+Open data
-Basic information
Entry | Database: PDB / ID: 1gk5 | ||||||
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Title | Solution Structure the mEGF/TGFalpha44-50 chimeric growth factor | ||||||
Components | Pro-epidermal growth factor,Protransforming growth factor alpha | ||||||
Keywords | GROWTH FACTOR / EGF GROWTH FACTOR / CHIMERIC | ||||||
Function / homology | Function and homology information Signaling by ERBB4 / EGFR interacts with phospholipase C-gamma / ERBB2 Activates PTK6 Signaling / Signaling by EGFR / PI3K events in ERBB2 signaling / SHC1 events in ERBB2 signaling / GRB2 events in EGFR signaling / SHC1 events in EGFR signaling / Signaling by ERBB2 / GAB1 signalosome ...Signaling by ERBB4 / EGFR interacts with phospholipase C-gamma / ERBB2 Activates PTK6 Signaling / Signaling by EGFR / PI3K events in ERBB2 signaling / SHC1 events in ERBB2 signaling / GRB2 events in EGFR signaling / SHC1 events in EGFR signaling / Signaling by ERBB2 / GAB1 signalosome / ERBB2 Regulates Cell Motility / NOTCH3 Activation and Transmission of Signal to the Nucleus / Downregulation of ERBB2 signaling / EGFR downregulation / negative regulation of secretion / regulation of protein transport / negative regulation of cholesterol efflux / positive regulation of hyaluronan biosynthetic process / hepatocyte proliferation / Extra-nuclear estrogen signaling / positive regulation of cerebellar granule cell precursor proliferation / RAF/MAP kinase cascade / cerebellar granule cell precursor proliferation / PIP3 activates AKT signaling / positive regulation of epithelial tube formation / Platelet degranulation / positive regulation of protein localization to early endosome / Cargo recognition for clathrin-mediated endocytosis / regulation of calcium ion import / regulation of protein localization to cell surface / PI5P, PP2A and IER3 Regulate PI3K/AKT Signaling / Clathrin-mediated endocytosis / positive regulation of ubiquitin-dependent protein catabolic process / Cargo concentration in the ER / regulation of receptor signaling pathway via JAK-STAT / COPII-mediated vesicle transport / Inhibition of Signaling by Overexpressed EGFR / EGFR interacts with phospholipase C-gamma / ERBB2-EGFR signaling pathway / epidermal growth factor receptor binding / positive regulation of DNA biosynthetic process / branching morphogenesis of an epithelial tube / positive regulation of DNA binding / Signaling by EGFR / transmembrane receptor protein tyrosine kinase activator activity / positive regulation of receptor internalization / positive regulation of cell division / Estrogen-dependent nuclear events downstream of ESR-membrane signaling / mammary gland alveolus development / GAB1 signalosome / regulation of peptidyl-tyrosine phosphorylation / positive regulation of endothelial cell proliferation / GRB2 events in EGFR signaling / SHC1 events in EGFR signaling / endoplasmic reticulum-Golgi intermediate compartment membrane / TFAP2 (AP-2) family regulates transcription of growth factors and their receptors / ERK1 and ERK2 cascade / positive regulation of endothelial cell migration / positive regulation of mitotic nuclear division / positive regulation of epithelial cell proliferation / epithelial cell proliferation / guanyl-nucleotide exchange factor activity / positive regulation of peptidyl-threonine phosphorylation / EGFR downregulation / growth factor activity / clathrin-coated endocytic vesicle membrane / epidermal growth factor receptor signaling pathway / ER to Golgi transport vesicle membrane / Constitutive Signaling by Aberrant PI3K in Cancer / positive regulation of peptidyl-tyrosine phosphorylation / positive regulation of fibroblast proliferation / positive regulation of canonical Wnt signaling pathway / Cargo recognition for clathrin-mediated endocytosis / PIP3 activates AKT signaling / Clathrin-mediated endocytosis / PI5P, PP2A and IER3 Regulate PI3K/AKT Signaling / RAF/MAP kinase cascade / cytoplasmic vesicle / basolateral plasma membrane / angiogenesis / Estrogen-dependent