+Open data
-Basic information
Entry | Database: PDB / ID: 1hn9 | |||||||||
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Title | CRYSTAL STRUCTURE OF BETA-KETOACYL-ACP SYNTHASE III | |||||||||
Components | BETA-KETOACYL-ACYL CARRIER PROTEIN SYNTHASE III | |||||||||
Keywords | TRANSFERASE / FabH | |||||||||
Function / homology | Function and homology information beta-ketoacyl-[acyl-carrier-protein] synthase III / beta-ketoacyl-acyl-carrier-protein synthase III activity / 3-oxoacyl-[acyl-carrier-protein] synthase activity / fatty acid metabolic process / fatty acid biosynthetic process / cytosol Similarity search - Function | |||||||||
Biological species | Escherichia coli (E. coli) | |||||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2 Å | |||||||||
Authors | Qiu, X. / Janson, C.A. / Konstantinidis, A.K. / Nwagwu, S. / Silverman, C. / Smith, W.W. / Khandekar, S.K. / Lonsdale, J. / Abdel-Meguid, S.S. | |||||||||
Citation | Journal: J.Biol.Chem. / Year: 1999 Title: Crystal structure of beta-ketoacyl-acyl carrier protein synthase III. A key condensing enzyme in bacterial fatty acid biosynthesis. Authors: Qiu, X. / Janson, C.A. / Konstantinidis, A.K. / Nwagwu, S. / Silverman, C. / Smith, W.W. / Khandekar, S. / Lonsdale, J. / Abdel-Meguid, S.S. | |||||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1hn9.cif.gz | 137.1 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1hn9.ent.gz | 106.4 KB | Display | PDB format |
PDBx/mmJSON format | 1hn9.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 1hn9_validation.pdf.gz | 383 KB | Display | wwPDB validaton report |
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Full document | 1hn9_full_validation.pdf.gz | 390.3 KB | Display | |
Data in XML | 1hn9_validation.xml.gz | 13.4 KB | Display | |
Data in CIF | 1hn9_validation.cif.gz | 22.9 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/hn/1hn9 ftp://data.pdbj.org/pub/pdb/validation_reports/hn/1hn9 | HTTPS FTP |
-Related structure data
Related structure data | |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 33547.027 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Escherichia coli (E. coli) / Production host: Escherichia coli (E. coli) References: UniProt: P0A6R0, beta-ketoacyl-[acyl-carrier-protein] synthase I #2: Chemical | #3: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.55 Å3/Da / Density % sol: 51.67 % |
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Crystal grow | Temperature: 298 K / Method: vapor diffusion, sitting drop / pH: 7 Details: PEK4000, pH 7, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
Crystal grow | *PLUS Method: unknown |
Components of the solutions | *PLUS Conc.: 20 % / Common name: PEG8000 |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: SYNCHROTRON / Site: APS / Beamline: 17-ID / Wavelength: 1 Å |
Detector | Type: MARRESEARCH / Detector: CCD / Date: Apr 1, 1999 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1 Å / Relative weight: 1 |
Reflection | Resolution: 2→20 Å / Num. obs: 292572 / % possible obs: 97 % / Observed criterion σ(F): 2 / Observed criterion σ(I): 2 / Redundancy: 6 % / Rmerge(I) obs: 0.077 |
Reflection shell | Resolution: 2→2.1 Å / Redundancy: 7 % / Rmerge(I) obs: 0.042 / Mean I/σ(I) obs: 3.5 / % possible all: 97 |
Reflection shell | *PLUS % possible obs: 97 % |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: FabH-acetyl-CoA complex Resolution: 2→20 Å / σ(F): 0 / σ(I): 0
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Refinement step | Cycle: LAST / Resolution: 2→20 Å
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Refine LS restraints |
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