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- PDB-1h9u: The structure of the human retinoid-X-receptor beta ligand bindin... -
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Open data
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Basic information
Entry | Database: PDB / ID: 1h9u | ||||||
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Title | The structure of the human retinoid-X-receptor beta ligand binding domain in complex with the specific synthetic agonist LG100268 | ||||||
![]() | RETINOID X RECEPTOR, BETA | ||||||
![]() | NUCLEAR RECEPTOR / RXR / TRANSCRIPTION FACTOR | ||||||
Function / homology | ![]() retinoic acid-responsive element binding / NR1H2 & NR1H3 regulate gene expression linked to gluconeogenesis / NR1H2 & NR1H3 regulate gene expression linked to triglyceride lipolysis in adipose / NR1H2 & NR1H3 regulate gene expression to limit cholesterol uptake / NR1H2 & NR1H3 regulate gene expression linked to lipogenesis / positive regulation of vitamin D receptor signaling pathway / Signaling by Retinoic Acid / nuclear steroid receptor activity / NR1H2 & NR1H3 regulate gene expression to control bile acid homeostasis / retinoic acid receptor signaling pathway ...retinoic acid-responsive element binding / NR1H2 & NR1H3 regulate gene expression linked to gluconeogenesis / NR1H2 & NR1H3 regulate gene expression linked to triglyceride lipolysis in adipose / NR1H2 & NR1H3 regulate gene expression to limit cholesterol uptake / NR1H2 & NR1H3 regulate gene expression linked to lipogenesis / positive regulation of vitamin D receptor signaling pathway / Signaling by Retinoic Acid / nuclear steroid receptor activity / NR1H2 & NR1H3 regulate gene expression to control bile acid homeostasis / retinoic acid receptor signaling pathway / positive regulation of bone mineralization / response to retinoic acid / NR1H3 & NR1H2 regulate gene expression linked to cholesterol transport and efflux / hormone-mediated signaling pathway / RNA polymerase II transcription regulatory region sequence-specific DNA binding / mRNA transcription by RNA polymerase II / PPARA activates gene expression / Nuclear Receptor transcription pathway / RNA polymerase II transcription regulator complex / nuclear receptor activity / sequence-specific double-stranded DNA binding / DNA-binding transcription activator activity, RNA polymerase II-specific / cell differentiation / DNA-binding transcription factor activity, RNA polymerase II-specific / chromatin / nucleolus / positive regulation of DNA-templated transcription / positive regulation of transcription by RNA polymerase II / zinc ion binding / nucleoplasm / nucleus / cytosol Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Schwabe, J.W.R. / Love, J.D. / Gooch, J.T. | ||||||
![]() | ![]() Title: The Structural Basis for the Specificity of Retinoid-X Receptor-Selective Agonists: New Insights Into the Role of Helix H12 Authors: Love, J.D. / Gooch, J.T. / Benko, S. / Li, C. / Nagy, L. / Chatterjee, V.K.K. / Evans, R.M. / Schwabe, J.W.R. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 167.9 KB | Display | ![]() |
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PDB format | ![]() | 132.7 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Related structure data | ![]() 1lbd S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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Unit cell |
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Details | CRYSTAL. THE RETINOID X RECEPTOR IS ACTIVE AS DIMERIC |
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Components
#1: Protein | Mass: 24842.645 Da / Num. of mol.: 4 / Fragment: LIGAND BINDING DOMAIN RESIDUES 299-522 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() #2: Chemical | ChemComp-LG2 / #3: Chemical | ChemComp-NI / #4: Chemical | #5: Water | ChemComp-HOH / | Compound details | BINDS TO 9-CIS RETINOIC ACID (9C-RA). INVOLVED IN RETINOIC ACID RESPONSE PATHWAY. THE C-TERMINAL ...BINDS TO 9-CIS RETINOIC ACID (9C-RA). INVOLVED IN RETINOIC ACID RESPONSE PATHWAY. THE C-TERMINAL DOMAIN IS INVOLVED IN STEROID-BINDING. | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.82 Å3/Da / Density % sol: 56.45 % | |||||||||||||||||||||||||||||||||||||||||||||||||
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Crystal grow | pH: 7.6 / Details: pH 7.60 | |||||||||||||||||||||||||||||||||||||||||||||||||
Crystal grow | *PLUS Temperature: 20 ℃ / Method: vapor diffusion | |||||||||||||||||||||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: QUANTUM / Detector: CCD |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.933 Å / Relative weight: 1 |
Reflection | Resolution: 2.7→38 Å / Num. obs: 29713 / % possible obs: 98 % / Observed criterion σ(I): 2 / Redundancy: 2.9 % / Biso Wilson estimate: 55.3 Å2 / Rmerge(I) obs: 0.098 / Net I/σ(I): 5.1 |
Reflection | *PLUS Lowest resolution: 38 Å / % possible obs: 97.8 % / Num. measured all: 87647 |
Reflection shell | *PLUS Highest resolution: 2.7 Å / Lowest resolution: 2.87 Å / Rmerge(I) obs: 0.264 / Mean I/σ(I) obs: 2.1 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: PDB ENTRY 1LBD ![]() 1lbd Resolution: 2.7→38.14 Å / Rfactor Rfree error: 0.006 / Data cutoff high absF: 2064073.76 / Isotropic thermal model: RESTRAINED / Cross valid method: THROUGHOUT / σ(F): 2
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Solvent computation | Solvent model: FLAT MODEL / Bsol: 52.2394 Å2 / ksol: 0.35528 e/Å3 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 50.5 Å2
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Refine analyze |
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Refinement step | Cycle: LAST / Resolution: 2.7→38.14 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 2.7→2.87 Å / Rfactor Rfree error: 0.018 / Total num. of bins used: 6
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Xplor file |
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Refinement | *PLUS Rfactor obs: 0.277 / Rfactor Rfree: 0.301 / Rfactor Rwork: 0.277 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints | *PLUS
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