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- PDB-1hv1: DISSECTING ELECTROSTATIC INTERACTIONS AND THE PH-DEPENDENT ACTIVI... -
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Open data
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Basic information
Entry | Database: PDB / ID: 1hv1 | ||||||
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Title | DISSECTING ELECTROSTATIC INTERACTIONS AND THE PH-DEPENDENT ACTIVITY OF A FAMILY 11 GLYCOSIDASE | ||||||
![]() | ENDO-1,4-BETA-XYLANASE | ||||||
![]() | HYDROLASE / beta sheet | ||||||
Function / homology | ![]() endo-1,4-beta-xylanase activity / endo-1,4-beta-xylanase / xylan catabolic process Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() | ||||||
![]() | Joshi, M.D. / Sidhu, G. / Nielsen, J.E. / Brayer, G.D. / Withers, S.G. / McIntosh, L.P. | ||||||
![]() | ![]() Title: Dissecting the electrostatic interactions and pH-dependent activity of a family 11 glycosidase. Authors: Joshi, M.D. / Sidhu, G. / Nielsen, J.E. / Brayer, G.D. / Withers, S.G. / McIntosh, L.P. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 51.4 KB | Display | ![]() |
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PDB format | ![]() | 36.2 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 407.3 KB | Display | ![]() |
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Full document | ![]() | 408.4 KB | Display | |
Data in XML | ![]() | 10.5 KB | Display | |
Data in CIF | ![]() | 15.1 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 1hv0C ![]() 1bcxS S: Starting model for refinement C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 20351.955 Da / Num. of mol.: 1 / Fragment: Q127A_BCX / Mutation: Q127A Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() |
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#2: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.23 Å3/Da / Density % sol: 44.77 % | ||||||||||||||||||||||||||||||
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Crystal grow | Temperature: 298 K / Method: vapor diffusion, hanging drop / pH: 7.5 Details: ammonimu sulphate, sodium chloride, TRIS, pH 7.5, VAPOR DIFFUSION, HANGING DROP at 298 K | ||||||||||||||||||||||||||||||
Crystal grow | *PLUS Details: Sidhu, G., (1999) Biochemistry, 38, 5346. | ||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 298 K |
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Diffraction source | Source: ![]() |
Detector | Type: RIGAKU RAXIS IIC / Detector: IMAGE PLATE / Date: Oct 1, 1999 |
Radiation | Monochromator: graphite / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.5418 Å / Relative weight: 1 |
Reflection | Resolution: 1.8→50 Å / Num. obs: 17548 / Observed criterion σ(F): 0 / Observed criterion σ(I): 0 / Redundancy: 9 % / Rmerge(I) obs: 0.054 / Net I/σ(I): 21.8 |
Reflection shell | Resolution: 1.8→1.88 Å / Rmerge(I) obs: 0.115 |
Reflection | *PLUS Lowest resolution: 9999 Å / Num. measured all: 157188 |
Reflection shell | *PLUS Mean I/σ(I) obs: 6.6 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: PDB ENTRY 1BCX Resolution: 1.8→10 Å / σ(F): 0
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Refinement step | Cycle: LAST / Resolution: 1.8→10 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 1.8→10 Å
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Software | *PLUS Name: ![]() | ||||||||||||||||||
Refinement | *PLUS Highest resolution: 1.8 Å / Lowest resolution: 10 Å / σ(F): 0 | ||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||
Displacement parameters | *PLUS | ||||||||||||||||||
LS refinement shell | *PLUS Highest resolution: 1.8 Å / Lowest resolution: 10 Å / Rfactor Rwork: 0.164 |