gene expression / cell population proliferation / positive regulation of MAPK cascade / Extra-nuclear estrogen signaling / positive regulation of ERK1 and ERK2 cascade / positive regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction / cell surface receptor signaling pathway / intracellular signal transduction / receptor ligand activity / positive regulation of cell population proliferation / calcium ion binding / endoplasmic reticulum membrane / positive regulation of gene expression / positive regulation of DNA-templated transcription / perinuclear region of cytoplasm / cell surface / extracellular space / extracellular exosome / extracellular region / plasma membrane Similarity search - Function | ||||||
Biological species | Mus musculus (house mouse) Homo sapiens (human) | ||||||
Method | SOLUTION NMR / TORSION ANGLE DYNAMICS WITH SIMULATED ANNEALING | ||||||
Authors | Chamberlin, S.G. / Brennan, L. / Puddicombe, S.M. / Davies, D.E. / Turner, D.L. | ||||||
Citation | Journal: Eur.J.Biochem. / Year: 2001 Title: Solution Structure of the Megf/Tgfalpha44-50 Chimeric Growth Factor. Authors: Chamberlin, S. / Brennan, L. / Puddicombe, S. / Davies, D. / Turner, D. #1: Journal: J.Biol.Chem. / Year: 1996 Title: The Interaction of an Epidermal Growth Factor/ Transforming Growth Factor Alpha Tail Chimera with the Human Epidermal Growth Factor Receptor Reveals Unexpected Complexities Authors: Puddicombe, S.M. / Wood, L. / Chamberlin, S.G. / Davies, D.E. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1gk5.cif.gz | 136.2 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1gk5.ent.gz | 116.3 KB | Display | PDB format |
PDBx/mmJSON format | 1gk5.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 1gk5_validation.pdf.gz | 340.7 KB | Display | wwPDB validaton report |
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Full document | 1gk5_full_validation.pdf.gz | 397.3 KB | Display | |
Data in XML | 1gk5_validation.xml.gz | 9.9 KB | Display | |
Data in CIF | 1gk5_validation.cif.gz | 14.5 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/gk/1gk5 ftp://data.pdbj.org/pub/pdb/validation_reports/gk/1gk5 | HTTPS FTP |
-Related structure data
Similar structure data | |
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Other databases |
-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein/peptide | Mass: 5257.785 Da / Num. of mol.: 1 / Fragment: UNP residues 977-1018,UNP residues 83-89 Source method: isolated from a genetically manipulated source Details: THE PROTEIN IS A CHIMERA OF EPIDERMAL GROWTH FACTOR RESIDUES 977-1018 AND HUMAN TRANSFORMING GROWTH FACTOR ALPHA RESIDUES 83-89,THE PROTEIN IS A CHIMERA OF EPIDERMAL GROWTH FACTOR RESIDUES ...Details: THE PROTEIN IS A CHIMERA OF EPIDERMAL GROWTH FACTOR RESIDUES 977-1018 AND HUMAN TRANSFORMING GROWTH FACTOR ALPHA RESIDUES 83-89,THE PROTEIN IS A CHIMERA OF EPIDERMAL GROWTH FACTOR RESIDUES 977-1018 AND HUMAN TRANSFORMING GROWTH FACTOR ALPHA RESIDUES 83-89 Source: (gene. exp.) Mus musculus (house mouse), (gene. exp.) Homo sapiens (human) Gene: Egf, TGFA / Production host: Komagataella pastoris (fungus) / References: UniProt: P01132, UniProt: P01135 |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR |
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NMR experiment | Type: 2D-1H-NOESY 2D-1H-TOCSY 2D-1H-COSY |
-Sample preparation
Sample conditions | pH: 3 / Temperature: 298 K |
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Crystal grow | *PLUS Method: other / Details: NMR |
-NMR measurement
NMR spectrometer | Type: Varian VXR500 / Manufacturer: Varian / Model: VXR500 / Field strength: 500 MHz |
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-Processing
NMR software |
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Refinement | Method: TORSION ANGLE DYNAMICS WITH SIMULATED ANNEALING / Software ordinal: 1 | |||||||||
NMR ensemble | Conformer selection criteria: LEAST RESTRAINT VIOLATION / Conformers calculated total number: 50 / Conformers submitted total number: 10 |