+データを開く
-基本情報
登録情報 | データベース: PDB / ID: 1f3v | ||||||
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タイトル | Crystal structure of the complex between the N-terminal domain of TRADD and the TRAF domain of TRAF2 | ||||||
要素 |
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キーワード | APOPTOSIS / a-b sandwich | ||||||
機能・相同性 | 機能・相同性情報 CD40 receptor binding / tumor necrosis factor receptor superfamily complex / death domain binding / sphingolipid binding / IRE1-TRAF2-ASK1 complex / Defective RIPK1-mediated regulated necrosis / TRAF2-GSTP1 complex / TRAIL-activated apoptotic signaling pathway / tumor necrosis factor binding / positive regulation of hair follicle development ...CD40 receptor binding / tumor necrosis factor receptor superfamily complex / death domain binding / sphingolipid binding / IRE1-TRAF2-ASK1 complex / Defective RIPK1-mediated regulated necrosis / TRAF2-GSTP1 complex / TRAIL-activated apoptotic signaling pathway / tumor necrosis factor binding / positive regulation of hair follicle development / CD40 signaling pathway / Regulation by c-FLIP / CASP8 activity is inhibited / Dimerization of procaspase-8 / interleukin-17-mediated signaling pathway / TNF receptor superfamily (TNFSF) members mediating non-canonical NF-kB pathway / negative regulation of glial cell apoptotic process / TNF signaling / Caspase activation via Death Receptors in the presence of ligand / programmed necrotic cell death / CD40 receptor complex / regulation of immunoglobulin production / signal transduction involved in regulation of gene expression / positive regulation of tumor necrosis factor-mediated signaling pathway / death-inducing signaling complex / mitogen-activated protein kinase kinase kinase binding / transmembrane receptor protein tyrosine kinase adaptor activity / tumor necrosis factor receptor binding / thioesterase binding / mRNA stabilization / positive regulation of extrinsic apoptotic signaling pathway / vesicle membrane / TNFR1-induced proapoptotic signaling / non-canonical NF-kappaB signal transduction / RIPK1-mediated regulated necrosis / TRAF6 mediated IRF7 activation / TRAF6 mediated NF-kB activation / protein K63-linked ubiquitination / regulation of JNK cascade / intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress / extrinsic apoptotic signaling pathway via death domain receptors / cellular response to nitric oxide / regulation of protein-containing complex assembly / protein autoubiquitination / canonical NF-kappaB signal transduction / signaling adaptor activity / ubiquitin ligase complex / positive regulation of JUN kinase activity / extrinsic apoptotic signaling pathway / tumor necrosis factor-mediated signaling pathway / response to endoplasmic reticulum stress / positive regulation of interleukin-2 production / TNFR1-induced NF-kappa-B signaling pathway / Regulation of NF-kappa B signaling / TNFR2 non-canonical NF-kB pathway / positive regulation of DNA-binding transcription factor activity / Regulation of TNFR1 signaling / RING-type E3 ubiquitin transferase / protein catabolic process / kinase binding / Regulation of necroptotic cell death / positive regulation of T cell cytokine production / cytoplasmic side of plasma membrane / positive regulation of inflammatory response / ubiquitin-protein transferase activity / ubiquitin protein ligase activity / cellular response to tumor necrosis factor / positive regulation of NF-kappaB transcription factor activity / cell cortex / protein-macromolecule adaptor activity / protein-containing complex assembly / protein phosphatase binding / regulation of apoptotic process / positive regulation of canonical NF-kappaB signal transduction / cytoskeleton / receptor complex / Ub-specific processing proteases / positive regulation of cell migration / positive regulation of apoptotic process / membrane raft / innate immune response / ubiquitin protein ligase binding / protein-containing complex binding / apoptotic process / protein kinase binding / enzyme binding / signal transduction / zinc ion binding / nucleoplasm / identical protein binding / nucleus / plasma membrane / cytosol / cytoplasm 類似検索 - 分子機能 | ||||||
