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Open data
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Basic information
Entry | Database: PDB / ID: 2pii | ||||||
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Title | PII, GLNB PRODUCT | ||||||
![]() | PII | ||||||
![]() | SIGNAL TRANSDUCTION PROTEIN | ||||||
Function / homology | ![]() regulation of fatty acid biosynthetic process / regulation of nitrogen utilization / small molecule binding / enzyme regulator activity / enzyme activator activity / ATP binding / identical protein binding / cytosol Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() | ||||||
![]() | Carr, P.D. / Cheah, E. / Suffolk, P.M. / Ollis, D.L. | ||||||
![]() | ![]() Title: X-ray structure of the signal transduction protein from Escherichia coli at 1.9 A. Authors: Carr, P.D. / Cheah, E. / Suffolk, P.M. / Vasudevan, S.G. / Dixon, N.E. / Ollis, D.L. #1: ![]() Title: Escherichia Coli Pii Protein: Purification, Crystallization and Oligomeric Structure Authors: Vasudevan, S.G. / Gedye, C. / Dixon, N.E. / Cheah, E. / Carr, P.D. / Suffolk, P.M. / Jeffrey, P.D. / Ollis, D.L. #2: ![]() Title: Structure of the Escherichia Coli Signal Transducing Protein Pii Authors: Cheah, E. / Carr, P.D. / Suffolk, P.M. / Vasudevan, S.G. / Dixon, N.E. / Ollis, D.L. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 40.3 KB | Display | ![]() |
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PDB format | ![]() | 28.5 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 360.8 KB | Display | ![]() |
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Full document | ![]() | 362.9 KB | Display | |
Data in XML | ![]() | 3.8 KB | Display | |
Data in CIF | ![]() | 7 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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Unit cell |
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Components on special symmetry positions |
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Components
#1: Protein | Mass: 12443.442 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() |
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#2: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.48 Å3/Da / Density % sol: 53 % | ||||||||||||||||||||
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Crystal grow | *PLUS Temperature: 4 ℃ / pH: 7 / Method: vapor diffusion, hanging drop / Details: Vasudevan, S.G., (1994) FEBS Lett., 337, 255. | ||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Radiation | Scattering type: x-ray |
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Radiation wavelength | Relative weight: 1 |
Reflection | Num. obs: 11079 / Observed criterion σ(I): 1 |
Reflection | *PLUS Highest resolution: 1.9 Å / Lowest resolution: 10 Å / Num. measured all: 45387 / Rmerge(I) obs: 0.071 |
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Processing
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Refinement | Resolution: 1.9→10 Å / σ(F): 3 /
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Refinement step | Cycle: LAST / Resolution: 1.9→10 Å
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Refine LS restraints |
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Refinement | *PLUS Rfactor obs: 0.132 / Rfactor Rwork: 0.132 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | *PLUS |