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- PDB-1gl8: Solution structure of thioredoxin m from spinach, oxidized form -

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Basic information

Entry
Database: PDB / ID: 1gl8
TitleSolution structure of thioredoxin m from spinach, oxidized form
ComponentsTHIOREDOXIN
KeywordsOXIDOREDUCTASE / ELECTRON TRANSPORT / REDOX-ACTIVE CENTER
Function / homology
Function and homology information


protein-disulfide reductase activity / enzyme activator activity / chloroplast
Similarity search - Function
Thioredoxin / Thioredoxin / Thioredoxin, conserved site / Thioredoxin family active site. / Thioredoxin domain profile. / Thioredoxin domain / Glutaredoxin / Glutaredoxin / Thioredoxin-like superfamily / 3-Layer(aba) Sandwich / Alpha Beta
Similarity search - Domain/homology
Thioredoxin M-type, chloroplastic
Similarity search - Component
Biological speciesSPINACIA OLERACEA (spinach)
MethodSOLUTION NMR / RESTRAINED MOLECULAR DYNAMICS
AuthorsNeira, J.L. / Gonzalez, C. / Toiron, C. / De-Prat-gay, G. / Rico, M.
CitationJournal: Biochemistry / Year: 2001
Title: Three-Dimensional Solution Structure and Stability of Thioredoxin M from Spinach.
Authors: Neira, J.L. / Gonzalez, C. / Toiron, C. / De Prat-Gay, G. / Rico, M.
History
DepositionAug 30, 2001Deposition site: PDBE / Processing site: PDBE
Revision 1.0Dec 13, 2001Provider: repository / Type: Initial release
Revision 1.1May 8, 2011Group: Version format compliance
Revision 1.2Jul 13, 2011Group: Version format compliance

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

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Assembly

Deposited unit
A: THIOREDOXIN


Theoretical massNumber of molelcules
Total (without water)11,6641
Polymers11,6641
Non-polymers00
Water00
1


  • Idetical with deposited unit
  • defined by author
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
NMR ensembles
DataCriteria
Number of conformers (submitted / calculated)25 / 25
Representative

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Components

#1: Protein THIOREDOXIN


Mass: 11664.322 Da / Num. of mol.: 1 / Fragment: OXIDISED FORM, RESIDUES 14-117
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) SPINACIA OLERACEA (spinach) / Production host: ESCHERICHIA COLI (E. coli) / References: UniProt: P07591

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Experimental details

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Experiment

ExperimentMethod: SOLUTION NMR
NMR experiment
Conditions-IDExperiment-IDSolution-IDType
111NOESY
121TOCSY
131COSY

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Sample preparation

Sample conditionspH: 5.8 / Pressure: 1 atm / Temperature: 308 K
Crystal grow
*PLUS
Method: other / Details: NMR

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NMR measurement

NMR spectrometerType: Bruker AMX / Manufacturer: Bruker / Model: AMX / Field strength: 600 MHz

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Processing

NMR software
NameDeveloperClassification
GROMOSVAN GUNSTEREN,BERENDSENrefinement
GROMOSstructure solution
RefinementMethod: RESTRAINED MOLECULAR DYNAMICS / Software ordinal: 1
Details: REFINEMENT DETAILS CAN BE FOUND IN THE JRNL CITATION ABOVE
NMR ensembleConformers calculated total number: 25 / Conformers submitted total number: 25

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