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基本情報
登録情報 | データベース: PDB / ID: 1gzo | ||||||
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タイトル | Structure of protein kinase B unphosphorylated | ||||||
![]() | RAC-BETA SERINE/THREONINE PROTEIN KINASE | ||||||
![]() | KINASE / TRANSFERASE / SERINE/THREONINE-PROTEIN KINASE / ATP-BINDING | ||||||
機能・相同性 | ![]() retinal rod cell apoptotic process / PDE3B signalling / cellular response to high light intensity / Inhibition of TSC complex formation by PKB / AKT-mediated inactivation of FOXO1A / Negative regulation of the PI3K/AKT network / negative regulation of long-chain fatty acid import across plasma membrane / Activation of AKT2 / AKT phosphorylates targets in the nucleus / RUNX2 regulates genes involved in cell migration ...retinal rod cell apoptotic process / PDE3B signalling / cellular response to high light intensity / Inhibition of TSC complex formation by PKB / AKT-mediated inactivation of FOXO1A / Negative regulation of the PI3K/AKT network / negative regulation of long-chain fatty acid import across plasma membrane / Activation of AKT2 / AKT phosphorylates targets in the nucleus / RUNX2 regulates genes involved in cell migration / : / positive regulation of fatty acid beta-oxidation / Regulation of MITF-M-dependent genes involved in pigmentation / mammary gland epithelial cell differentiation / RAB GEFs exchange GTP for GDP on RABs / positive regulation of glucose metabolic process / peripheral nervous system myelin maintenance / positive regulation of cell motility / glycogen biosynthetic process / AKT phosphorylates targets in the cytosol / Regulation of TP53 Activity through Association with Co-factors / CTLA4 inhibitory signaling / fat cell differentiation / Constitutive Signaling by AKT1 E17K in Cancer / CD28 dependent PI3K/Akt signaling / Regulation of localization of FOXO transcription factors / positive regulation of glycogen biosynthetic process / Estrogen-dependent nuclear events downstream of ESR-membrane signaling / Activation of BAD and translocation to mitochondria / positive regulation of protein targeting to membrane / SARS-CoV-2 targets host intracellular signalling and regulatory pathways / Cyclin E associated events during G1/S transition / Cyclin A:Cdk2-associated events at S phase entry / Regulation of TP53 Activity through Acetylation / FLT3 Signaling / regulation of cell migration / Downregulation of ERBB2:ERBB3 signaling / VEGFR2 mediated vascular permeability / molecular function activator activity / protein localization to plasma membrane / Translocation of SLC2A4 (GLUT4) to the plasma membrane / Deactivation of the beta-catenin transactivating complex / positive regulation of glucose import / TP53 Regulates Metabolic Genes / protein modification process / ruffle membrane / Regulation of PTEN stability and activity / cellular response to insulin stimulus / G beta:gamma signalling through PI3Kgamma / glucose metabolic process / KEAP1-NFE2L2 pathway / Regulation of TP53 Degradation / insulin receptor signaling pathway / PIP3 activates AKT signaling / regulation of translation / cell cortex / early endosome / regulation of cell cycle / non-specific serine/threonine protein kinase / intracellular signal transduction / positive regulation of cell migration / intracellular membrane-bounded organelle / protein serine kinase activity / protein serine/threonine kinase activity / signal transduction / nucleoplasm / ATP binding / nucleus / metal ion binding / plasma membrane / cytosol 類似検索 - 分子機能 | ||||||
生物種 | ![]() | ||||||
手法 | ![]() ![]() ![]() | ||||||
![]() | Barford, D. / Yang, J. / Hemmings, B.A. | ||||||
