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データを開く
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基本情報
登録情報 | データベース: PDB / ID: 1ghh | |||||||||
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タイトル | SOLUTION STRUCTURE OF DINI | |||||||||
![]() | DNA-DAMAGE-INDUCIBLE PROTEIN I | |||||||||
![]() | PROTEIN BINDING / bicelle / DinI / dipolar coupling / liquid crystal / Pf1 / RecA | |||||||||
機能・相同性 | ![]() | |||||||||
生物種 | ![]() ![]() | |||||||||
手法 | 溶液NMR / simulated annealing, molecular dynamics in Cartesian space | |||||||||
![]() | Ramirez, B.E. / Voloshin, O.N. / Camerini-Otero, R.D. / Bax, A. | |||||||||
![]() | ![]() タイトル: Solution structure of DinI provides insight into its mode of RecA inactivation. 著者: Ramirez, B.E. / Voloshin, O.N. / Camerini-Otero, R.D. / Bax, A. | |||||||||
履歴 |
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構造の表示
構造ビューア | 分子: ![]() ![]() |
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ダウンロードとリンク
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ダウンロード
PDBx/mmCIF形式 | ![]() | 482.1 KB | 表示 | ![]() |
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PDB形式 | ![]() | 404.9 KB | 表示 | ![]() |
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その他 | ![]() |
-検証レポート
アーカイブディレクトリ | ![]() ![]() | HTTPS FTP |
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-関連構造データ
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リンク
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集合体
登録構造単位 | ![]()
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NMR アンサンブル |
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要素
#1: タンパク質 | 分子量: 8957.937 Da / 分子数: 1 / 由来タイプ: 組換発現 / 由来: (組換発現) ![]() ![]() ![]() ![]() |
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-実験情報
-実験
実験 | 手法: 溶液NMR | ||||||||||||||||||||||||||||||||||||
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NMR実験 |
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NMR実験の詳細 | Text: This structure was determined using two sets of dipolar coupling restraints. One set was recorded in a bicelle liquid crystal solution; the other set was recorded in a phage liquid crystal ...Text: This structure was determined using two sets of dipolar coupling restraints. One set was recorded in a bicelle liquid crystal solution; the other set was recorded in a phage liquid crystal solution. See citation 1 for details on solution conditions. A total of 127 N-H, 135 CA-HA, 138 CA-CO, 61 N-CO, and 64 CO-HN dipolar restraints were used in the structure calculation. Additional restraints included 592 intraresidue, 278 short range, 104 medium range, and 140 long range NOE restraints as well as 76 phi, 51 psi, and 21 chi1 dihedral restraints. A conformational database was employed in the simulated annealing calculation. No radius of gyration term was employed in the simulated annealing calculation. |
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試料調製
詳細 |
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試料状態 |
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結晶化 | *PLUS 手法: other / 詳細: NMR |
-NMR測定
NMRスペクトロメーター |
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解析
NMR software |
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精密化 | 手法: simulated annealing, molecular dynamics in Cartesian space ソフトェア番号: 1 詳細: Structures were calculated in a three stage process. In the first stage, folds were calculated from a fully-extended chain based only on torsion and NOE restraints. In the second stage, the ...詳細: Structures were calculated in a three stage process. In the first stage, folds were calculated from a fully-extended chain based only on torsion and NOE restraints. In the second stage, the ten best structures of stage 1 were used as starting structures in a simulated annealing calculation that included dipolar restraints measured in bicelles. In the last stage, the ten lowest energy structures of Stage 2 were used as starting structures in a simulated annealing calculation that also included dipolar restraints measured in a phage liquid crystal. | |||||||||||||||
NMRアンサンブル | コンフォーマー選択の基準: structures with the lowest energy 計算したコンフォーマーの数: 30 / 登録したコンフォーマーの数: 20 |