+データを開く
-基本情報
登録情報 | データベース: PDB / ID: 1gnf | ||||||
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タイトル | SOLUTION STRUCTURE OF THE N-TERMINAL ZINC FINGER OF MURINE GATA-1, NMR, 25 STRUCTURES | ||||||
要素 | TRANSCRIPTION FACTOR GATA-1 | ||||||
キーワード | TRANSCRIPTION REGULATION / ZINC FINGER (ジンクフィンガー) | ||||||
機能・相同性 | 機能・相同性情報 regulation of primitive erythrocyte differentiation / basophil differentiation / eosinophil fate commitment / regulation of definitive erythrocyte differentiation / megakaryocyte differentiation / regulation of glycoprotein biosynthetic process / myeloid cell apoptotic process / primitive erythrocyte differentiation / osteoblast proliferation / 細胞生物学 ...regulation of primitive erythrocyte differentiation / basophil differentiation / eosinophil fate commitment / regulation of definitive erythrocyte differentiation / megakaryocyte differentiation / regulation of glycoprotein biosynthetic process / myeloid cell apoptotic process / primitive erythrocyte differentiation / osteoblast proliferation / 細胞生物学 / Sertoli cell development / RUNX1 regulates transcription of genes involved in differentiation of HSCs / RUNX1 regulates genes involved in megakaryocyte differentiation and platelet function / negative regulation of bone mineralization / dendritic cell differentiation / cellular response to follicle-stimulating hormone stimulus / Factors involved in megakaryocyte development and platelet production / positive regulation of mast cell degranulation / negative regulation of myeloid cell apoptotic process / myeloid cell differentiation / C2H2 zinc finger domain binding / embryonic hemopoiesis / platelet formation / erythrocyte development / bone mineralization / positive regulation of osteoblast proliferation / negative regulation of extrinsic apoptotic signaling pathway in absence of ligand / 細胞分化 / animal organ regeneration / cis-regulatory region sequence-specific DNA binding / homeostasis of number of cells within a tissue / cellular response to cAMP / transcription repressor complex / 赤血球形成 / positive regulation of erythrocyte differentiation / transcription coregulator binding / デオキシリボ核酸 / RNA polymerase II transcription regulatory region sequence-specific DNA binding / transcription coactivator binding / chromatin DNA binding / 血小板 / male gonad development / positive regulation of peptidyl-tyrosine phosphorylation / sequence-specific double-stranded DNA binding / p53 binding / cell-cell signaling / positive regulation of cytosolic calcium ion concentration / DNA-binding transcription activator activity, RNA polymerase II-specific / cellular response to lipopolysaccharide / cell population proliferation / in utero embryonic development / transcription regulator complex / RNA polymerase II-specific DNA-binding transcription factor binding / sequence-specific DNA binding / transcription by RNA polymerase II / transcription cis-regulatory region binding / DNA-binding transcription factor activity, RNA polymerase II-specific / RNA polymerase II cis-regulatory region sequence-specific DNA binding / DNA-binding transcription factor activity / negative regulation of cell population proliferation / chromatin binding / negative regulation of apoptotic process / positive regulation of DNA-templated transcription / negative regulation of transcription by RNA polymerase II / positive regulation of transcription by RNA polymerase II / DNA binding / zinc ion binding / 核質 / 細胞核 類似検索 - 分子機能 | ||||||
生物種 | Mus musculus (ハツカネズミ) | ||||||
手法 | 溶液NMR / DISTANCE GEOMETRY, SIMULATED ANNEALING | ||||||
データ登録者 | Kowalski, K. / Czolij, R. / King, G.F. / Crossley, M. / Mackay, J.P. | ||||||
引用 | ジャーナル: J.Biomol.NMR / 年: 1999 タイトル: The solution structure of the N-terminal zinc finger of GATA-1 reveals a specific binding face for the transcriptional co-factor FOG. 著者: Kowalski, K. / Czolij, R. / King, G.F. / Crossley, M. / Mackay, J.P. | ||||||
履歴 |
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-構造の表示
構造ビューア | 分子: MolmilJmol/JSmol |
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-ダウンロードとリンク
-ダウンロード
PDBx/mmCIF形式 | 1gnf.cif.gz | 349 KB | 表示 | PDBx/mmCIF形式 |
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PDB形式 | pdb1gnf.ent.gz | 299.5 KB | 表示 | PDB形式 |
PDBx/mmJSON形式 | 1gnf.json.gz | ツリー表示 | PDBx/mmJSON形式 | |
その他 | その他のダウンロード |
-検証レポート
アーカイブディレクトリ | https://data.pdbj.org/pub/pdb/validation_reports/gn/1gnf ftp://data.pdbj.org/pub/pdb/validation_reports/gn/1gnf | HTTPS FTP |
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-関連構造データ
-リンク
-集合体
登録構造単位 |
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1 |
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NMR アンサンブル |
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-要素
#1: タンパク質・ペプチド | 分子量: 5199.939 Da / 分子数: 1 / 断片: N-TERMINAL ZINC FINGER / 由来タイプ: 組換発現 / 由来: (組換発現) Mus musculus (ハツカネズミ) / 細胞株: BL21 / プラスミド: PGEX-2T / 生物種 (発現宿主): Escherichia coli / 発現宿主: Escherichia coli BL21(DE3) (大腸菌) / 株 (発現宿主): BL21 (DE3) / 参照: UniProt: P17679 |
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#2: 化合物 | ChemComp-ZN / |
-実験情報
-実験
実験 | 手法: 溶液NMR | ||||||||||||||||||||||||||||||||||||
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NMR実験 |
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NMR実験の詳細 | Text: THE STRUCTURE WAS DETERMINED USING HOMONUCLEAR 1H, AND HETERONUCLEAR 1H/15N NMR SPECTROSCOPY |
-試料調製
詳細 | 内容: 95% H2O:5% D2O |
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試料状態 | pH: 5.4 / 圧: 1 atm / 温度: 288 K |
結晶化 | *PLUS 手法: その他 / 詳細: NMR |
-NMR測定
NMRスペクトロメーター |
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-解析
ソフトウェア |
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NMR software |
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精密化 | 手法: DISTANCE GEOMETRY, SIMULATED ANNEALING / ソフトェア番号: 1 詳細: REFINEMENT DETAILS CAN BE FOUND IN THE JRNL CITATION ABOVE | ||||||||||||||||
NMRアンサンブル | コンフォーマー選択の基準: LOWEST RESIDUAL RESTRAINT VIOLATIONS 計算したコンフォーマーの数: 1000 / 登録したコンフォーマーの数: 25 |