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Yorodumi- PDB-1h0t: An affibody in complex with a target protein: structure and coupl... -
+Open data
-Basic information
Entry | Database: PDB / ID: 1h0t | ||||||
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Title | An affibody in complex with a target protein: structure and coupled folding | ||||||
Components |
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Keywords | IMMUNE SYSTEM / PROTEIN-PROTEIN INTERACTIONS / PROTEIN ENGINEERING / MOLECULAR RECOGNITION / NMR SPECTROSCOPY / MOLTEN GLOBULE / INDUCED FIT / COUPLED PROTEIN FOLDING / AFFIBODY / IGG BINDING PROTEIN A | ||||||
Function / homology | Function and homology information | ||||||
Biological species | STAPHYLOCOCCUS AUREUS (bacteria) SYNTHETIC CONSTRUCT (others) | ||||||
Method | SOLUTION NMR / SIMULATED ANEALING | ||||||
Authors | Wahlberg, E. / Lendel, C. / Helgstrand, M. / Allard, P. / Dincbas-Renqvist, V. / Hedqvist, A. / Berglund, H. / Nygren, P.-A. / Hard, T. | ||||||
Citation | Journal: Proc.Natl.Acad.Sci.USA / Year: 2003 Title: An Affibody in Complex with a Target Protein: Structure and Coupled Folding Authors: Wahlberg, E. / Lendel, C. / Helgstrand, M. / Allard, P. / Dincbas-Renqvist, V. / Hedqvist, A. / Berglund, H. / Nygren, P.-A. / Hard, T. | ||||||
History |
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Remark 650 | HELIX DETERMINATION METHOD: AUTHOR PROVIDED. |
-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1h0t.cif.gz | 1.4 MB | Display | PDBx/mmCIF format |
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PDB format | pdb1h0t.ent.gz | 1.2 MB | Display | PDB format |
PDBx/mmJSON format | 1h0t.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 1h0t_validation.pdf.gz | 367 KB | Display | wwPDB validaton report |
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Full document | 1h0t_full_validation.pdf.gz | 943.6 KB | Display | |
Data in XML | 1h0t_validation.xml.gz | 132.6 KB | Display | |
Data in CIF | 1h0t_validation.cif.gz | 179.4 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/h0/1h0t ftp://data.pdbj.org/pub/pdb/validation_reports/h0/1h0t | HTTPS FTP |
-Related structure data
Related structure data | |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Antibody | Mass: 6648.316 Da / Num. of mol.: 1 / Fragment: RESIDUES 213-269 Source method: isolated from a genetically manipulated source Source: (gene. exp.) STAPHYLOCOCCUS AUREUS (bacteria) / Plasmid: PET28A(+) / Production host: ESCHERICHIA COLI (E. coli) / Strain (production host): BL21(DE3) / References: UniProt: P02976 |
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#2: Protein | Mass: 6447.263 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) SYNTHETIC CONSTRUCT (others) / Plasmid: PET28A(+) / Production host: ESCHERICHIA COLI (E. coli) / Strain (production host): BL21(DE3) |
-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR |
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NMR details | Text: THE STRUCTURE WAS DETERMINED USING TRIPLE-RESONANCE NMR SPECTROSCOPY ON 13C, 15N-LABELED PROTEINS. ONE OF THE SUBUNITS WAS LABELED THE OTHER SUBUNIT WAS KEPT UNLABELED. |
-Sample preparation
Sample conditions | pH: 5.6 / Pressure: 1 atm / Temperature: 303 K |
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Crystal grow | *PLUS Method: other / Details: NMR |
-NMR measurement
NMR spectrometer |
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-Processing
NMR software |
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Refinement | Method: SIMULATED ANEALING / Software ordinal: 1 Details: REFINEMENT DETAILS CAN BE FOUND IN THE JRNL CITATION ABOVE. | ||||||||||||||||||||||||
NMR ensemble | Conformer selection criteria: LEAST RESTRAINT VIOLATION AND GOOD RAMACHANDRAN PLOTS Conformers calculated total number: 100 / Conformers submitted total number: 40 |