTION INTERMEDIATE | ||||||
Function / homology | ![]() UDP-glucose-hexose-1-phosphate uridylyltransferase / UDP-glucose:hexose-1-phosphate uridylyltransferase activity / galactokinase activity / galactose catabolic process via UDP-galactose / ferrous iron binding / zinc ion binding / cytoplasm / cytosol Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() | ||||||
![]() | Wedekind, J.E. / Frey, P.A. / Rayment, I. | ||||||
![]() | ![]() Title: The structure of nucleotidylated histidine-166 of galactose-1-phosphate uridylyltransferase provides insight into phosphoryl group transfer. Authors: Wedekind, J.E. / Frey, P.A. / Rayment, I. #1: ![]() Title: Three-Dimensional Structure of Galactose-1-Phosphate Uridylyltransferase from Escherichia Coli at 1.8 A Resolution Authors: Wedekind, J.E. / Frey, P.A. / Rayment, I. #2: ![]() Title: Galactose-1-Phosphate Uridylyltransferase from Escherichia Coli, a Zinc and Iron Metalloenzyme Authors: Ruzicka, F.J. / Wedekind, J.E. / Kim, J. / Rayment, I. / Frey, P.A. #3: ![]() Title: Crystallization and Preliminary Crystallographic Analysis of Galactose-1-Phosphate Uridylyltransferase from Escherichia Coli Authors: Wedekind, J.E. / Frey, P.A. / Rayment, I. #4: ![]() Title: The Nucleotide Sequence of the Gal T Gene of Escherichia Coli Authors: Cornwell, T.L. / Adhya, S.L. / Reznikoff, W.S. / Frey, P.A. | ||||||
History |
|
-
Structure visualization
Structure viewer | Molecule: ![]() ![]() |
---|
-
Downloads & links
-
Download
PDBx/mmCIF format | ![]() | 161.6 KB | Display | ![]() |
---|---|---|---|---|
PDB format | ![]() | 132.3 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 491.4 KB | Display | ![]() |
---|---|---|---|---|
Full document | ![]() | 505.2 KB | Display | |
Data in XML | ![]() | 16.7 KB | Display | |
Data in CIF | ![]() | 29.1 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Similar structure data |
---|
-
Links
-
Assembly
Deposited unit | ![]()
| ||||||||
---|---|---|---|---|---|---|---|---|---|
1 |
| ||||||||
Unit cell |
| ||||||||
Noncrystallographic symmetry (NCS) | NCS oper: (Code: given Matrix: (0.94373, -0.00281, 0.33071), Vector: Details | THE CRYSTALLOGRAPHICALLY INDEPENDENT UNIT IS ONE DIMER OF CHEMICALLY IDENTICAL SUBUNITS. | |
-
Components
#1: Protein | Mass: 39694.598 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() References: UniProt: P09148, UDP-glucose-hexose-1-phosphate uridylyltransferase #2: Chemical | #3: Chemical | #4: Chemical | #5: Water | ChemComp-HOH / | Compound details | GLN 168 MAKES CLOSE CONTACTS TO O2 OF THE U5P PHOSPHORYL GROUP. MUTATION OF THIS RESIDUE TO ARG HAS ...GLN 168 MAKES CLOSE CONTACTS TO O2 OF THE U5P PHOSPHORYL | Nonpolymer details | THIS ENTRY REPRESENTS THE STRUCTURE OF GALACTOSE-1-PHOSPHATE URIDYLYLTRANSFERASE IN WHICH NE2 OF ...THIS ENTRY REPRESENTS | |
---|
-Experimental details
-Experiment
Experiment | Method: ![]() |
---|
-
Sample preparation
Crystal | Density Matthews: 2.77 Å3/Da / Density % sol: 55.6 % | ||||||||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Crystal grow | pH: 7.1 Details: EACH CRYSTAL WAS REACTED WITH SUBSTRATE UDP-GLUCOSE IN A SYNTHETIC MOTHER LIQUOR DEVOID OF LITHIUM SULFATE AT PH 7.1. | ||||||||||||||||||||||||||||||
Crystal grow | *PLUS pH: 6 / Method: vapor diffusion | ||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
|
-Data collection
Diffraction | Mean temperature: 113 K |
---|---|
Diffraction source | Wavelength: 1.5418 |
Detector | Type: SIEMENS HI-STAR / Detector: AREA DETECTOR / Date: 1995 / Details: SUPPER DOUBLE-FOCUSING MIRRORS WITH NICKEL FOIL |
Radiation | Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.5418 Å / Relative weight: 1 |
Reflection | Resolution: 1.86→65 Å / Num. obs: 70581 / % possible obs: 97 % / Observed criterion σ(I): -1 / Redundancy: 2.5 % / Biso Wilson estimate: 19.1 Å2 / Rmerge(I) obs: 0.063 |
Reflection | *PLUS Num. all: 15045 / Num. measured all: 206202 / Rmerge(I) obs: 0.0625 |
Reflection shell | *PLUS Highest resolution: 1.86 Å / % possible obs: 90 % / Num. unique obs: 10989 / Num. measured obs: 19085 / Rmerge(I) obs: 0.165 |
-
Processing
Software |
| ||||||||||||||||||||||||||||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Refinement | Resolution: 1.86→65 Å / σ(F): 0 Details: REGIONS A 36 THROUGH A 44 AND B 31 THROUGH B 43 ARE DISORDERED. NO SIGNIFICANT ELECTRON DENSITY CAN BE SEEN FOR THESE REGIONS AT THE RESOLUTION OF THE REFINED MODEL. RESIDUES B 347 AND B 348 ...Details: REGIONS A 36 THROUGH A 44 AND B 31 THROUGH B 43 ARE DISORDERED. NO SIGNIFICANT ELECTRON DENSITY CAN BE SEEN FOR THESE REGIONS AT THE RESOLUTION OF THE REFINED MODEL. RESIDUES B 347 AND B 348 OF SUBUNIT II ARE DISORDERED AS WELL.
| ||||||||||||||||||||||||||||||||||||||||||||||||||
Solvent computation | Solvent model: TNT / Bsol: 526 Å2 / ksol: 0.7 e/Å3 | ||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.86→65 Å
| ||||||||||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints |
| ||||||||||||||||||||||||||||||||||||||||||||||||||
Software | *PLUS Name: TNT / Version: 5-E / Classification: refinement | ||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement | *PLUS Rfactor all: 0.196 | ||||||||||||||||||||||||||||||||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints | *PLUS
|