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- PDB-1h1l: NITROGENASE MO-FE PROTEIN FROM KLEBSIELLA PNEUMONIAE, NIFV MUTANT -
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Open data
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Basic information
Entry | Database: PDB / ID: 1h1l | ||||||
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Title | NITROGENASE MO-FE PROTEIN FROM KLEBSIELLA PNEUMONIAE, NIFV MUTANT | ||||||
![]() | (NITROGENASE MOLYBDENUM IRON PROTEIN ...) x 2 | ||||||
![]() | OXIDOREDUCTASE / BIOLOGICAL NITROGEN FIXATION / NITROGEN METABOLISM / MOLYBDOENZYMES / ELECTRON TRANSFER | ||||||
Function / homology | ![]() molybdenum-iron nitrogenase complex / nitrogenase / : / nitrogenase activity / nitrogen fixation / iron-sulfur cluster binding / ATP binding / metal ion binding Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Mayer, S.M. / Gormal, C.A. / Smith, B.E. / Lawson, D.M. | ||||||
![]() | ![]() Title: Crystallographic Analysis of the Mofe Protein of Nitrogenase from a Nifv Mutant of Klebsiella Pneumoniae Identifies Citrate as a Ligand to the Molybdenum of Iron Molybdenum Cofactor (Femoco). Authors: Mayer, S.M. / Gormal, C.A. / Smith, B.E. / Lawson, D.M. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 430.4 KB | Display | ![]() |
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PDB format | ![]() | 344.5 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 529.1 KB | Display | ![]() |
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Full document | ![]() | 567.4 KB | Display | |
Data in XML | ![]() | 87.6 KB | Display | |
Data in CIF | ![]() | 129.5 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 1qguS S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Noncrystallographic symmetry (NCS) | NCS oper:
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Components
-NITROGENASE MOLYBDENUM IRON PROTEIN ... , 2 types, 4 molecules ACBD
#1: Protein | Mass: 53919.387 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Details: CHAINS A AND C ARE ALPHA-SUBUNITS / Source: (natural) ![]() #2: Protein | Mass: 58338.180 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Details: CHAINS B AND D ARE BETA-SUBUNITS / Source: (natural) ![]() |
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-Non-polymers , 6 types, 1549 molecules ![](data/chem/img/CIT.gif)
![](data/chem/img/CFM.gif)
![](data/chem/img/MG.gif)
![](data/chem/img/CLF.gif)
![](data/chem/img/CL.gif)
![](data/chem/img/HOH.gif)
![](data/chem/img/CFM.gif)
![](data/chem/img/MG.gif)
![](data/chem/img/CLF.gif)
![](data/chem/img/CL.gif)
![](data/chem/img/HOH.gif)
#3: Chemical | #4: Chemical | #5: Chemical | ChemComp-MG / #6: Chemical | #7: Chemical | #8: Water | ChemComp-HOH / | |
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-Details
Compound details | NITROGEN FIXATION, CATALYZED BY THE NITROGENASE COMPLEX, THE IRON PROTEIN AND THE MOLYBDENUM-IRON ...NITROGEN FIXATION, CATALYZED BY THE NITROGENAS |
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Sequence details | N-TERMINAL SEQUENCING INDICATES THAT THE FIRST TWO AMINO ACIDS OF THE ALPHA SUBUNITS (CHAINS A AND ...N-TERMINAL SEQUENCING |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.31 Å3/Da / Density % sol: 46 % Description: PRIOR TO DATA COLLECTION THE CRYSTAL WAS SOAKED FOR 5 MINS IN CRYOPROTECTANT (MOTHER LIQUOR WITH 25% ETHYLENE GLYCOL CONTAINING 20 MM SODIUM DITHIONITE (A REDUCTANT). | ||||||||||||||||||||||||||||
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Crystal grow | Method: liquid diffusion / pH: 7.4 Details: CRYSTALLIZED ANAEROBICALLY USING LIQUID-LIQUID DIFFUSION TECHNIQUE. FINAL CONCENTRATIONS AFTER EQUILIBRATION WERE: 7% (W/V) PEG6000, 0.4 - 0.6 M MGCL2, 50 MM TRIS-HCL PH 7.4, 1.3 MG/ML PROTEIN. | ||||||||||||||||||||||||||||
Crystal grow | *PLUS Method: liquid-liquid diffusion | ||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: ADSC CCD / Detector: CCD / Date: Feb 8, 1999 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.936 Å / Relative weight: 1 |
Reflection | Resolution: 1.9→40 Å / Num. obs: 154203 / % possible obs: 94.2 % / Observed criterion σ(I): -3 / Redundancy: 3.9 % / Biso Wilson estimate: 20.3 Å2 / Rmerge(I) obs: 0.094 / Net I/σ(I): 15.5 |
Reflection shell | Resolution: 1.9→1.93 Å / Rmerge(I) obs: 0.214 / Mean I/σ(I) obs: 3.6 / % possible all: 85.2 |
Reflection | *PLUS Highest resolution: 1.9 Å / Lowest resolution: 40 Å |
Reflection shell | *PLUS % possible obs: 85.2 % / Mean I/σ(I) obs: 3.6 |
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Processing
Software |
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Refinement | Method to determine structure: ![]() Starting model: PDB ENTRY 1QGU Resolution: 1.9→40 Å / Cross valid method: THROUGHOUT / σ(F): 0 Details: TOWARDS THE END OF REFINEMENT ALL RESTRAIN WITHIN THE HETEROGENS CIT, CFM, AND CLF WERE EXPLICITL TURNED OFF. IN ADDITION, THE BONDS BETWEEN THESE HETEROGENS AND THE PROTEIN NOT RESTRAINED. ...Details: TOWARDS THE END OF REFINEMENT ALL RESTRAIN WITHIN THE HETEROGENS CIT, CFM, AND CLF WERE EXPLICITL TURNED OFF. IN ADDITION, THE BONDS BETWEEN THESE HETEROGENS AND THE PROTEIN NOT RESTRAINED. BOTH CITRATE MOLECULES WERE REFINED WITH AN OCCUPANCY OF 0.5. A NETWORK OF WATER MOLECULES WAS THOU TO BE PRESENT IN ITS ABSENCE. THREE WATERS WERE MODELLED IN PLA EACH CITRATE AND GIVEN OCCUPANCIES OF 0.5.
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Displacement parameters | Biso mean: 24 Å2 | ||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.9→40 Å
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Refinement | *PLUS Highest resolution: 1.9 Å | ||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||
Displacement parameters | *PLUS | ||||||||||||||||||||
Refine LS restraints | *PLUS
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