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- PDB-6px8: Dg3b in Weaponisation 'on the fly': Convergent recruitment of kno... -
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Open data
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Basic information
Entry | Database: PDB / ID: 6px8 | ||||||
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Title | Dg3b in Weaponisation 'on the fly': Convergent recruitment of knottin and defensin scaffolds as neurotoxins in the venom of assassin fly Dolopus genitalis (Diptera: Asilidae) | ||||||
![]() | Venom polypeptide | ||||||
![]() | TOXIN / disulfide-rich / assassin-fly / defensin / ultra-stable peptide | ||||||
Function / homology | Arthropod defensin / Invertebrate defensins family profile. / Defensin, invertebrate/fungal / Knottin, scorpion toxin-like superfamily / defense response to bacterium / innate immune response / extracellular region / U-Asilidin(12)-Dg3b![]() | ||||||
Biological species | ![]() | ||||||
Method | SOLUTION NMR / torsion angle dynamics | ||||||
![]() | Agwa, A.J. / Schroeder, C. | ||||||
![]() | ![]() Title: Weaponisation 'on the fly': Convergent recruitment of knottin and defensin peptide scaffolds into the venom of predatory assassin flies. Authors: Jin, J. / Agwa, A.J. / Szanto, T.G. / Csoti, A. / Panyi, G. / Schroeder, C.I. / Walker, A.A. / King, G.F. | ||||||
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 220.2 KB | Display | ![]() |
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PDB format | ![]() | 191.2 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
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-Validation report
Summary document | ![]() | 395.9 KB | Display | ![]() |
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Full document | ![]() | 482.3 KB | Display | |
Data in XML | ![]() | 13.1 KB | Display | |
Data in CIF | ![]() | 22.2 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 6px7C C: citing same article ( |
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Similar structure data | |
Other databases |
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Links
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Assembly
Deposited unit | ![]()
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NMR ensembles |
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Components
#1: Protein/peptide | Mass: 4147.926 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||||||||||||||||||||||
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NMR experiment |
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Sample preparation
Details | Type: solution / Contents: 1 mg/mL 0 Dg3b, 90% H2O/10% D2O / Label: Dg3b / Solvent system: 90% H2O/10% D2O |
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Sample | Conc.: 1 mg/mL / Component: Dg3b / Isotopic labeling: 0 |
Sample conditions | Ionic strength: 0 M / Label: Dg3b / pH: 4 / Pressure: 1 Pa / Temperature: 298 K |
-NMR measurement
NMR spectrometer | Type: Bruker AVANCE / Manufacturer: Bruker / Model: AVANCE / Field strength: 600 MHz |
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Processing
NMR software |
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Refinement | Method: torsion angle dynamics / Software ordinal: 4 | |||||||||||||||
NMR representative | Selection criteria: fewest violations | |||||||||||||||
NMR ensemble | Conformer selection criteria: structures with the least restraint violations Conformers calculated total number: 50 / Conformers submitted total number: 20 |