+Open data
-Basic information
Entry | Database: PDB / ID: 1hh0 | ||||||
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Title | Filamentous Bacteriophage PH75 | ||||||
Components | PH75 INOVIRUS MAJOR COAT PROTEIN | ||||||
Keywords | VIRUS / VIRUS COAT PROTEIN / HELICAL VIRUS COAT PROTEIN / SSDNA VIRUSES / INOVIRUS / FILAMENTOUS BACTERIOPHAGE / THERMOPHILES / MEMBRANE PROTEINS / HELICAL VIRUS | ||||||
Function / homology | Single alpha-helices involved in coiled-coils or other helix-helix interfaces - #80 / Bacteriophage M13, G8P, capsid domain superfamily / helical viral capsid / host cell membrane / Single alpha-helices involved in coiled-coils or other helix-helix interfaces / Up-down Bundle / Mainly Alpha / membrane / Capsid protein G8P Function and homology information | ||||||
Biological species | BACTERIOPHAGE PH75 (virus) | ||||||
Method | FIBER DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.4 Å | ||||||
Authors | Pederson, D.M. / Welsh, L.C. / Marvin, D.A. / Sampson, M. / Perham, R.N. / Yu, M. / Slater, M.R. | ||||||
Citation | Journal: J.Mol.Biol. / Year: 2001 Title: The Protein Capsid of Filamentous Bacteriophage Ph75 from Thermus Thermophilus Authors: Pederson, D.M. / Welsh, L.C. / Marvin, D.A. / Sampson, M. / Perham, R.N. / Yu, M. / Slater, M.R. #1: Journal: Acta Crystallogr.,Sect.D / Year: 2000 Title: The Molecular Structure and Structural Transition of the Alpha-Helical Capsid in Filamentous Bacteriophage Pf1 Authors: Welsh, L.C. / Symmons, M.F. / Marvin, D.A. #2: Journal: J.Mol.Biol. / Year: 1998 Title: Structure of the Capsid of Pf3 Filamentous Phage Determined from X-Ray Fibre Diffraction Data at 3.1 A Resolution Authors: Welsh, L.C. / Symmons, M.F. / Sturtevant, J.M. / Marvin, D.A. / Perham, R.N. #3: Journal: Acta Crystallogr.,Sect.D / Year: 1995 Title: Pf1 Filamentous Bacteriophage: Refinement of a Molecular Model by Simulated Annealing Using 3.3 Angstroms Resolution X-Ray Fibre Diffraction Data Authors: Gonzalez, A. / Nave, C. / Marvin, D.A. #4: Journal: Int.J.Biol.Macromol. / Year: 1990 Title: Model-Building Studies of Inovirus: Genetic Variations on a Geometric Theme Authors: Marvin, D.A. | ||||||
History |
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Remark 650 | HELIX DETERMINATION METHOD: AUTHOR PROVIDED. |
-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1hh0.cif.gz | 26.5 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1hh0.ent.gz | 17.5 KB | Display | PDB format |
PDBx/mmJSON format | 1hh0.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/hh/1hh0 ftp://data.pdbj.org/pub/pdb/validation_reports/hh/1hh0 | HTTPS FTP |
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-Related structure data
Related structure data | 1hgvC 1hgzC 1ifnS S: Starting model for refinement C: citing same article (ref.) |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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2 |
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3 |
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Unit cell |
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Symmetry | Helical symmetry: (Circular symmetry: 1 / Dyad axis: no / N subunits divisor: 1 / Num. of operations: 35 / Rise per n subunits: 2.9 Å / Rotation per n subunits: 66.667 °) |
-Components
#1: Protein/peptide | Mass: 4816.578 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Details: MAJOR COAT PROTEIN ASSEMBLY / Source: (gene. exp.) BACTERIOPHAGE PH75 (virus) / Description: GROWN IN THERMUS THERMOPHILUS / Production host: THERMUS THERMOPHILUS HB8 (bacteria) / References: UniProt: P82889 |
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-Experimental details
-Experiment
Experiment | Method: FIBER DIFFRACTION |
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-Sample preparation
Crystal | Description: THE DATA IS DERIVED FROM CONTINUOUS TRANSFORM DATA AND THEREFORE THE NUMBER OF UNIQUE REFLECTIONS IS A MEANINGLESS NUMBER. THE STRUCTURE WAS REFINED AGAI THE SAME STRUCTURE FACTORS AS ...Description: THE DATA IS DERIVED FROM CONTINUOUS TRANSFORM DATA AND THEREFORE THE NUMBER OF UNIQUE REFLECTIONS IS A MEANINGLESS NUMBER. THE STRUCTURE WAS REFINED AGAI THE SAME STRUCTURE FACTORS AS PDB ENTRY 1HGV, USING DIFFERENT REFINEMENT PROTOCOL. THEREFORE, THE STRUCT FACTORS FOR 1HHO, CAN BE TAKEN FROM R1HGVSF |
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Crystal grow | pH: 7.5 / Details: pH 7.50 |
Crystal grow | *PLUS Method: unknown |
-Data collection
Diffraction | Mean temperature: 300 K |
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Diffraction source | Source: SYNCHROTRON / Site: SRS / Beamline: PX7.2 / Wavelength: 1.488 |
Detector | Type: MARRESEARCH / Detector: IMAGE PLATE / Date: Feb 15, 1999 / Details: MIRRORS |
Radiation | Monochromator: GE CRYSTAL / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.488 Å / Relative weight: 1 |
Reflection | Resolution: 2.4→50 Å / % possible obs: 100 % |
Reflection | *PLUS % possible obs: 100 % |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: 1IFN Resolution: 2.4→50 Å / σ(F): 0 Details: THE MODEL WAS DERIVED FROM PDB ENTRY 1IFN, REFERENCE 1, BY MOLECULAR REPLACEMENT
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Refinement step | Cycle: LAST / Resolution: 2.4→50 Å
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Refine LS restraints |
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Software | *PLUS Name: FXPLOR / Classification: refinement | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement | *PLUS Rfactor obs: 0.22 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints | *PLUS
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