+Open data
-Basic information
Entry | Database: PDB / ID: 1hwu | ||||||
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Title | STRUCTURE OF PII PROTEIN FROM HERBASPIRILLUM SEROPEDICAE | ||||||
Components | PII PROTEIN | ||||||
Keywords | SIGNALING PROTEIN / Herbaspirillum seropedicae PII / Beta-alpha-beta motif / signal transduction protein | ||||||
Function / homology | Function and homology information | ||||||
Biological species | Herbaspirillum seropedicae (bacteria) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.1 Å | ||||||
Authors | Benelli, E.M. / Buck, M. / Polikarpov, I. / De Souza, E.M. / Cruz, L.M. / Pedrosa, F.O. | ||||||
Citation | Journal: Eur.J.Biochem. / Year: 2002 Title: Herbaspirillum seropedicae signal transduction protein PII is structurally similar to the enteric GlnK. Authors: Machado Benelli, E. / Buck, M. / Polikarpov, I. / Maltempi de Souza, E. / Cruz, L.M. / Pedrosa, F.O. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1hwu.cif.gz | 116.4 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1hwu.ent.gz | 92.9 KB | Display | PDB format |
PDBx/mmJSON format | 1hwu.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/hw/1hwu ftp://data.pdbj.org/pub/pdb/validation_reports/hw/1hwu | HTTPS FTP |
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-Related structure data
Related structure data | 2piiS S: Starting model for refinement |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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3 |
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Unit cell |
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-Components
#1: Protein | Mass: 12284.189 Da / Num. of mol.: 6 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Herbaspirillum seropedicae (bacteria) / Gene: GLNB / Plasmid: PET28B+ / Production host: Escherichia coli (E. coli) / Strain (production host): RB9065(LAMBDA)DE3 / References: UniProt: P94852 #2: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.21 Å3/Da / Density % sol: 44.33 % | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Crystal grow | Temperature: 291 K / Method: vapor diffusion, hanging drop / pH: 6.5 Details: Sodium Cacodylate, Magnesium Acetate, Methylpentadiol, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Crystal grow | *PLUS pH: 8 / Method: vapor diffusion, hanging drop | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 120 K |
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Diffraction source | Source: SYNCHROTRON / Site: LNLS / Beamline: D03B-MX1 / Wavelength: 1.38 Å |
Detector | Type: MARRESEARCH / Detector: IMAGE PLATE / Date: Jun 18, 1998 |
Radiation | Monochromator: Si 111 CHANNEL / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.38 Å / Relative weight: 1 |
Reflection | Resolution: 2.1→13 Å / Num. all: 146413 / Num. obs: 36523 / % possible obs: 94.4 % / Observed criterion σ(I): 3 / Redundancy: 4 % / Rmerge(I) obs: 0.057 |
Reflection shell | Resolution: 2.1→2.15 Å / Rmerge(I) obs: 0.322 / Num. unique all: 36523 / % possible all: 95.5 |
Reflection | *PLUS % possible obs: 94 % / Redundancy: 3 % |
Reflection shell | *PLUS Highest resolution: 2.1 Å / % possible obs: 95 % / Num. unique obs: 2415 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: PDB ENTRY 2PII Resolution: 2.1→13 Å / Cross valid method: THROUGHOUT
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Refinement step | Cycle: LAST / Resolution: 2.1→13 Å
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Refine LS restraints |
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Refinement | *PLUS Num. reflection obs: 36331 / Num. reflection Rfree: 1820 / % reflection Rfree: 5 % | ||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||
Displacement parameters | *PLUS |