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- PDB-1ha8: Pheromone Er-23 from Euplotes raikovi -

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Basic information

Entry
Database: PDB / ID: 1ha8
TitlePheromone Er-23 from Euplotes raikovi
ComponentsPHEROMONE
KeywordsPHEROMONE
Function / homologyPheromone Er-23, protozoan / Arc Repressor Mutant, subunit A - #190 / Pheromone Er-2/Er-23, protozoan / mating pheromone activity / Arc Repressor Mutant, subunit A / extracellular region / Orthogonal Bundle / Mainly Alpha / Mating pheromone Er-23
Function and homology information
Biological speciesEUPLOTES RAIKOVI (eukaryote)
MethodSOLUTION NMR / torsion angle dynamics
AuthorsZahn, R. / Damberger, F. / Luporini, P. / Wuthrich, K.
CitationJournal: J.Mol.Biol. / Year: 2001
Title: NMR Structure of the Euplotes Raikovi Pheromone Er- 23 and Identification of its Five Disulfide Bonds
Authors: Zahn, R. / Damberger, F. / Ortenzi, C. / Luporini, P. / Wuthrich, K.
History
DepositionMar 30, 2001Deposition site: PDBE / Processing site: PDBE
Revision 1.0Nov 27, 2001Provider: repository / Type: Initial release
Revision 1.1May 7, 2011Group: Version format compliance
Revision 1.2Jul 13, 2011Group: Version format compliance
Revision 1.3Jun 14, 2017Group: Structure summary / Category: pdbx_nmr_representative / Item: _pdbx_nmr_representative.conformer_id
Revision 1.4Jan 15, 2020Group: Other / Category: pdbx_database_status
Item: _pdbx_database_status.status_code_cs / _pdbx_database_status.status_code_mr
Revision 1.5Jun 14, 2023Group: Database references / Other / Category: database_2 / pdbx_database_status
Item: _database_2.pdbx_DOI / _database_2.pdbx_database_accession / _pdbx_database_status.status_code_nmr_data
Remark 650 HELIX DETERMINATION METHOD: AUTHOR PROVIDED.

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
A: PHEROMONE


Theoretical massNumber of molelcules
Total (without water)5,1051
Polymers5,1051
Non-polymers00
Water00
1


  • Idetical with deposited unit
  • defined by author
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
NMR ensembles
DataCriteria
Number of conformers (submitted / calculated)20 / 100LEAST RESTRAINT VIOLATION
RepresentativeModel #9

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Components

#1: Protein PHEROMONE / ER-23


Mass: 5104.520 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) EUPLOTES RAIKOVI (eukaryote) / Cellular location: EXTRACELLULAR / References: UniProt: P58547*PLUS

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Experimental details

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Experiment

ExperimentMethod: SOLUTION NMR
NMR experiment
Conditions-IDExperiment-IDSolution-IDType
111NOESY
121COSY

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Sample preparation

Sample conditionspH: 4.6 / Pressure: 1 atm / Temperature: 290 K
Crystal grow
*PLUS
Method: other / Details: NMR

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NMR measurement

NMR spectrometerType: Varian UNITYPLUS / Manufacturer: Varian / Model: UNITYPLUS / Field strength: 750 MHz

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Processing

NMR software
NameDeveloperClassification
DYANAGUNTERT,WUTHRICHrefinement
DYANAstructure solution
RefinementMethod: torsion angle dynamics / Software ordinal: 1
NMR ensembleConformer selection criteria: LEAST RESTRAINT VIOLATION / Conformers calculated total number: 100 / Conformers submitted total number: 20

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