[English] 日本語
![](img/lk-miru.gif)
- PDB-1euc: CRYSTAL STRUCTURE OF DEPHOSPHORYLATED PIG HEART, GTP-SPECIFIC SUC... -
+
Open data
-
Basic information
Entry | Database: PDB / ID: 1euc | ||||||
---|---|---|---|---|---|---|---|
Title | CRYSTAL STRUCTURE OF DEPHOSPHORYLATED PIG HEART, GTP-SPECIFIC SUCCINYL-COA SYNTHETASE | ||||||
![]() | (SUCCINYL-COA SYNTHETASE, ...) x 2 | ||||||
![]() | LIGASE / GTP-specific | ||||||
Function / homology | ![]() succinate-CoA ligase (GDP-forming) / succinate-CoA ligase (GDP-forming) activity / succinate-CoA ligase complex (ADP-forming) / succinate-CoA ligase complex / Citric acid cycle (TCA cycle) / succinate-CoA ligase (ADP-forming) / succinate-CoA ligase (ADP-forming) activity / succinyl-CoA metabolic process / tricarboxylic acid cycle / nucleotide binding ...succinate-CoA ligase (GDP-forming) / succinate-CoA ligase (GDP-forming) activity / succinate-CoA ligase complex (ADP-forming) / succinate-CoA ligase complex / Citric acid cycle (TCA cycle) / succinate-CoA ligase (ADP-forming) / succinate-CoA ligase (ADP-forming) activity / succinyl-CoA metabolic process / tricarboxylic acid cycle / nucleotide binding / GTP binding / mitochondrion / ATP binding / metal ion binding / cytosol Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() | ||||||
![]() | Fraser, M.E. / James, M.N.G. / Bridger, W.A. / Wolodko, W.T. | ||||||
![]() | ![]() Title: Phosphorylated and dephosphorylated structures of pig heart, GTP-specific succinyl-CoA synthetase. Authors: Fraser, M.E. / James, M.N. / Bridger, W.A. / Wolodko, W.T. | ||||||
History |
|
-
Structure visualization
Structure viewer | Molecule: ![]() ![]() |
---|
-
Downloads & links
-
Download
PDBx/mmCIF format | ![]() | 144.2 KB | Display | ![]() |
---|---|---|---|---|
PDB format | ![]() | 115.7 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 448.1 KB | Display | ![]() |
---|---|---|---|---|
Full document | ![]() | 459.5 KB | Display | |
Data in XML | ![]() | 28.2 KB | Display | |
Data in CIF | ![]() | 40.4 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
-
Links
-
Assembly
Deposited unit | ![]()
| ||||||||
---|---|---|---|---|---|---|---|---|---|
1 |
| ||||||||
Unit cell |
| ||||||||
Details | The biological assembly is an alpha, beta-dimer constructed of chains A and B. |
-
Components
-SUCCINYL-COA SYNTHETASE, ... , 2 types, 2 molecules AB
#1: Protein | Mass: 32803.773 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() References: UniProt: O19069, succinate-CoA ligase (GDP-forming) |
---|---|
#2: Protein | Mass: 42810.996 Da / Num. of mol.: 1 / Mutation: L23V Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() References: UniProt: P53590, succinate-CoA ligase (GDP-forming) |
-Non-polymers , 4 types, 208 molecules ![](data/chem/img/PO4.gif)
![](data/chem/img/SO4.gif)
![](data/chem/img/ZN.gif)
![](data/chem/img/HOH.gif)
![](data/chem/img/SO4.gif)
![](data/chem/img/ZN.gif)
![](data/chem/img/HOH.gif)
#3: Chemical | ChemComp-PO4 / | ||||
---|---|---|---|---|---|
#4: Chemical | #5: Chemical | ChemComp-ZN / | #6: Water | ChemComp-HOH / | |
-Experimental details
-Experiment
Experiment | Method: ![]() |
---|
-
Sample preparation
Crystal | Density Matthews: 2.27 Å3/Da / Density % sol: 45.84 % | |||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Crystal grow | Temperature: 298 K / Method: microdialysis Details: sodium,potassium(1:3) phosphate, 2-mercaptoethanol, ammonium sulfate, MICRODIALYSIS, temperature 298K | |||||||||||||||||||||||||
Crystal grow | *PLUS Temperature: 4 ℃ / PH range low: 7.4 / PH range high: 7.3 | |||||||||||||||||||||||||
Components of the solutions | *PLUS
|
-Data collection
Diffraction | Ambient temp details: 279-283 |
---|---|
Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: WEISSENBERG / Detector: DIFFRACTOMETER / Date: Dec 1, 1993 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1 Å / Relative weight: 1 |
Reflection | Resolution: 2.1→59 Å / Num. all: 37771 / Num. obs: 37771 / % possible obs: 95.4 % / Observed criterion σ(F): 0 / Observed criterion σ(I): 0 / Redundancy: 4 % / Biso Wilson estimate: 21.7 Å2 / Rmerge(I) obs: 0.109 |
Reflection shell | Resolution: 2.09→2.13 Å / Redundancy: 2.5 % / Rmerge(I) obs: 0.307 / Num. unique all: 2027 / % possible all: 71 |
Reflection | *PLUS Num. all: 52273 / Num. measured all: 213996 |
Reflection shell | *PLUS % possible obs: 71 % |
-
Processing
Software |
| |||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Refinement | Resolution: 2.1→59 Å / σ(F): 0 / σ(I): 0 / Stereochemistry target values: Engh & Huber
| |||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.1→59 Å
| |||||||||||||||||||||||||
Refine LS restraints |
|