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Open data
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Basic information
Entry | Database: PDB / ID: 1g66 | ||||||
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Title | ACETYLXYLAN ESTERASE AT 0.90 ANGSTROM RESOLUTION | ||||||
![]() | ACETYL XYLAN ESTERASE II | ||||||
![]() | HYDROLASE / serine hydrolase / acetyl xylopyranose / xylan | ||||||
Function / homology | ![]() acetylxylan esterase / acetylxylan esterase activity / xylan catabolic process / cellulose catabolic process / extracellular region Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() | ||||||
![]() | Ghosh, D. / Sawicki, M. / Lala, P. / Erman, M. / Pangborn, W. / Eyzaguirre, J. / Gutierrez, R. / Jornvall, H. / Thiel, D.J. | ||||||
![]() | ![]() Title: Multiple conformations of catalytic serine and histidine in acetylxylan esterase at 0.90 A. Authors: Ghosh, D. / Sawicki, M. / Lala, P. / Erman, M. / Pangborn, W. / Eyzaguirre, J. / Gutierrez, R. / Jornvall, H. / Thiel, D.J. #1: ![]() Title: Determination of a protein structure by iodination: the structure of iodinated acetylxylan esterase Authors: Ghosh, D. / Erman, M. / Sawicki, M. / Lala, P. / Weeks, D.R. / Li, N. / Pangborn, W. / Thiel, D.J. / Jornvall, H. / Gutierrez, R. / Eyzaguirre, J. #2: ![]() Title: Characterization of crystals of P. purpurogenum acetyl xylan esterase from high resolution X-ray diffraction | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 124.6 KB | Display | ![]() |
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PDB format | ![]() | 104.9 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 388.6 KB | Display | ![]() |
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Full document | ![]() | 391.3 KB | Display | |
Data in XML | ![]() | 6.9 KB | Display | |
Data in CIF | ![]() | 11.6 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 20661.803 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) ![]() | ||||
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#2: Chemical | ChemComp-SO4 / #3: Chemical | ChemComp-GOL / #4: Water | ChemComp-HOH / | |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 1.79 Å3/Da / Density % sol: 31.18 % | ||||||||||||||||||||||||||||||
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Crystal grow | Temperature: 298 K / Method: vapor diffusion / pH: 5.3 Details: Ammonium sulfate, pH 5.3, VAPOR DIFFUSION, temperature 298K | ||||||||||||||||||||||||||||||
Crystal grow | *PLUS Method: vapor diffusion, hanging dropDetails: Pangborn, W., (1996) Proteons Struct.Funct.Genet., 24, 523. | ||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 85 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: PRINCETON 2K / Detector: CCD / Date: Dec 15, 1995 |
Radiation | Monochromator: Sagitally focussed Si (111) / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Relative weight: 1 |
Reflection | Resolution: 0.9→99 Å / Num. all: 95283 / Num. obs: 95283 / % possible obs: 86.6 % / Observed criterion σ(F): 0 / Observed criterion σ(I): 0 / Redundancy: 4.41 % / Biso Wilson estimate: 10 Å2 / Rmerge(I) obs: 0.058 / Net I/σ(I): 29.2 |
Reflection shell | Resolution: 0.9→0.94 Å / Redundancy: 2 % / Rmerge(I) obs: 0.185 / % possible all: 40.4 |
Reflection | *PLUS Num. measured all: 420882 |
Reflection shell | *PLUS % possible obs: 40.4 % |
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Processing
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Refinement | Method to determine structure: AB INITIO PHASING / Resolution: 0.9→99 Å / σ(F): 0 / σ(I): 0 / Stereochemistry target values: Engh & Huber
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Refine analyze | Luzzati coordinate error obs: 0.1 Å | ||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 0.9→99 Å
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Refine LS restraints |
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Software | *PLUS Name: SHELXL-97 / Classification: refinement | ||||||||||||||||||||
Refinement | *PLUS Rfactor obs: 0.103 | ||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||
Displacement parameters | *PLUS | ||||||||||||||||||||
Refine LS restraints | *PLUS
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