[English] 日本語
![](img/lk-miru.gif)
- PDB-1gqy: MURC - CRYSTAL STRUCTURE OF THE ENZYME FROM HAEMOPHILUS INFLUENZA... -
+
Open data
-
Basic information
Entry | Database: PDB / ID: 1gqy | ||||||
---|---|---|---|---|---|---|---|
Title | MURC - CRYSTAL STRUCTURE OF THE ENZYME FROM HAEMOPHILUS INFLUENZAE COMPLEXED WITH AMPPCP | ||||||
![]() | UDP-N-ACETYLMURAMATE-L-ALANINE LIGASE | ||||||
![]() | CELL WALL BIOSYNTHESIS / PEPTIDOGLYCAN / MUREIN / LIGASE / ATP BINDING | ||||||
Function / homology | ![]() UDP-N-acetylmuramate-L-alanine ligase / UDP-N-acetylmuramate-L-alanine ligase activity / peptidoglycan biosynthetic process / cell wall organization / regulation of cell shape / cell cycle / cell division / ATP binding / cytoplasm Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Skarzynski, T. / Cleasby, A. / Domenici, E. / Gevi, M. / Shaw, J. | ||||||
![]() | ![]() Title: Crystal Structures of Udp-N-Acetylmuramate-L-Alanine Ligase (Murc) from Haemophilus Influenzae Authors: Skarzynski, T. / Cleasby, A. / Domenici, E. / Gevi, M. / Shaw, J. | ||||||
History |
|
-
Structure visualization
Structure viewer | Molecule: ![]() ![]() |
---|
-
Downloads & links
-
Download
PDBx/mmCIF format | ![]() | 209.8 KB | Display | ![]() |
---|---|---|---|---|
PDB format | ![]() | 167.2 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 501 KB | Display | ![]() |
---|---|---|---|---|
Full document | ![]() | 369.5 KB | Display | |
Data in XML | ![]() | 19.2 KB | Display | |
Data in CIF | ![]() | 35.3 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 1gqqSC S: Starting model for refinement C: citing same article ( |
---|---|
Similar structure data |
-
Links
-
Assembly
Deposited unit | ![]()
| ||||||||
---|---|---|---|---|---|---|---|---|---|
1 |
| ||||||||
Unit cell |
|
-
Components
#1: Protein | Mass: 52057.172 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() References: UniProt: P45066, UDP-N-acetylmuramate-L-alanine ligase #2: Chemical | #3: Chemical | #4: Water | ChemComp-HOH / | |
---|
-Experimental details
-Experiment
Experiment | Method: ![]() |
---|
-
Sample preparation
Crystal | Density Matthews: 2.73 Å3/Da / Density % sol: 54.89 % |
---|---|
Crystal grow | pH: 6.8 Details: THE AMPPCP COMPLEX WAS FORMED BY ADDING 10MM AMPPCP AND 10MM MGCL2 TO THE CONCENTRATED PROTEIN SOLUTION AT 12MG/ML. CRYSTALS WERE GROWN USING WELL SOLUTION MADE OF 20%PEG 3350 AND 200MM SODIUM FORMATE, PH 6.8. |
-Data collection
Diffraction | Mean temperature: 100 K |
---|---|
Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: ADSC CCD / Detector: CCD / Date: Oct 7, 1999 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.87 Å / Relative weight: 1 |
Reflection | Resolution: 1.8→30 Å / Num. obs: 93715 / % possible obs: 89.5 % / Redundancy: 2 % / Rmerge(I) obs: 0.069 |
Reflection shell | Resolution: 1.8→1.86 Å / Rmerge(I) obs: 0.453 / % possible all: 47.7 |
-
Processing
Software |
| ||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Refinement | Method to determine structure: ![]() Starting model: AN EARLY VERSION OF PDB ENTRY 1GQQ Resolution: 1.8→20 Å / SU B: 3.26087 / SU ML: 0.09953 / Cross valid method: THROUGHOUT / σ(F): 0 / ESU R: 0.12872 / ESU R Free: 0.12945 Details: DISORDERED SIDE CHAINS WERE MODELLED STEREOCHEMICALLY
| ||||||||||||||||||||
Displacement parameters | Biso mean: 30.035 Å2
| ||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.8→20 Å
|