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Yorodumi- PDB-2scu: A detailed description of the structure of Succinyl-COA synthetas... -
+Open data
-Basic information
Entry | Database: PDB / ID: 2scu | ||||||
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Title | A detailed description of the structure of Succinyl-COA synthetase from Escherichia coli | ||||||
Components | (PROTEIN (SUCCINYL-COA LIGASE)) x 2 | ||||||
Keywords | LIGASE / CITRIC ACID CYCLE / HETEROTETRAMER | ||||||
Function / homology | Function and homology information succinate-CoA ligase complex / succinate-CoA ligase complex (ADP-forming) / succinate-CoA ligase (GDP-forming) activity / succinyl-CoA metabolic process / succinate-CoA ligase (ADP-forming) / succinate-CoA ligase (ADP-forming) activity / tricarboxylic acid cycle / nucleotide binding / magnesium ion binding / ATP binding ...succinate-CoA ligase complex / succinate-CoA ligase complex (ADP-forming) / succinate-CoA ligase (GDP-forming) activity / succinyl-CoA metabolic process / succinate-CoA ligase (ADP-forming) / succinate-CoA ligase (ADP-forming) activity / tricarboxylic acid cycle / nucleotide binding / magnesium ion binding / ATP binding / cytosol / cytoplasm Similarity search - Function | ||||||
Biological species | Escherichia coli (E. coli) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MIR / Resolution: 2.3 Å | ||||||
Authors | Fraser, M.E. / Wolodko, W.T. / James, M.N.G. / Bridger, W.A. | ||||||
Citation | Journal: J.Mol.Biol. / Year: 1999 Title: A detailed structural description of Escherichia coli succinyl-CoA synthetase. Authors: Fraser, M.E. / James, M.N. / Bridger, W.A. / Wolodko, W.T. #1: Journal: J.Biol.Chem. / Year: 1994 Title: The Crystal Structure of Succinyl-Coa Synthetase from Escherichia Coli at 2.5 Angstroms Resolution Authors: Wolodko, W.T. / Fraser, M.E. / James, M.N.G. / Bridger, W.A. #2: Journal: J.Biol.Chem. / Year: 1984 Title: Crystallization of Succinyl-Coa Synthetase from Escherichia Coli Authors: Wolodko, W.T. / James, M.N.G. / Bridger, W.A. #3: Journal: J.Mol.Biol. / Year: 1999 Title: A Dimeric Form of Escherichia Coli Succinyl-Coa Synthetase Produced by Site- Directed Mutagenesis Authors: Bailey, D.L. / Fraser, M.E. / Bridger, W.A. / James, M.N.G. / Wolodko, W.T. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 2scu.cif.gz | 269.2 KB | Display | PDBx/mmCIF format |
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PDB format | pdb2scu.ent.gz | 216.3 KB | Display | PDB format |
PDBx/mmJSON format | 2scu.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/sc/2scu ftp://data.pdbj.org/pub/pdb/validation_reports/sc/2scu | HTTPS FTP |
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-Related structure data
Related structure data | |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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2 |
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Unit cell |
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Components on special symmetry positions |
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Noncrystallographic symmetry (NCS) | NCS oper:
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-Components
#1: Protein | Mass: 29758.213 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Details: RESIDUES A246 AND D246 ARE PHOSPHOHISTIDINES / Source: (gene. exp.) Escherichia coli (E. coli) / Strain: CR63 / Production host: Escherichia coli (E. coli) References: UniProt: P07459, UniProt: P0AGE9*PLUS, succinate-CoA ligase (ADP-forming) #2: Protein | Mass: 41438.496 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Details: RESIDUES A246 AND D246 ARE PHOSPHOHISTIDINES / Source: (gene. exp.) Escherichia coli (E. coli) / Strain: CR63 / Production host: Escherichia coli (E. coli) References: UniProt: P07460, UniProt: P0A836*PLUS, succinate-CoA ligase (ADP-forming) #3: Chemical | #4: Chemical | #5: Water | ChemComp-HOH / | Nonpolymer details | SO4 400 IS ASSOCIATED | Sequence details | AMINO-TERMINAL ANALYSIS OF THE ALPHA SUBUNIT INDICATES THAT THE FIRST RESIDUE IS A SERINE (W. A. ...AMINO-TERMINAL ANALYSIS OF THE ALPHA SUBUNIT INDICATES THAT THE FIRST RESIDUE IS A SERINE (W. A. BRIDGE, ENZYMES, 1974, 3RD ED. 10, 581-606). IN THE GENE SEQUENCING | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 23 |
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-Sample preparation
Crystal | Density Matthews: 3.45 Å3/Da / Density % sol: 64.35 % Description: DATA WERE COLLECTED USING THE WEISSENBERG METHOD. | |||||||||||||||||||||||||||||||||||
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Crystal grow | pH: 7.2 / Details: pH 7.2 | |||||||||||||||||||||||||||||||||||
Crystal grow | *PLUS Temperature: 21 ℃ / pH: 7.3 / Method: microdialysis / Details: Wolodko, W.T., (1984) J. Biol. Chem., 259, 5316. | |||||||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 283 K |
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Diffraction source | Source: SYNCHROTRON / Site: Photon Factory / Beamline: BL-6A / Wavelength: 1 |
Detector | Detector: IMAGE PLATE / Date: Mar 1, 1995 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1 Å / Relative weight: 1 |
Reflection | Resolution: 2.3→100 Å / Num. obs: 417950 / % possible obs: 92.7 % / Observed criterion σ(I): 0 / Redundancy: 5 % / Biso Wilson estimate: 32 Å2 / Rmerge(I) obs: 0.095 |
Reflection shell | Resolution: 2.3→2.5 Å / Rmerge(I) obs: 0.45 |
Reflection | *PLUS Num. obs: 83411 / Num. measured all: 417950 |
-Processing
Software |
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Refinement | Method to determine structure: MIR / Resolution: 2.3→8 Å / σ(F): 0
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Refinement step | Cycle: LAST / Resolution: 2.3→8 Å
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Refine LS restraints |
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Software | *PLUS Name: TNT / Version: 5EB / Classification: refinement | ||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement | *PLUS Rfactor all: 0.195 | ||||||||||||||||||||||||||||||||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints | *PLUS
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