[English] 日本語
Yorodumi- PDB-1gnf: SOLUTION STRUCTURE OF THE N-TERMINAL ZINC FINGER OF MURINE GATA-1... -
+Open data
-Basic information
Entry | Database: PDB / ID: 1gnf | ||||||
---|---|---|---|---|---|---|---|
Title | SOLUTION STRUCTURE OF THE N-TERMINAL ZINC FINGER OF MURINE GATA-1, NMR, 25 STRUCTURES | ||||||
Components | TRANSCRIPTION FACTOR GATA-1 | ||||||
Keywords | TRANSCRIPTION REGULATION / ZINC FINGER | ||||||
Function / homology | Function and homology information regulation of primitive erythrocyte differentiation / basophil differentiation / eosinophil fate commitment / regulation of definitive erythrocyte differentiation / megakaryocyte differentiation / regulation of glycoprotein biosynthetic process / myeloid cell apoptotic process / primitive erythrocyte differentiation / osteoblast proliferation / cell development ...regulation of primitive erythrocyte differentiation / basophil differentiation / eosinophil fate commitment / regulation of definitive erythrocyte differentiation / megakaryocyte differentiation / regulation of glycoprotein biosynthetic process / myeloid cell apoptotic process / primitive erythrocyte differentiation / osteoblast proliferation / cell development / Sertoli cell development / RUNX1 regulates genes involved in megakaryocyte differentiation and platelet function / RUNX1 regulates transcription of genes involved in differentiation of HSCs / negative regulation of bone mineralization / dendritic cell differentiation / cellular response to follicle-stimulating hormone stimulus / Factors involved in megakaryocyte development and platelet production / positive regulation of mast cell degranulation / negative regulation of myeloid cell apoptotic process / myeloid cell differentiation / C2H2 zinc finger domain binding / cellular response to thyroid hormone stimulus / embryonic hemopoiesis / platelet formation / erythrocyte development / bone mineralization / positive regulation of osteoblast proliferation / negative regulation of extrinsic apoptotic signaling pathway in absence of ligand / cell fate commitment / animal organ regeneration / cis-regulatory region sequence-specific DNA binding / homeostasis of number of cells within a tissue / cellular response to cAMP / transcription repressor complex / erythrocyte differentiation / positive regulation of erythrocyte differentiation / transcription coregulator binding / protein-DNA complex / RNA polymerase II transcription regulatory region sequence-specific DNA binding / chromatin DNA binding / platelet aggregation / transcription coactivator binding / male gonad development / positive regulation of peptidyl-tyrosine phosphorylation / p53 binding / sequence-specific double-stranded DNA binding / cell-cell signaling / positive regulation of cytosolic calcium ion concentration / DNA-binding transcription activator activity, RNA polymerase II-specific / cell population proliferation / in utero embryonic development / cellular response to lipopolysaccharide / RNA polymerase II-specific DNA-binding transcription factor binding / transcription regulator complex / sequence-specific DNA binding / transcription by RNA polymerase II / transcription cis-regulatory region binding / DNA-binding transcription factor activity, RNA polymerase II-specific / RNA polymerase II cis-regulatory region sequence-specific DNA binding / DNA-binding transcription factor activity / negative regulation of cell population proliferation / chromatin binding / negative regulation of apoptotic process / positive regulation of DNA-templated transcription / negative regulation of transcription by RNA polymerase II / positive regulation of transcription by RNA polymerase II / DNA binding / zinc ion binding / nucleoplasm / nucleus Similarity search - Function | ||||||
Biological species | Mus musculus (house mouse) | ||||||
Method | SOLUTION NMR / DISTANCE GEOMETRY, SIMULATED ANNEALING | ||||||
Authors | Kowalski, K. / Czolij, R. / King, G.F. / Crossley, M. / Mackay, J.P. | ||||||
Citation | Journal: J.Biomol.NMR / Year: 1999 Title: The solution structure of the N-terminal zinc finger of GATA-1 reveals a specific binding face for the transcriptional co-factor FOG. Authors: Kowalski, K. / Czolij, R. / King, G.F. / Crossley, M. / Mackay, J.P. | ||||||
History |
|
-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
---|
-Downloads & links
-Download
PDBx/mmCIF format | 1gnf.cif.gz | 349 KB | Display | PDBx/mmCIF format |
---|---|---|---|---|
PDB format | pdb1gnf.ent.gz | 299.5 KB | Display | PDB format |
PDBx/mmJSON format | 1gnf.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/gn/1gnf ftp://data.pdbj.org/pub/pdb/validation_reports/gn/1gnf | HTTPS FTP |
---|
-Related structure data
Similar structure data |
---|
-Links
-Assembly
Deposited unit |
| |||||||||
---|---|---|---|---|---|---|---|---|---|---|
1 |
| |||||||||
NMR ensembles |
|
-Components
#1: Protein/peptide | Mass: 5199.939 Da / Num. of mol.: 1 / Fragment: N-TERMINAL ZINC FINGER Source method: isolated from a genetically manipulated source Source: (gene. exp.) Mus musculus (house mouse) / Cell line: BL21 / Plasmid: PGEX-2T / Species (production host): Escherichia coli / Production host: Escherichia coli BL21(DE3) (bacteria) / Strain (production host): BL21 (DE3) / References: UniProt: P17679 |
---|---|
#2: Chemical | ChemComp-ZN / |
-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
NMR experiment |
| ||||||||||||||||||||||||||||||||||||
NMR details | Text: THE STRUCTURE WAS DETERMINED USING HOMONUCLEAR 1H, AND HETERONUCLEAR 1H/15N NMR SPECTROSCOPY |
-Sample preparation
Details | Contents: 95% H2O:5% D2O |
---|---|
Sample conditions | pH: 5.4 / Pressure: 1 atm / Temperature: 288 K |
Crystal grow | *PLUS Method: other / Details: NMR |
-NMR measurement
NMR spectrometer |
|
---|
-Processing
Software |
| ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
NMR software |
| ||||||||||||||||
Refinement | Method: DISTANCE GEOMETRY, SIMULATED ANNEALING / Software ordinal: 1 Details: REFINEMENT DETAILS CAN BE FOUND IN THE JRNL CITATION ABOVE | ||||||||||||||||
NMR ensemble | Conformer selection criteria: LOWEST RESIDUAL RESTRAINT VIOLATIONS Conformers calculated total number: 1000 / Conformers submitted total number: 25 |