+Open data
-Basic information
Entry | Database: PDB / ID: 1g08 | ||||||
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Title | CARBONMONOXY LIGANDED BOVINE HEMOGLOBIN PH 5.0 | ||||||
Components |
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Keywords | OXYGEN STORAGE/TRANSPORT / bovine / hemoglobin / liganded / carbonmonoxy / protoporphyrin IX / OXYGEN STORAGE-TRANSPORT COMPLEX | ||||||
Function / homology | Function and homology information Scavenging of heme from plasma / Heme signaling / Erythrocytes take up carbon dioxide and release oxygen / Erythrocytes take up oxygen and release carbon dioxide / Cytoprotection by HMOX1 / Neutrophil degranulation / hemoglobin alpha binding / haptoglobin-hemoglobin complex / organic acid binding / cellular oxidant detoxification ...Scavenging of heme from plasma / Heme signaling / Erythrocytes take up carbon dioxide and release oxygen / Erythrocytes take up oxygen and release carbon dioxide / Cytoprotection by HMOX1 / Neutrophil degranulation / hemoglobin alpha binding / haptoglobin-hemoglobin complex / organic acid binding / cellular oxidant detoxification / hemoglobin complex / hydrogen peroxide catabolic process / oxygen carrier activity / oxygen binding / iron ion binding / heme binding / metal ion binding Similarity search - Function | ||||||
Biological species | Bos taurus (cattle) | ||||||
Method | X-RAY DIFFRACTION / Resolution: 1.9 Å | ||||||
Authors | Mueser, T.C. / Rogers, P.H. / Arnone, A. | ||||||
Citation | Journal: Biochemistry / Year: 2000 Title: Interface sliding as illustrated by the multiple quaternary structures of liganded hemoglobin. Authors: Mueser, T.C. / Rogers, P.H. / Arnone, A. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1g08.cif.gz | 125 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1g08.ent.gz | 100 KB | Display | PDB format |
PDBx/mmJSON format | 1g08.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/g0/1g08 ftp://data.pdbj.org/pub/pdb/validation_reports/g0/1g08 | HTTPS FTP |
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-Related structure data
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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Details | The biological assembly is a tetramer constructed from the heterodimer of chain A (alpha) and chain B (beta) and the heterodimer of chain C (alpha) and chain D (beta) |
-Components
#1: Protein | Mass: 15077.171 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Bos taurus (cattle) / Tissue: BLOOD / References: UniProt: P01966 #2: Protein | Mass: 15977.382 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Bos taurus (cattle) / Tissue: BLOOD / References: UniProt: P02070 #3: Chemical | ChemComp-HEM / #4: Chemical | ChemComp-CMO / #5: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.35 Å3/Da / Density % sol: 47.56 % | |||||||||||||||||||||||||
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Crystal grow | Temperature: 296 K / Method: batch / pH: 5 Details: sodium cacodylate, sodium dithionite, polyethylene glycol 3350, pH 5.0, Batch, temperature 296K | |||||||||||||||||||||||||
Crystal grow | *PLUS Method: batch method | |||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 298 K |
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Diffraction source | Source: ROTATING ANODE / Type: RIGAKU RU200 / Wavelength: 1.5418 |
Detector | Type: SDMS / Detector: AREA DETECTOR / Date: Sep 1, 1991 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.5418 Å / Relative weight: 1 |
Reflection | Resolution: 1.89→31.69 Å / Num. all: 47248 / Num. obs: 45723 / % possible obs: 96.8 % / Observed criterion σ(F): 0 / Observed criterion σ(I): 0 / Redundancy: 7.3 % / Biso Wilson estimate: 29.5 Å2 / Rmerge(I) obs: 0.058 / Net I/σ(I): 29.45 |
Reflection shell | Resolution: 1.89→2.03 Å / Redundancy: 3.4 % / Rmerge(I) obs: 0.232 / Num. unique all: 9280 / % possible all: 85.3 |
Reflection | *PLUS Num. measured all: 334873 |
Reflection shell | *PLUS % possible obs: 85.3 % |
-Processing
Software |
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Refinement | Resolution: 1.9→8 Å / σ(F): 2 / σ(I): 0 / Stereochemistry target values: Engh & Huber
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Refinement step | Cycle: LAST / Resolution: 1.9→8 Å
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Refine LS restraints |
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Software | *PLUS Name: PROLSQ / Classification: refinement | ||||||||||||||||
Refinement | *PLUS | ||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||
Displacement parameters | *PLUS Biso mean: 31.2 Å2 |