negative regulation of DNA recombination at telomere / protection from non-homologous end joining at telomere / negative regulation of telomere maintenance / shelterin complex / telomere maintenance via telomere lengthening / Telomere C-strand synthesis initiation / Telomere C-strand (Lagging Strand) Synthesis / nuclear telomere cap complex / G-rich strand telomeric DNA binding / telomere capping ...negative regulation of DNA recombination at telomere / protection from non-homologous end joining at telomere / negative regulation of telomere maintenance / shelterin complex / telomere maintenance via telomere lengthening / Telomere C-strand synthesis initiation / Telomere C-strand (Lagging Strand) Synthesis / nuclear telomere cap complex / G-rich strand telomeric DNA binding / telomere capping / Processive synthesis on the C-strand of the telomere / Polymerase switching on the C-strand of the telomere / Removal of the Flap Intermediate from the C-strand / regulation of telomere maintenance / protein localization to chromosome, telomeric region / regulation of double-strand break repair via homologous recombination / nuclear chromosome / telomeric DNA binding / positive regulation of telomere maintenance / negative regulation of protein phosphorylation / Telomere Extension By Telomerase / telomere maintenance via telomerase / phosphatase binding / Packaging Of Telomere Ends / Recognition and association of DNA glycosylase with site containing an affected purine / Cleavage of the damaged purine / Recognition and association of DNA glycosylase with site containing an affected pyrimidine / Cleavage of the damaged pyrimidine / Inhibition of DNA recombination at telomere / Meiotic synapsis / telomere maintenance / male germ cell nucleus / positive regulation of non-canonical NF-kappaB signal transduction / DNA Damage/Telomere Stress Induced Senescence / positive regulation of NF-kappaB transcription factor activity / positive regulation of canonical NF-kappaB signal transduction / chromosome, telomeric region / intracellular signal transduction / nuclear body / regulation of DNA-templated transcription / nucleoplasm / nucleus / cytoplasm 類似検索 - 分子機能
The submitted conformer models are 25 structure with the lowest energy in those with the fewest number of constraint violations.
代表モデル
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要素
#1: タンパク質
TRF2-INTERACTINGTELOMERICRAP1PROTEIN
分子量: 6660.511 Da / 分子数: 1 / 断片: MYB-DOMAIN / 変異: 25MDR / 由来タイプ: 合成 詳細: THIS PEPTIDE WAS CHEMICALLY SYNTHESIZED. THE SEQUENCE OF THIS PEPTIDE OCCURS NATURALLY IN HUMANS (HOMO SAPIENS). 参照: UniProt: Q9NYB0
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実験情報
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実験
実験
手法: 溶液NMR
NMR実験
Conditions-ID
Experiment-ID
Solution-ID
タイプ
1
1
1
2D NOESY
1
2
1
DQF-COSY
NMR実験の詳細
Text: This structure was determined using standard 2D homonuclear techniques.
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試料調製
詳細
内容: 1.0mM protein concentration in 100mM potassium phosphate buffer pH5.5, 1mM NaN3 ; 90%H2O, 10%D2O 溶媒系: 90% H2O/10% D2O
試料状態
pH: 5.5 / 圧: 1 atm / 温度: 300 K
結晶化
*PLUS
手法: other / 詳細: NMR
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NMR測定
放射
プロトコル: SINGLE WAVELENGTH / 単色(M)・ラウエ(L): M
放射波長
相対比: 1
NMRスペクトロメーター
タイプ: Bruker DMX / 製造業者: Bruker / モデル: DMX / 磁場強度: 600 MHz
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解析
NMR software
名称
バージョン
開発者
分類
NMRPipe
4.2.5
Delaglio, F., Grzesiek, S., Vuister, G.W., Zhu,G., Pfeifer, J. andBax,A.
解析
X-PLOR
3.851
Brnger,A.T.
構造決定
X-PLOR
3.851
Brnger,A.T.
精密化
精密化
手法: distance geometry simulated annealing / ソフトェア番号: 1 詳細: the structures are based on a total of 884 restraints, 838 are NOE-derived distance constraints, 39 dihedral angle restraints, 7 distance restraints from hydrogen bonds.
NMRアンサンブル
コンフォーマー選択の基準: The submitted conformer models are 25 structure with the lowest energy in those with the fewest number of constraint violations. 計算したコンフォーマーの数: 100 / 登録したコンフォーマーの数: 25