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Open data
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Basic information
Entry | Database: PDB / ID: 1gy8 | ||||||
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Title | Trypanosoma brucei UDP-galactose 4' epimerase | ||||||
![]() | UDP-GALACTOSE 4-EPIMERASE | ||||||
![]() | OXIDOREDUCTASE / EPIMERASE / GALACTOSE / TRYPANOSOMA BRUCEI | ||||||
Function / homology | ![]() UDP-glucose 4-epimerase / UDP-glucose 4-epimerase activity / galactose catabolic process via UDP-galactose / glycosome / nucleotide binding / cytosol Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Shaw, M.P. / Bond, C.S. / Hunter, W.N. | ||||||
![]() | ![]() Title: High-Resolution Crystal Structure of Trypanosoma Brucei Udp-Galactose 4'-Epimerase: A Potential Target for Structure-Based Development of Novel Trypanocides Authors: Shaw, M.P. / Bond, C.S. / Roper, J.R. / Gourley, D.G. / Ferguson, M.A.J. / Hunter, W.N. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 325.9 KB | Display | ![]() |
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PDB format | ![]() | 264.1 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 3 MB | Display | ![]() |
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Full document | ![]() | 3 MB | Display | |
Data in XML | ![]() | 70.4 KB | Display | |
Data in CIF | ![]() | 100.1 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 1ek6S S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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2 | ![]()
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Unit cell |
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Noncrystallographic symmetry (NCS) | NCS oper:
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Components
#1: Protein | Mass: 43806.547 Da / Num. of mol.: 4 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() #2: Chemical | ChemComp-NAD / #3: Chemical | ChemComp-UDP / #4: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.6 Å3/Da / Density % sol: 51.7 % | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Crystal grow | pH: 7 Details: PROTEIN WAS CRYSTALLISED WITH NAD AND UDP, FROM 100M HEPES PH7.0, 14% PEG8000, 200MM KCL, pH 7.00 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Crystal grow | *PLUS pH: 7.5 / Method: vapor diffusion, hanging drop | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: ADSC CCD / Detector: CCD |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.009 Å / Relative weight: 1 |
Reflection | Resolution: 2→20 Å / Num. obs: 112132 / % possible obs: 90.4 % / Redundancy: 3.7 % / Biso Wilson estimate: 25.1 Å2 / Rmerge(I) obs: 0.066 / Net I/σ(I): 9.09 |
Reflection shell | Resolution: 2→2.07 Å / Rmerge(I) obs: 0.361 / Mean I/σ(I) obs: 1.15 / % possible all: 59.3 |
Reflection | *PLUS Num. measured all: 417521 |
Reflection shell | *PLUS Highest resolution: 2 Å / % possible obs: 59.3 % |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: PDB ENTRY 1EK6 Resolution: 2→100 Å / σ(F): 0
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Displacement parameters | Biso mean: 31.6 Å2
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Refinement step | Cycle: LAST / Resolution: 2→100 Å
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Refine LS restraints |
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Refinement | *PLUS Highest resolution: 2 Å / Rfactor Rwork: 0.215 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints | *PLUS Type: c_angle_deg / Dev ideal: 1.284 |