Mass: 18.015 Da / Num. of mol.: 199 / Source method: isolated from a natural source / Formula: H2O
Compound details
HSP82 IS AN ESSENTIAL PROTEIN THAT IS REQUIRED BY CELLS FOR GROWTH AT HIGHER TEMPERATURES.THIS ...HSP82 IS AN ESSENTIAL PROTEIN THAT IS REQUIRED BY CELLS FOR GROWTH AT HIGHER TEMPERATURES.THIS PROTEIN IS A MOLECULAR CHAPERONE WITH ATPASE ACTIVITY. GENERALLY EXPRESSED AT LOW LEVELS BUT IS STRONGLY INDUCED AT HIGH TEMPERATURES.
Sequence details
MIDDLE DOMAIN ONLY,RESIDUES 273-560
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Experimental details
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Experiment
Experiment
Method: X-RAY DIFFRACTION / Number of used crystals: 1
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Sample preparation
Crystal
Density Matthews: 3.25 Å3/Da / Density % sol: 62 %
Crystal grow
Method: microbatch / pH: 7.5 Details: CRYSTALS GROWN USING MICROBATCH METHOD BY MIXING 1UL OF 24MG/ML,PROTEIN IN BUFFER (20MM TRISHCL),PH 7.5, 1MM EDTA, 0.5MM DTT) WITH 1UL OF 11MM CDSO4, 20MM MGCL2, 80MM TRIS HCL, PH 7.5 AND 5% GLYCEROL.
% possible obs: 99.9 % / Rmerge(I) obs: 0.379 / Mean I/σ(I) obs: 1.9
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Processing
Software
Name
Version
Classification
CNS
1.1
refinement
MOSFLM
datareduction
SCALA
datascaling
CNS
phasing
Refinement
Method to determine structure: MAD / Resolution: 2.5→29.68 Å / Rfactor Rfree error: 0.005 / Data cutoff high absF: 1667469.42 / Isotropic thermal model: RESTRAINED / Cross valid method: THROUGHOUT / σ(F): 0 Details: LOOP A378-A384 NOT VISIBLE IN STRUCTURE. LOOP B332-B340 NOT VISIBLE IN STRUCTURE. C-TERMINUS A528-A560 NOT VISIBLE IN STRUCTURE AND C-TERMINUS B529-B560 NOT VISIBLE IN STRUCTURE.
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