生物種 | Homo sapiens (ヒト) | ||||||
手法 | X線回折 / シンクロトロン / 解像度: 2 Å | ||||||
データ登録者 | Park, Y.C. / Ye, H. / Hsia, C. / Segal, D. / Rich, R. / Liou, H.-C. / Myszka, D. / Wu, H. | ||||||
引用 | ジャーナル: Cell(Cambridge,Mass.) / 年: 2000 タイトル: A novel mechanism of TRAF signaling revealed by structural and functional analyses of the TRADD-TRAF2 interaction. 著者: Park, Y.C. / Ye, H. / Hsia, C. / Segal, D. / Rich, R.L. / Liou, H.C. / Myszka, D.G. / Wu, H. | ||||||
履歴 |
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-構造の表示
構造ビューア | 分子: MolmilJmol/JSmol |
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-ダウンロードとリンク
-ダウンロード
PDBx/mmCIF形式 | 1f3v.cif.gz | 78.1 KB | 表示 | PDBx/mmCIF形式 |
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PDB形式 | pdb1f3v.ent.gz | 62.2 KB | 表示 | PDB形式 |
PDBx/mmJSON形式 | 1f3v.json.gz | ツリー表示 | PDBx/mmJSON形式 | |
その他 | その他のダウンロード |
-検証レポート
文書・要旨 | 1f3v_validation.pdf.gz | 375.4 KB | 表示 | wwPDB検証レポート |
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文書・詳細版 | 1f3v_full_validation.pdf.gz | 383.5 KB | 表示 | |
XML形式データ | 1f3v_validation.xml.gz | 9.1 KB | 表示 | |
CIF形式データ | 1f3v_validation.cif.gz | 14.3 KB | 表示 | |
アーカイブディレクトリ | https://data.pdbj.org/pub/pdb/validation_reports/f3/1f3v ftp://data.pdbj.org/pub/pdb/validation_reports/f3/1f3v | HTTPS FTP |
-関連構造データ
-リンク
-集合体
登録構造単位 |
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1 |
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単位格子 |
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-要素
#1: タンパク質 | 分子量: 19412.998 Da / 分子数: 1 / 断片: N-TERMINAL DOMAIN / 由来タイプ: 組換発現 / 由来: (組換発現) Homo sapiens (ヒト) / プラスミド: PET / 発現宿主: Escherichia coli (大腸菌) / 参照: UniProt: Q15628 |
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#2: タンパク質 | 分子量: 19718.646 Da / 分子数: 1 / 断片: TRAF DOMAIN / 由来タイプ: 組換発現 / 由来: (組換発現) Homo sapiens (ヒト) / プラスミド: PET / 発現宿主: Escherichia coli (大腸菌) / 参照: UniProt: Q12933 |
#3: 水 | ChemComp-HOH / |
-実験情報
-実験
実験 | 手法: X線回折 / 使用した結晶の数: 1 |
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-試料調製
結晶 | マシュー密度: 2.71 Å3/Da / 溶媒含有率: 54.56 % | |||||||||||||||
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結晶化 | 温度: 293 K / 手法: 蒸気拡散法 / 詳細: VAPOR DIFFUSION, temperature 293K | |||||||||||||||
結晶化 | *PLUS pH: 5.6 | |||||||||||||||
溶液の組成 | *PLUS
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-データ収集
回折 | 平均測定温度: 100 K |
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放射光源 | 由来: シンクロトロン / サイト: NSLS / ビームライン: X4A / 波長: 0.9 |
検出器 | タイプ: ADSC / 検出器: CCD / 日付: 1999年6月1日 |
放射 | プロトコル: SINGLE WAVELENGTH / 単色(M)・ラウエ(L): M / 散乱光タイプ: x-ray |
放射波長 | 波長: 0.9 Å / 相対比: 1 |
反射 | 解像度: 2→20 Å |
反射 | *PLUS % possible obs: 96.9 % / Rmerge(I) obs: 0.036 |
-解析
ソフトウェア |
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精密化 | 解像度: 2→20 Å / σ(F): 2 /
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精密化ステップ | サイクル: LAST / 解像度: 2→20 Å
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ソフトウェア | *PLUS 名称: CNS / 分類: refinement | ||||||||||||
精密化 | *PLUS 最高解像度: 2 Å / 最低解像度: 20 Å / σ(F): 2 / Rfactor obs: 0.229 | ||||||||||||
溶媒の処理 | *PLUS | ||||||||||||
原子変位パラメータ | *PLUS | ||||||||||||
拘束条件 | *PLUS
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