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Yorodumi- PDB-1f0h: Cecropin A(1-8)-magainin 2(1-12) A2 in dodecylphosphocholine micelles -
+Open data
-Basic information
Entry | Database: PDB / ID: 1f0h | ||||||
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Title | Cecropin A(1-8)-magainin 2(1-12) A2 in dodecylphosphocholine micelles | ||||||
Components | CECROPIN A-MAGAININ 2 HYBRID PEPTIDE | ||||||
Keywords | ANTIMICROBIAL PROTEIN / Helix | ||||||
Function / homology | Function and homology information regulation of defense response to virus / defense response to fungus / antibacterial humoral response / defense response to Gram-negative bacterium / killing of cells of another organism / defense response to Gram-positive bacterium / defense response to bacterium / innate immune response / extracellular space / extracellular region Similarity search - Function | ||||||
Method | SOLUTION NMR / Distance Geometry-Dynamical Simulated Annealing Hybrid Method | ||||||
Authors | Oh, D. / Shin, S.Y. / Lee, S. / Kim, Y. | ||||||
Citation | Journal: Biochemistry / Year: 2000 Title: Role of the hinge region and the tryptophan residue in the synthetic antimicrobial peptides, cecropin A(1-8)-magainin 2(1-12) and its analogues, on their antibiotic activities and structures. Authors: Oh, D. / Shin, S.Y. / Lee, S. / Kang, J.H. / Kim, S.D. / Ryu, P.D. / Hahm, K.S. / Kim, Y. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1f0h.cif.gz | 129.7 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1f0h.ent.gz | 103.5 KB | Display | PDB format |
PDBx/mmJSON format | 1f0h.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 1f0h_validation.pdf.gz | 341.6 KB | Display | wwPDB validaton report |
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Full document | 1f0h_full_validation.pdf.gz | 442.7 KB | Display | |
Data in XML | 1f0h_validation.xml.gz | 8.4 KB | Display | |
Data in CIF | 1f0h_validation.cif.gz | 12.9 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/f0/1f0h ftp://data.pdbj.org/pub/pdb/validation_reports/f0/1f0h | HTTPS FTP |
-Related structure data
-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein/peptide | Mass: 2295.918 Da / Num. of mol.: 1 / Mutation: A28L / Source method: obtained synthetically Details: THIS PEPTIDE WAS CHEMICALLY SYNTHESIZED. THE SEQUENCE FOR THIS PEPTIDE IS A HYBRID OF SEQUENCES WHICH OCCUR NATURALLY IN HYALOPHORA CECROPIA (CECROPIA MOTH) AND XENOPUS LAEVIS (AFRICAN CLAWED FROG) References: UniProt: P01507, UniProt: P11006 |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR |
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NMR experiment | Type: 2D NOESY |
-Sample preparation
Details | Contents: DODECYLPHOSPHOCHOLINE-d38 MICELLES / Solvent system: 90% H2O/10% D2O |
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Sample conditions | pH: 4.05 / Temperature: 298 K |
Crystal grow | *PLUS Method: other / Details: NMR |
-NMR measurement
NMR spectrometer | Type: Bruker AVANCE / Manufacturer: Bruker / Model: AVANCE / Field strength: 400 MHz |
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-Processing
NMR software | Name: X-PLOR / Version: 3.851 / Developer: Brunger, A.T. / Classification: refinement |
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Refinement | Method: Distance Geometry-Dynamical Simulated Annealing Hybrid Method Software ordinal: 1 |
NMR ensemble | Conformer selection criteria: structures with the lowest energy Conformers calculated total number: 50 / Conformers submitted total number: 20 |