Journal: J.Mol.Biol. / Year: 2008 Title: A New Member of the Alkaline Phosphatase Superfamily with a Formylglycine Nucleophile: Structural and Kinetic Characterisation of a Phosphonate Monoester Hydrolase/Phosphodiesterase from Rhizobium Leguminosarum. Authors: Jonas, S. / Van Loo, B. / Hyvonen, M. / Hollfelder, F.
Mass: 18.015 Da / Num. of mol.: 646 / Source method: isolated from a natural source / Formula: H2O
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Details
Nonpolymer details
MANGANESE (II) ION (MN): TYPE OF METAL ION AND OCCUPANCY WERE ASSIGNED ON THE BASIS OF MICROPIXE ...MANGANESE (II) ION (MN): TYPE OF METAL ION AND OCCUPANCY WERE ASSIGNED ON THE BASIS OF MICROPIXE DATA AND VALENCE BOND CALCULATIONS CALCIUM ION (CA): TYPE OF METAL ION AND OCCUPANCY WERE ASSIGNED ON THE BASIS OF MICROPIXE DATA AND VALENCE BOND CALCULATIONS
Sequence details
RESIDUES 1-29 IN THE SEQUENCE ORIGINATE FROM A STREP-TAG AND A LINKER, THE ENZYME SEQUENCE STARTS ...RESIDUES 1-29 IN THE SEQUENCE ORIGINATE FROM A STREP-TAG AND A LINKER, THE ENZYME SEQUENCE STARTS WITH RESIDUE MET30 RESIDUE 57 IS A CASE OF MICROHETEROGENEITY WHERE MORE THAN ONE IDENTITY IS OBSERVED FOR A PARTICULAR RESIDUE NUMBER. AT POSITION 57 OF THE PROTEIN CHAIN A, BOTH DDZ AND CYS ARE PRESENT AT 0.60 AND 0.40 OCCUPANCIES RESPECTIVELY.
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Experimental details
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Experiment
Experiment
Method: X-RAY DIFFRACTION / Number of used crystals: 1
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Sample preparation
Crystal
Density Matthews: 2.11 Å3/Da / Density % sol: 41.7 % / Description: NONE