![]() | ![]() タイトル: Molecular Mechanism for the Regulation of Protein Kinase B/Akt by Hydrophobic Motif Phosphorylation 著者: Yang, J. / Cron, P. / Thompson, V. / Good, V. / Hess, D. / Hemmings, B.A. / Barford, D. | ||||||
履歴 |
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構造の表示
構造ビューア | 分子: ![]() ![]() |
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PDBx/mmCIF形式 | ![]() | 71.8 KB | 表示 | ![]() |
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PDB形式 | ![]() | 52.2 KB | 表示 | ![]() |
PDBx/mmJSON形式 | ![]() | ツリー表示 | ![]() | |
その他 | ![]() |
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アーカイブディレクトリ | ![]() ![]() | HTTPS FTP |
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-関連構造データ
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リンク
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集合体
登録構造単位 | ![]()
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単位格子 |
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要素
#1: タンパク質 | 分子量: 36490.875 Da / 分子数: 1 断片: KINASE DOMAIN WITHOUT HYDROPHOBIC MOTIF, RESIDUES 146-460 由来タイプ: 組換発現 / 由来: (組換発現) ![]() 発現宿主: ![]() ![]() 参照: UniProt: P31751, 転移酵素; リンを含む基を移すもの; キナーゼ(アルコールにつなげるもの) |
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#2: 水 | ChemComp-HOH / |
構成要素の詳細 | FUNCTION: PROTEIN KINASE PHOSPHORYLATING SEVERAL KNOWN PROTEINS. TISSUE SPECIFICITY: ALL HUMAN CELL ...FUNCTION: PROTEIN KINASE PHOSPHORYL |
-実験情報
-実験
実験 | 手法: ![]() |
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試料調製
結晶 | マシュー密度: 2.6 Å3/Da / 溶媒含有率: 47 % | ||||||||||||||||||||||||||||||||||||||||||
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結晶化 | pH: 7.5 詳細: 30% PEG 8000, 0.2 M LITHIUM SULPHATE, 0.1 M TRIS, 10MG/ML PROTEIN, pH 7.50 | ||||||||||||||||||||||||||||||||||||||||||
結晶化 | *PLUS 温度: 20 ℃ / pH: 8.5 / 手法: batch method | ||||||||||||||||||||||||||||||||||||||||||
溶液の組成 | *PLUS
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-データ収集
回折 | 平均測定温度: 100 K |
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放射光源 | 由来: ![]() ![]() ![]() |
検出器 | タイプ: ADSC CCD / 検出器: CCD / 日付: 2001年9月15日 |
放射 | プロトコル: SINGLE WAVELENGTH / 単色(M)・ラウエ(L): M / 散乱光タイプ: x-ray |
放射波長 | 波長: 0.92 Å / 相対比: 1 |
反射 | 解像度: 2.75→30 Å / Num. obs: 12147 / % possible obs: 94.2 % / Observed criterion σ(I): 0 / 冗長度: 4.2 % / Biso Wilson estimate: 104.6 Å2 / Rmerge(I) obs: 0.062 / Net I/σ(I): 18 |
反射 シェル | 解像度: 2.75→2.85 Å / % possible all: 84 |
反射 | *PLUS 最高解像度: 2.7 Å / 最低解像度: 30 Å / Num. measured all: 50875 / Rmerge(I) obs: 0.065 |
反射 シェル | *PLUS % possible obs: 84 % / Rmerge(I) obs: 0.236 |
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解析
ソフトウェア |
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精密化 | 構造決定の手法: ![]() 開始モデル: PDB ENTRY 1CDK 解像度: 2.75→25.67 Å / Rfactor Rfree error: 0.009 / Data cutoff high absF: 1488479.19 / Isotropic thermal model: RESTRAINED / 交差検証法: THROUGHOUT / σ(F): 0
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溶媒の処理 | 溶媒モデル: FLAT MODEL / Bsol: 45.6653 Å2 / ksol: 0.308053 e/Å3 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
原子変位パラメータ | Biso mean: 57.7 Å2
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Refine analyze |
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精密化ステップ | サイクル: LAST / 解像度: 2.75→25.67 Å
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拘束条件 |
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LS精密化 シェル | 解像度: 2.75→2.92 Å / Rfactor Rfree error: 0.033 / Total num. of bins used: 6
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精密化 | *PLUS 最低解像度: 35 Å / % reflection Rfree: 9.9 % / Rfactor Rfree: 0.3 / Rfactor Rwork: 0.238 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
溶媒の処理 | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
原子変位パラメータ | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
拘束条件 | *PLUS
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